Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline

At 0.12 mmol/L γ-glutamyl p-nitroaniline (GGPNA), an improved integrated method was developed for kinetic analysis of γ-glutamyltransferase (GGT) reaction process and the integration with the classical initial rate method to measure serum GGT. For the improved integrated method, an integrated rate equation, which used the predictor variable of reaction time and considered inhibitions by both GGPNA and products, was nonlinearly fit to GGT reaction processes. For the integration strategy, classical initial rates were estimated when GGPNA consumption percentages were below 50%; otherwise, maximal reaction rates of GGT were estimated by the improved integrated method and converted into initial rates according to the differential rate equation at 0.11 mmol/L GGPNA. The integration strategy was validated using optimized GGT kinetic parameters and 10-s intervals to record reaction curves within 8.0 min. By the integration strategy, there was a linear response from 0.9 to 32.0 U/L GGT, coefficients of variation were below 3.5% for GGT from 8.0 to 32.0 U/L (n=5), and GGT activities in clinical sera responded linearly to their classical initial rates at 2.00 mmol/L GGPNA with an expected slope. Therefore, the integration strategy was successful in measuring GGT at 0.12 mmol/L GGPNA.     

The Correlative Study of Serum Pseudo-cholinesterase, Biological Parameters and Symptoms Among Occupational Workers

Cholinesterase is one of many important enzymes needed for acetylcholine hydrolysis. This study aimed to evaluate the serum pseudo-cholinesterase (BuChE) level and its relation to biological parameters (hematological and biochemical parameters) and symptoms among occupational workers, gasoline station worker in Bangkok, Thailand. Our results revealed that the average BuChE level was in normal range, but it had strong relation to hemoglobin (Hb), hematocrit (Hct), blood urea nitrogen (BUN), creatinine (Cr) and serum glutamic pyruvic trans-aminase (SGPT) (Linear regression analysis, p < 0.05, p < 0.05, p < 0.01, p = 0.001 and p < 0.01 respectively). Although, BuChE was not related to white blood cell parameters, but it may exhibit the skin irritation (p = 0.058) of the workers as it showed significant correlation to conjunctivitis and nausea (Pearson’s correlation, p < 0.01 and p < 0.05). In conclusion, this finding may explain that BuChE was significantly related to red blood cells (RBC), liver and kidney functions.     

孝感市城区3～4岁儿童血清锌、硒微量元素含量调查研究

目的：描述孝感市城区学龄前儿童微量元素锌、硒含量分布,为制定儿童微量元素干预措施提供依据。方法：幼儿园3～4岁儿童作为研究对象,采用原子吸收法测儿童血清锌、硒两种微量元素含量。用频数分布表和频数分布图描述微量元素含量分布,用SPSS12软件作微量元素含量偏低率和偏高率的统计。结果：293例孝感市城区3～4岁儿童血清锌元素含量在9～12umol／L所占比例最高,为21．50％;血清硒元素含量在2．0～2．5umol／L所占比例最高,为24．57％;血清锌、硒元素含量偏低率分别为11．94％、18．09％。     

耐力训练对安静状态和短时渐增负荷下血清氨基酸影响的研究

通过测定普通学生和长泳运动员安静状态和短时渐增负荷下血清氨基酸后发现：长泳运动员安静时血清氨基酸水平高于普通学生；与有氧供能过程中关键酶活性密切相关的丝氨酸和甘氨酸水平明显高于普通学生；短时渐增负荷下，长泳运动员较之普通学生存在更为活跃的支链氨基酸－丙氨酸－葡萄糖循环活动。结果提示，安静状态和短时渐增负荷下，某些氨基酸代谢活动对长期耐力训练有适应性改变。     

肝片吸虫感染对山羊机体无机盐含量的影响

选择 9只 6 - 10月龄的健康雄性白山羊 ,经粪便检查和DotBLISA检测 ,确认无肝片吸虫感染 ,随机分成感染组 (n =5 )和对照组 (n =4 ) ,试验组每只一次口服接种 15 0个肝片吸虫囊蚴。每周定时从颈静脉采集感染前 0周和感染后 14周血液一次 ,分离血清 ,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量 ,旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化 ,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化 ,这与虫体感染后机体的代谢发生改变有关。     

Acute phase reactants, challenge in the near future of animal production and veterinary medicine

INTRODUCTION The systemic acute phase reaction known to occur on infection, inflammation, trauma, burns, ma- lignancies and tissue damage in general, has been studied by scientists from various disciplines. In the last decade, emphasis has been laid on application of blood tests for acute phase reactants to monitor ani-mal health in general, as well as for human patients suffering from specified classes of diseases (Gruys et al., 2005). However, basic mechanistic patterns as- sociated wit…     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

孝感市城区幼儿园3～4岁儿童血清铁含量调查研究

目的：描述孝感市城区学龄前儿童血清铁元素含量分布,为制定儿童铁微量元素干预措施提供依据。方法：城区幼儿园3—4岁儿童作为研究对象,采用原子吸收法测定儿童血清铁元素含量。所有数据采用SPSS12分析软件统计分析,用频数分布表和频数分布图描述含量分布。结果：孝感市城区3—4岁儿童血清铁元素的平均水平为21．03μmol／L。儿童血清铁元素含量在10～15μmol／L所占比例最高,为22．87％。孝感市城区儿童血清铁含量偏低率为11．26％,偏高率为15.4％。     

血清镍铬元素含量检测用于镍铬合金烤瓷冠修复的研究

目的：研究镍铬合金烤瓷冠修复后血清中镍铬元素含量的变化，探讨镍铬合金长期存在于口腔中的安全性。方法：选取60名经体检合格且无任何病史的健康人作为正常对照组，采用石墨炉原子吸收光谱法测得其血清中镍铬元素含量；再从我院修复科选取60名上、下颌前牙有任意三颗行镍铬合金烤瓷冠修复后半年和一年的血清中镍铬元素含量作为实验组，并对实验组和对照组的镍铬元素含量进行分析。结果：血清镍含量：实验半年组与对照组比较无显著性差异（P〉0．05）；实验一年组与对照组相比较有显著性差异（P〈0．05）；实验一年组与半年组比较无显著性差异（P〉0．05）。血清铬含量：各组之间比较均无显著性差异（P〉0．05）。结论：镍铬合金烤瓷冠修复后会在口腔环境中释放一定的镍和铬，但量很微小，远低于对人体产生毒副作用的水平。     

人血清白蛋白与番红花红T的相互作用研究

研究了人血清白蛋白与番红花红T的结合反应特征。荧光光谱显示番红花红T对人血清白蛋白的荧光有猝灭作用。用Stern-Volmer方程和Lineweaver-Burk双倒数函数处理实验数据。得到一定温度下的动态猝灭常数和静态猝灭结合常数、反应的热力学参数和结合位点数。根据Fǒster能量转移原理计算了番红花红T与人血清白蛋白的结合距离r=2.80nm。