A study of serum zinc, selenium and copper levels in carcinoma of esophagus patients

The association of serum trace elements like selenium, zinc and copper has been found in different types of cancer. This study was conducted to see the serum level of these three trace elements in cancer esophagus patients. Biopsy confirmed cancer esophagus, 24 patients (12 males, 12 females, mean age 54.5±11.65 year with 23 healthy subjects (16 males, 7 females, mean age 44 ±13.82 years) were included in this study. Both control and study group patients were of same socio-economic status and dietary habits. Serum zinc and copper level were estimated using standard absorption spectrometer technique and serum selenium by Hydride generation method. We observed significant low serum levels of zinc and selenium while high level of serum copper in carcinoma esophagus patients, as compared with normal healthy controls. This shows an association of serum selenium zinc and copper with cancer esophagus.     

半胱胺对肉鸡部分血清化学参数及酶活性的影响

144只一日龄的AA肉仔鸡随机分为4组(n=36),预饲一周后分别在试验组基础日粮中添加50mg/kg、100mg/kg、150mg/kg的半胱胺,分别于2、4、6周龄采颈静脉血,分离血清,测定四组血清钙、磷和葡萄糖含量及ALT、AST、ALP活性,研究半胱胺对肉鸡部分血清化学参数及酶活性的影响.结果表明,饲料中添加适量的Cs能升高AA肉鸡的血钙水平,而对鸡血清磷、葡萄糖含量没有明显影响;添加50～100mg/kg的Cs对ALT、AST活性没有明显的影响;而饲料中添加Cs对ALP活性有一定影响.     

高强度运动对血清IL-8表达水平的影响

目的：探讨血清IL-8表达水平与运动的相关性。方法：用酶联免疫吸附试验（ELISA双抗体夹心法）检测12名研究对象运动前后IL-8的血清水平。结果：12名研究对象运动后的IL-8血清水平较运动前有明显提高（P〈0．05）。结论：本研究结果显示运动对IL-8的表达有明显影响。IL-8在炎症及疾病发展过程中起到了重要的作用,本研究显示高强度运动对免疫系统的影响有临床应用价值。     

皖南花猪血清酶的测定和分析

本研究测定了皖南花猪从出生到３６０日龄血清７种酶的水平和活性,并分析了随猪日龄变化各种酶活性的变化规律。结果显示,各种酶随着日龄的变化十分明显,其中３０～６０和１２０～１５０日龄阶段是各种酶变化的转折点：猪去势后这些酶的变化趋势无明显的改变。在后备猪中仅ＡＫＰ、ＧＯＴ和ＣＫ分别与日龄之间的相关达到显著水平（－０．７５３２、－０．７１２１和－０．７５７５）,酶之间的相关仅ＧＯＴ与ＡＫＰ之间表现明显（０．８９９０）;而肥育猪中Ａｍｙ、ＬＤＨ和ＣＫ分别与日龄的相关达到－０．７２９８、－０．９３５７和－０．７６２６,ＧＯＴ分别与ＡＫＰ和Ａｍｙ的相关达到０．７３４９和０．９１１３,ＬＤＨ分别与Ａｍｙ和ＣＫ的相关达到０．７４０３和０．７４８１。     

水牛肝片吸虫感染后机体无机盐变化的研究

实验选择12头、2-3岁、体重300-500kg雄性去势水牛,经粪便检查和DotELISA检查肝片吸虫阴性,随机分成感染组(n=9)和对照组(n=3),感染组每头一次口服1600个囊蚴.每周定时分别从水牛颈静脉采集感染前0周和感染后25周血液一次,分离血清,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量,旨在研究感染肝片吸虫对水牛机体无机盐含量的影响.结果表明感染后水牛血清中的钾、钠、钙、镁、磷、氯的含量均成波状线变化,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化,这与虫体感染后机体的代谢发生改变有关.     

专项训练对安静时血清氨基酸水平影响的研究

通过测定普通学生、举重和长泳运动员安静时血清氨基酸发现，有训练者多数氨基酸代谢有较高水平，尤其是与一碳单位代谢和与脂代谢有关的氨基酸水平出现一致性有意义的升高；力量训练者蛋氨酸和赖氨酸水平高于耐力训练者。提示训练和专项训练对安静时血清氨基酸水平均有一定影响。     

牛血清白蛋白荧光猝灭法测定药物中的头孢他美酯

[目的]应用牛血清白蛋白荧光猝灭法建立一种测定药物中头孢他美酯含量的新方法。[方法]牛血清白蛋白（BSA）具有很强的内源荧光性,而头孢他美酯溶液本身不产生荧光。当头孢他美酯与BSA结合后,会导致其荧光强度下降。BSA在λex=342nm处的荧光猝灭程度与头孢他美酯的量在一定浓度范围内呈良好的线性关系,据此建立测定药品中头孢他美酯含量的新方法。[结果]该结合物的最大发射波长为λmax=342nm,与头孢他美酯摩尔浓度在2．07×10^-6-2．58×10—5mol·L^-1范围内线性关系良好。其线性回归方程为△F=1．9684×10^7Ccp＋25．6288,相关系数r=0．9958,检出限为1．01×10^-7mol·L^-1,RSD为0．66％,加标回收率为85．0％-100．99／6。[结论]本方法操作简便、快速,用于实际样本的测定,结果满意。     

茜素黄R与牛血清白蛋白光谱学及分子对接研究

在中性条件下,利用紫外可见分光光谱法和分子对接技术研究了偶氮类小分子染料茜素黄R与牛血清白蛋白的相互作用。紫外可见光谱表明,茜素黄R与牛血清白蛋白键合形成的复合物最大吸收波长为386nm,比茜素黄R红移了13nm,二者的结合摩尔比约为1:1,表观摩尔吸光系数为8.70×103dm3·mol-1·cm-1。结合分子模拟技术研究复合物的结合模式和键合机制,判定茜素黄R在牛血清白蛋白上的结合位置位于site I结合位点,讨论二者之间的作用力以静电作用为主,同时也包括疏水作用和氢键。分子对接模拟结果与实验结论一致。     

Correlations between serum and salivary hormonal concentrations in response to resistance exercise

Abstract

The aim of present study was to examine the relationships between serum and salivary values of free testosterone, dehydroepiandrosterone, and cortisol before and after a session of resistance exercise. Twenty-eight healthy men (mean age 40 years, s = 4) participated in the present study. Serum and salivary samples were collected at rest and after a multiple-sets resistance exercise protocol, of approximately 25 minutes duration. Concentrations of free testosterone, dehydroepiandrosterone, and cortisol were measured using radioimmunoassay kits. No significant correlation was observed between serum free testosterone and salivary testosterone (r = 0.22 to 0.26, P > 0.05). Serum cortisol was significantly correlated with salivary cortisol before (r = 0.52, P = 0.005) and after (r = 0.62, P = 0.001) the exercise protocol. Serum and salivary concentrations of dehydroepiandrosterone were significantly correlated before (r = 0.68, P < 0.001) and after (r = 0.7, P < 0.001) exercise. The results of the present study suggest that even under exercise conditions, the salivary values of cortisol and dehydroepiandrosterone can reflect the behaviour of these hormones in blood. However, further studies are necessary to verify if salivary testosterone reflects the behaviour of serum free testosterone during resistance exercise.