糖尿病患者血清中微量元素的分析研究

对 ６５例糖尿病患者进行了血清中微量元素锌、铜、铁、铬、锰的含量测定,并与正常对照组进行了比较．结果表明,病例组的糖尿病患者血清锌、铁、铬、锰均低于对照组,其中锌、铬、锰水平与对照组比较差异显著（Ｐ＜０．０５）;血清铁虽低于对照组,但无统计学意义．血清钢水平则高于对照组,铜水平与对照组比较差异显著（Ｐ＜０．０５）．     

Serum ionised calcium in birth asphyxia

Serum total and ionised calcium levels were measured at birth and at 48 hours in 25 term neonates with birth asphyxia (one minute APGAR score of 6 or less) and in 25 normal term neonates (one minute APGAR score of 7 or more). Infants were categorised into two groups TAGA (term appropriate for gestational age) and TSGA (term small for gestational age). Asphyxiated infants had significantly lower serum total and ionised calcium values at birth as well as at 48 hours. Abnormal clinical features were observed in 48% of asphyxiated infants. Low ionised calcium was detected in symptomatic babies, who had otherwise normal total calcium values. Due to hyocalcemia especially ionised calcium in asphyxiated infants and high frequency of functional derangement associated with this hypocalcemioa, serial monitoring of serum isonised calcium levels is necessary.     

皖南花猪血清酶的测定和分析

本研究测定了皖南花猪从出生到３６０日龄血清７种酶的水平和活性,并分析了随猪日龄变化各种酶活性的变化规律。结果显示,各种酶随着日龄的变化十分明显,其中３０～６０和１２０～１５０日龄阶段是各种酶变化的转折点：猪去势后这些酶的变化趋势无明显的改变。在后备猪中仅ＡＫＰ、ＧＯＴ和ＣＫ分别与日龄之间的相关达到显著水平（－０．７５３２、－０．７１２１和－０．７５７５）,酶之间的相关仅ＧＯＴ与ＡＫＰ之间表现明显（０．８９９０）;而肥育猪中Ａｍｙ、ＬＤＨ和ＣＫ分别与日龄的相关达到－０．７２９８、－０．９３５７和－０．７６２６,ＧＯＴ分别与ＡＫＰ和Ａｍｙ的相关达到０．７３４９和０．９１１３,ＬＤＨ分别与Ａｍｙ和ＣＫ的相关达到０．７４０３和０．７４８１。     

水牛肝片吸虫感染后机体无机盐变化的研究

实验选择12头、2-3岁、体重300-500kg雄性去势水牛,经粪便检查和DotELISA检查肝片吸虫阴性,随机分成感染组(n=9)和对照组(n=3),感染组每头一次口服1600个囊蚴.每周定时分别从水牛颈静脉采集感染前0周和感染后25周血液一次,分离血清,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量,旨在研究感染肝片吸虫对水牛机体无机盐含量的影响.结果表明感染后水牛血清中的钾、钠、钙、镁、磷、氯的含量均成波状线变化,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化,这与虫体感染后机体的代谢发生改变有关.     

高强度运动对血清IL-8表达水平的影响

目的：探讨血清IL-8表达水平与运动的相关性。方法：用酶联免疫吸附试验（ELISA双抗体夹心法）检测12名研究对象运动前后IL-8的血清水平。结果：12名研究对象运动后的IL-8血清水平较运动前有明显提高（P〈0．05）。结论：本研究结果显示运动对IL-8的表达有明显影响。IL-8在炎症及疾病发展过程中起到了重要的作用,本研究显示高强度运动对免疫系统的影响有临床应用价值。     

大豆皂甙饮料缓解体力疲劳作用的实验研究

目的:研究大豆皂甙饮料的缓解体力疲劳作用。方法:采用雄性ICR小鼠,大豆皂甙饮料按33.3ml/kg.bw、66.7ml/kg.bw、100.0ml/kg.bw连续灌胃30d,测定小鼠负重游泳时间、血乳酸、血清尿素氮和肝糖原含量。结果:100.0ml/kg.bw剂量组小鼠负重游泳时间显著长于对照组(P<0.05).66.Tml/kg.bw剂量组游泳后0min血乳酸含量和3个时点血乳酸曲线下面积显著低于对照组(P<0.05、P<0.01)。33.3ml/kg.bw剂量组肝糖原含量显著高于对照组(P相似文献   

镍铬合金烤瓷冠修复对患者头发和血液中镍铬元素含量的影响

目的:观察镍铬合金烤瓷冠修复后头发和血液中镍铬元素含量的变化,探讨镍铬合金长期存在于口腔中的安全性。方法:选取60名经体检合格的健康人作为正常对照组;60名上、下颌前牙有任意三颗行镍铬合金烤瓷冠修复的患者作为实验组。实验组修复后半年及一年和对照组均采用火焰原子吸收光谱法测定头发镍铬元素含量,采用石墨炉原子吸收光谱法测得其血清中镍铬元素含量。结果:头发镍含量:实验半年组与对照组比较无显著性差异(P>0.05);实验一年组与对照组相比较有显著性差异(P<0.05);实验一年组与半年组比较无显著性差异(P>0.05)。头发铬含量:各组之间比较均无显著性差异(P>0.05)。血清镍含量:实验半年组与对照组比较无显著性差异(P>0.05);实验一年组与对照组相比较有显著性差异(P<0.05);实验一年组与半年组比较无显著性差异(P>0.05)。血清铬含量:各组之间比较均无显著性差异(P>0.05)。结论:镍铬合金烤瓷冠修复后会在口腔环境中释放一定的镍、铬,且释放的量与时间有关,但量很微小,头发和血液中镍铬元素含量呈正相关。     

半胱胺对肉鸡部分血清化学参数及酶活性的影响

144只一日龄的AA肉仔鸡随机分为4组(n=36),预饲一周后分别在试验组基础日粮中添加50mg/kg、100mg/kg、150mg/kg的半胱胺,分别于2、4、6周龄采颈静脉血,分离血清,测定四组血清钙、磷和葡萄糖含量及ALT、AST、ALP活性,研究半胱胺对肉鸡部分血清化学参数及酶活性的影响.结果表明,饲料中添加适量的Cs能升高AA肉鸡的血钙水平,而对鸡血清磷、葡萄糖含量没有明显影响;添加50～100mg/kg的Cs对ALT、AST活性没有明显的影响;而饲料中添加Cs对ALP活性有一定影响.     

A study of serum zinc, selenium and copper levels in carcinoma of esophagus patients

The association of serum trace elements like selenium, zinc and copper has been found in different types of cancer. This study was conducted to see the serum level of these three trace elements in cancer esophagus patients. Biopsy confirmed cancer esophagus, 24 patients (12 males, 12 females, mean age 54.5±11.65 year with 23 healthy subjects (16 males, 7 females, mean age 44 ±13.82 years) were included in this study. Both control and study group patients were of same socio-economic status and dietary habits. Serum zinc and copper level were estimated using standard absorption spectrometer technique and serum selenium by Hydride generation method. We observed significant low serum levels of zinc and selenium while high level of serum copper in carcinoma esophagus patients, as compared with normal healthy controls. This shows an association of serum selenium zinc and copper with cancer esophagus.     

Correlative study of fluoride content in urine,serum and urinary calculi

Fluoride content was measured in 100 urinary stones retrieved by open surgery of stone formers admitted at PGIMS Rohtak and their respective urine and serum and compared with those of healthy individuals. The concentration of fluoride was also measured in the sources of drinking water of these stone formers. The concentration of fluoride was definitely significantly higher in serum (p>0.01) and highly significantly higher in urine (p>−0.001) of stone formers compared to those of healthy individuals. The content of oxalate in serum and 24 h urine of the stone formers was also measured, which was increased significantly (p<0.005 and p<0.001) compared to healthy individuals. The concentration of fluoride was probably significantly higher in drinking water of these stone formers than the normal ones. There was a positive correlation between the content of fluoride of urinary stones and urine of stone patients (r=.88); stone and serum (r=.62); drinking water and stone (r=.85) and their urine and serum (r=.54); urine and drinking water (r=.83) and serum and water (r=.51). These results indicate a definite role of fluoride in urinary stone formation.