Preparation and adsorption behaviors of Cu(II) ion-imprinted polymers

Imprinted polymers were prepared for selective removal of Cu（Ⅱ） ions from metal solutions. Three ion-imprinted polymers were synthesized with methacrylic acid （MAA）, acrylamide （AA） and N,N＇-methylenebisacrylamide （MBAA） respectively as the functional monomers, ethleneglycoldimethacrylate （EGDMA） as the cross-linking agent, 2,2＇- azobisisobutyronitrile （AIBN） as the initiator and Cu （Ⅱ） ion as the imprint ion. The template Cu （Ⅱ） ion was removed from the polymer by leaching with a liquid of a 1：1 volumetric ratio of HCl to ethylenediaminetetraacetic acid （EDTA）. The capacity and selectivity of Cu（Ⅱ） ion adsorption were investigated with the three imprinted polymers and their non-imprinted counterparts. The polymers have a maximum adsorption capacity at pH 7.0. The isotherm of their batch adsorption of Cu（Ⅱ） ions shows a Langmuir adsorption pattern. Imprinted polymers all have a much higher capacity and higher selectivity of Cu（Ⅱ） adsorption than nonimprinted ones. MAA polymer benefits the most from imprinting. Imprinted MAA polymer has the highest selectivity when used to rebind Cu （Ⅱ） ion from an aqueous solution in the presence of other metal ions. Ion imprinting can be a promising technique of preparing selective adsorbents to separate and preconcentrate metal in a medium of multiple competitive metal ions through solid phase extraction （SPE）.     

海带多酚提取工艺优化及抗氧化性研究

以海带多酚为研究对象,考察提取温度、料液比和超声时间对海带游离多酚和结合多酚提取率的影响、采用正交试验对多酚提取工艺进行优化,并通过测定多酚对DPPH自由基的清除能力评估其抗氧化性.结果表明,海带多酚的最佳提取工艺为:料液比为1:30(g/mL)、提取温度为40℃、超声时间为30 min.在此工艺条件下,海带总多酚的提取率为2.74%.当浓度为2.0 mg/mL时,游离多酚和结合多酚对DPPH·的清除率分别为41.72%和19.37%,表明海带多酚具有一定的抗氧化活性,且游离多酚的抗氧化活性高于结合多酚。     

Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.     

钉螺血蓝蛋白的提取与性质研究

利用超高速冷冻离心和Sepharose 4B凝胶过滤两步法从日本血吸虫中间寄主-湖北钉螺血淋巴中提取获得一种含铜呼吸蛋白-钉螺血蓝蛋白.该血蓝蛋白具有典型的血蓝蛋白基本特征,含铜量为0.23%,在340nm处观测到该蛋白的紫外特征吸收峰.SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测该呼吸蛋白由两种蛋白质亚基组成,分子量分别为400KDa和250KDa.透射电子显微镜测定结果证实其四级结构为中凹的圆柱体,且以单体、二聚体和多聚体形式共存于血淋巴中.     

基于投影图与直方图的机械零件模型检索

为实现资源重复利用与产品创新,通过检索出数据库中的相似零件给设计者提供帮助。首先对机械零件模型进行方位归一化与预处理,以起始点为圆心作最大内切圆,划分连通区,在连通区内根据距离变换值判定邻域像素,进而确定新的骨架点,迭代生成完整骨架。将骨架转换成直方图曲线,划分网格生成骨架点数矩阵,根据矩阵特征值和之间的差计算两模型间的差异度,从而判定机械零件相似度。通过实例验证以及与 D2形状分布算法及递归分割算法的比较,该方法检索速度高于递归分割算法,准确性高于 D2 形状分布算法和递归分割算法。     

炼油行业油泥分离工艺的实验研究

针对中石化九江分公司含油污泥的性状及成分结构,对含油污泥分别进行了溶剂热萃取、微波处理方法的实验研究,并对其最佳工艺条件进行了探讨,制定相应的工艺路线,比较两种工艺的优劣,得出微波处理油泥工艺具有发展优势的结论。     

基于CISnet的信息抽取分析

信息抽取的目标是自动从文本信息中抽取出预先想要得到的信息（知识），它提供了一条从浩瀚的信息堆积中抽取出与用户相关信息的一条思路。文章分析了信息抽取的主要概念、主要分析了信息抽取的类型和信息抽取系统的一般结构及功能。     

我国页岩气开发技术工程化实现的学习曲线研究

技术学习是实现页岩气开发技术工程化的重要途径,开发技术工程化是实现规模化商业开发的前提。页岩气开发极大地区别于常规油气,具有显著的技术学习效应。依据涪陵页岩气田实地调研数据,拟合不同开发阶段对应井别的钻井总成本学习曲线,并与传统的技术学习曲线进行了对比分析。研究发现,开发早期相对中后期,技术学习速率更高,钻井成本变动更剧烈;考虑规模效应时,钻井成本变动较为平缓,但技术学习的间接作用则更为明显。     

基于触发词优先级的事件抽取研究

本文将触发词分为时间类和非时间类,对触发词提取算法进行改进,以一定量导电塑料行业新闻为基础语料构建两类触发词词表,并采取时间类触发词优先的事件句识别策略。基于该触发词词表对导电塑料和太阳能行业新闻语料进行事件句识别算法有效性实验,开放测试的召回率和准确率分别超过98%和95%。该结果表明：将触发词进行基于时间特性的分类,并优先使用时间类触发词提取事件句,能取得显著的效果。     

ARMA模型在远程心电诊断中的应用研究

研究适于远程心电诊断，基于ARMA模型的高精度的心电信号(ECG)直接分类方法．利用ARMA模型系数作为特征对ECG信号进行分类和压缩．在对信号特征分类时，采用了BP神经网络分类的方法．利用文中方法对MIT-BIH标准数据库中的正常窦性心律(NSR)、心房早期收缩(APC)、心室早期收缩(PVC)、心室性心动过速(VT)、室上性心动过速(SVT)和心室纤维性颤动(VF)各300个样本信号进行了测试，获得了96．51％～98．38％的分类精度．