茜素黄R与牛血清白蛋白光谱学及分子对接研究

在中性条件下,利用紫外可见分光光谱法和分子对接技术研究了偶氮类小分子染料茜素黄R与牛血清白蛋白的相互作用。紫外可见光谱表明,茜素黄R与牛血清白蛋白键合形成的复合物最大吸收波长为386nm,比茜素黄R红移了13nm,二者的结合摩尔比约为1:1,表观摩尔吸光系数为8.70×103dm3·mol-1·cm-1。结合分子模拟技术研究复合物的结合模式和键合机制,判定茜素黄R在牛血清白蛋白上的结合位置位于site I结合位点,讨论二者之间的作用力以静电作用为主,同时也包括疏水作用和氢键。分子对接模拟结果与实验结论一致。     

牛血清白蛋白荧光猝灭法测定药物中的头孢他美酯

[目的]应用牛血清白蛋白荧光猝灭法建立一种测定药物中头孢他美酯含量的新方法。[方法]牛血清白蛋白（BSA）具有很强的内源荧光性,而头孢他美酯溶液本身不产生荧光。当头孢他美酯与BSA结合后,会导致其荧光强度下降。BSA在λex=342nm处的荧光猝灭程度与头孢他美酯的量在一定浓度范围内呈良好的线性关系,据此建立测定药品中头孢他美酯含量的新方法。[结果]该结合物的最大发射波长为λmax=342nm,与头孢他美酯摩尔浓度在2．07×10^-6-2．58×10—5mol·L^-1范围内线性关系良好。其线性回归方程为△F=1．9684×10^7Ccp＋25．6288,相关系数r=0．9958,检出限为1．01×10^-7mol·L^-1,RSD为0．66％,加标回收率为85．0％-100．99／6。[结论]本方法操作简便、快速,用于实际样本的测定,结果满意。     

专项训练对安静时血清氨基酸水平影响的研究

通过测定普通学生、举重和长泳运动员安静时血清氨基酸发现，有训练者多数氨基酸代谢有较高水平，尤其是与一碳单位代谢和与脂代谢有关的氨基酸水平出现一致性有意义的升高；力量训练者蛋氨酸和赖氨酸水平高于耐力训练者。提示训练和专项训练对安静时血清氨基酸水平均有一定影响。     

大豆皂甙饮料缓解体力疲劳作用的实验研究

目的:研究大豆皂甙饮料的缓解体力疲劳作用。方法:采用雄性ICR小鼠,大豆皂甙饮料按33.3ml/kg.bw、66.7ml/kg.bw、100.0ml/kg.bw连续灌胃30d,测定小鼠负重游泳时间、血乳酸、血清尿素氮和肝糖原含量。结果:100.0ml/kg.bw剂量组小鼠负重游泳时间显著长于对照组(P<0.05).66.Tml/kg.bw剂量组游泳后0min血乳酸含量和3个时点血乳酸曲线下面积显著低于对照组(P<0.05、P<0.01)。33.3ml/kg.bw剂量组肝糖原含量显著高于对照组(P相似文献   

Correlative study of fluoride content in urine,serum and urinary calculi

Fluoride content was measured in 100 urinary stones retrieved by open surgery of stone formers admitted at PGIMS Rohtak and their respective urine and serum and compared with those of healthy individuals. The concentration of fluoride was also measured in the sources of drinking water of these stone formers. The concentration of fluoride was definitely significantly higher in serum (p>0.01) and highly significantly higher in urine (p>−0.001) of stone formers compared to those of healthy individuals. The content of oxalate in serum and 24 h urine of the stone formers was also measured, which was increased significantly (p<0.005 and p<0.001) compared to healthy individuals. The concentration of fluoride was probably significantly higher in drinking water of these stone formers than the normal ones. There was a positive correlation between the content of fluoride of urinary stones and urine of stone patients (r=.88); stone and serum (r=.62); drinking water and stone (r=.85) and their urine and serum (r=.54); urine and drinking water (r=.83) and serum and water (r=.51). These results indicate a definite role of fluoride in urinary stone formation.     

Serum lipids, lipoproteins and sex-hormone binding globulin in breast cancer

Serum levels of various lipid parameters, i.e., triglycerides, total cholesterol, alpha lipoprotein or high density lipoprotein (HDL)-cholesterol and beta lipoprotein or low density lipoprotein (LDL)-cholesterol were measured in a total of 138 histologically proven cases of breast cancer along with 146 control women. The mean levels of serum triglycerides, total cholesterol and LDL-cholesterol were found to be significantly higher in breast cancer cases as compared to controls. However, lower mean levels of serum HDL-cholesterol and sex-hormone binding glubulin (SHBG) were observed in breast cancer patients than the control subjects. The results indicate a probable relation between serum lipids and the activity of sex-hormones. Moreover, the study reflects an overall disturbance of lipid metabolism in the pathological process of breast cancer.     

Correlations between serum and salivary hormonal concentrations in response to resistance exercise

Abstract

The aim of present study was to examine the relationships between serum and salivary values of free testosterone, dehydroepiandrosterone, and cortisol before and after a session of resistance exercise. Twenty-eight healthy men (mean age 40 years, s = 4) participated in the present study. Serum and salivary samples were collected at rest and after a multiple-sets resistance exercise protocol, of approximately 25 minutes duration. Concentrations of free testosterone, dehydroepiandrosterone, and cortisol were measured using radioimmunoassay kits. No significant correlation was observed between serum free testosterone and salivary testosterone (r = 0.22 to 0.26, P > 0.05). Serum cortisol was significantly correlated with salivary cortisol before (r = 0.52, P = 0.005) and after (r = 0.62, P = 0.001) the exercise protocol. Serum and salivary concentrations of dehydroepiandrosterone were significantly correlated before (r = 0.68, P < 0.001) and after (r = 0.7, P < 0.001) exercise. The results of the present study suggest that even under exercise conditions, the salivary values of cortisol and dehydroepiandrosterone can reflect the behaviour of these hormones in blood. However, further studies are necessary to verify if salivary testosterone reflects the behaviour of serum free testosterone during resistance exercise.     

半胱胺对肉鸡部分血清化学参数及酶活性的影响

144只一日龄的AA肉仔鸡随机分为4组(n=36),预饲一周后分别在试验组基础日粮中添加50mg/kg、100mg/kg、150mg/kg的半胱胺,分别于2、4、6周龄采颈静脉血,分离血清,测定四组血清钙、磷和葡萄糖含量及ALT、AST、ALP活性,研究半胱胺对肉鸡部分血清化学参数及酶活性的影响.结果表明,饲料中添加适量的Cs能升高AA肉鸡的血钙水平,而对鸡血清磷、葡萄糖含量没有明显影响;添加50～100mg/kg的Cs对ALT、AST活性没有明显的影响;而饲料中添加Cs对ALP活性有一定影响.     

皖南花猪血清酶的测定和分析

本研究测定了皖南花猪从出生到３６０日龄血清７种酶的水平和活性,并分析了随猪日龄变化各种酶活性的变化规律。结果显示,各种酶随着日龄的变化十分明显,其中３０～６０和１２０～１５０日龄阶段是各种酶变化的转折点：猪去势后这些酶的变化趋势无明显的改变。在后备猪中仅ＡＫＰ、ＧＯＴ和ＣＫ分别与日龄之间的相关达到显著水平（－０．７５３２、－０．７１２１和－０．７５７５）,酶之间的相关仅ＧＯＴ与ＡＫＰ之间表现明显（０．８９９０）;而肥育猪中Ａｍｙ、ＬＤＨ和ＣＫ分别与日龄的相关达到－０．７２９８、－０．９３５７和－０．７６２６,ＧＯＴ分别与ＡＫＰ和Ａｍｙ的相关达到０．７３４９和０．９１１３,ＬＤＨ分别与Ａｍｙ和ＣＫ的相关达到０．７４０３和０．７４８１。     

Effect of Antiresorptive Therapy on Urinary Hydroxyproline in Postmenopausal Osteoporosis

Osteoporosis becomes a serious health threat for older postmenopausal women by predisposing them to an increased risk of fracture. Osteoporosis and associated fractures are an important cause of morbidity and mortality. Special attention is being paid to early detection, management, and treatment of postmenopausal osteoporosis in women. Biochemical markers can enable dynamic and rapid measurement of total body skeletal metabolism and will be clinically useful in the management of postmenopausal osteoporosis women (PMO) and also for assessing the effects of antiresorptive therapy. With this view, we planned to assess osteoclastic activity by determining urinary hydroxyproline in osteoporotic women. The aim of this study is to measure urinary hydroxyproline (expressed as mg of hydroxyproline/g of creatinine) and serum ascorbic acid in postmenopausal women with osteoporosis and without osteoporosis. These biochemical parameters were determined 3 months post antiresorptive therapy (alendronate + calcium + vitamin D) in postmenopausal osteoporosis patients. 60 postmenopausal women with osteoporosis in the age group 45–60 years and 60 healthy postmenopausal women (normal bone mineral density) in the same age group were included in the study. Urinary hydroxyproline levels were significantly increased (P < 0.001) in PMO at baseline level as compared to control group. These levels were decreased significantly (P < 0.001) post therapy in PMO patients. Serum vitamin C levels were significantly decreased (P < 0.001) in PMO patients at baseline level as compared to controls. No significant change occurred of serum vitamin C level post therapy. Raised excretion of hydroxyproline at the baseline level might be due to increased degradation of collagen type I from the bone matrix in osteoporosis. Breakdown of collagen seems to be lowered as reflected by lowering of hydroxyproline excretion post antiresorptive therapy. Alteration in the concentration of this marker can be very well utilized to monitor the effectiveness of therapy. Thus simple, direct urinary assay to measure bone resorption is very useful in monitoring the therapy in PMO and may become an integral part of the management of osteoporosis.