职业病误诊题材与职业医学期刊编辑工作

分析职业病及其误诊的特点.强调职业医学编辑工作中应该重视职业病误诊题材的报道,专业编辑应该加强职业病相关知识的学习,提高识别误诊的能力,将职业病误诊题材纳入选题策划,充实报道的内容.认为通过对职业病误诊的剖析与报道,以引起"疗救者的注意",避免重蹈覆辙,是职业医学期刊编辑的职责.     

Parthenolide inhibits proliferation of vascular smooth muscle cells through induction of G0/G1 phase cell cycle arrest

Objective  This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs). Methods  Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and 30 μmol/L). [³H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism. Results  Treatment with parthenolide significantly decreased the [³H]thymidine incorporation into DNA by 30%∼56% relative to control values in a dose-dependent manner (P<0.05). Addition of parthenolide also increased cell population at G₀/G₁ phase by 19.2%∼65.7% (P<0.05) and decreased cell population at S phase by 50.7%∼84.8% (P<0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression and reduced Cox-2 expression in a time-dependent manner. Conclusion  Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G₀/G₁ cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo. Project (No. 491020-W50315) supported by the Foundation of the Health Bureau of Zhejiang, China     

食管癌组织中Survivin和Ki67的表达及临床意义

目的:观察Survivin和Ki67在食管鳞癌中的表达,并探讨Survivin与Ki67的表达关系及其临床意义.方法:应用免疫组织化学法检测70例食管鳞癌组织石蜡切片Survivin和Ki67的表达,分析Survivin表达与临床病理因素及Ki67的关系.结果:Survivin在食管鳞癌组织中的表达率为72.9%(51/70),Ki67在食管鳞癌组织中的表达率为67.1%(47/70).Survivin表达和Ki67表达与食管鳞癌分化程度、淋巴结转移、TNM分期明显相关(P＜0.05).Survivin表达和Ki67表达呈正相关(P＜0.05).结论:Survivin和Ki67可作为判断食管癌组织恶性程度和预后的指标之一.     

关注高职院校食品安全

食品安全是重要公共安全问题,是高职院校构建和谐校园的重要内容。要加大宣传力度,建立防范食源性疾病的运作机制与预案,进行病理分析,采取相关对策保障学生的健康安全和生命安全。     

Regeneration of Red Cell Cholinesterase Activity Following Pralidoxime (2-PAM) Infusion in First 24 h in Organophosphate Poisoned Patients

Oximes such as pralidoxime chloride reactivate acetylcholinesterase. However their role in management of organophosphate poisoning is controversial. The study was carried out to find effectiveness of pralidoxime chloride (2-PAM) in regenerating red cell acetyl cholinesterase in first 24 h following administration of it in dose recommended by WHO. Eight patients with OPP [chlorpyriphos (3), phorate (3), dichlorvos (1) and monocrotophos (1) who fulfilled the criteria for inclusion were investigated. In addition to decontamination and atropine, all these patients were administered 30 mg/kg body wt of 2-PAM as bolus dose followed by 7.5 mg/kg body wt/h with maximum dose being 500 mg/h as continuous infusion till first 24 h. Red cell AChE activity was estimated every 15 min for first 4 h, one hourly for next 4 h and then 2 hourly till 24 h and subsequently without 2-PAM every 12 h till 7 days or discharge or death which ever earlier. In all the patients maximum increase in activity was observed in first 4 h following which rise was very slow despite continued 2-PAM infusion and reaching a steady state in 20 h in all the cases. The increase in red cell AChE activity observed in diethyl group at 24 h of 2-PAM infusion was 154% vs. 81% in dimethyl group. At 7 days the increase in activity was 215% vs. 118% respectively. However on multiple repeated ANOVA, no statistically significant difference was observed between diethyl and dimethyl groups at admission and discharge (P > 0.05). Similarly no significant difference was observed in three groups when patients were categorized according to WHO classification of organophosphates (P > 0.05). The maximum increase in red cell AChE activity occurs in first 4 h of 2-PAM administration followed by a slow increase despite 2-PAM infusion till 24 h.     

改革细胞生物学实验教学培养学生创新能力

实验教学是细胞生物学课程的重要组成部分．我们在细胞生物学实验中进行了实验教学的探索与改革．通过调整实验教学内容体系：引入辅助教学手段：建立完善的成绩评定体系等几个方面的改革．培养了学生的创新意识兴趣。从而达到提高学生综合素质的目的。     

浅谈医学黑箱理论在植物缺素症诊断中的应用

运用医学的黑箱理论探讨了中医诊断与植物缺素症诊断的相似性,分析了中医诊断以及植物缺素症诊断目前面临的问题,并提出相关建议：中医诊断与植物缺素症诊断应与仪器分析有机结合起来,使中医四诊实现仪器化、客观化和规范化,使植物缺素症诊断更加科学化,以适应现代社会的需要。     

Biochemical Indicator of Sickle Cell Disease: Preliminary Report from India

Blood biochemistry has significant effect on pathophysiology of human body. Recently few studies found the association of biochemical abnormalities in sickle cell patients. Sickle cell disease showed clinical variability where African ancestors have severe phenotype than Indian sicklers. Our aim was to evaluate the biochemicals in sickle cell patients and their effect on severity. Here we present the comparative biochemical levels in sickle cell patients as well as controls. Sickle cell patients diagnosed by HPLC and biochemical analysis done by Beckman-auto analyzer. T test applied for statistical analysis. Result showed the renal abnormality lesser in patients and related biochemical within the normal range and statistically not significant. Electrolytes, hepatic enzymes, alkaline phosphatase and glucose were elevated and statistically significant (P value <0.05). Observation of the study concludes the biochemical abnormality play a significant role in sickle cell patient’s physiopathology and can be used to management of the disease.     

IRS1、Akt、FOXO1在少肌症发生及其运动性缓解中的作用

目的:探索IRS1、Akt、FOXO1在少肌症的发生及运动缓解中的作用。方法:24只雄性SD大鼠分为3组,C组(青年安静组)、S组(40d D-半乳糖注射致衰组)、E组(40d D-半乳糖注射衰老+6wk跑台运动组)。检测C、S、E组大鼠体重、腓肠肌的质量、Phospho-IRS1(Ser307)、IRS1、Phospho-Akt(Ser473)、Akt、核内FOXO1、MAFbx mRNA、MuRF1 mRNA、MyoD mRNA、MyoG mR-NA。结果:与C组相比,S组大鼠腓肠肌/体重降低、Phospho-IRS1(Ser307)升高、IRS1、Phospho-Akt(Ser473)、Akt下降,FOXO1、MAFbx mRNA、MuRF1mRNA、MyoD mRNA、MyoG mRNA升高,与S组相比,E组大鼠上述除MyoD mRNA、MyoG mRNA(均上调)外的指标均发生相反变化。结论:衰老肌肉IRS1/Akt/FOXO1通路的生肌信号抑制、分解信号增强,通路可能通过提高(泛素蛋白酶体途径)分解代谢而非降低(生肌因子途径)合成代谢介导了少肌症发生,长期规律运动引起衰老肌肉IRS1/Akt/FOXO1通路生肌信号增强、分解信号抑制,通路可能通过抑制(泛素蛋白酶体途径)分解代谢及提高(生肌因子途径)合成代谢拮抗少肌症,缓解肌肉流失。     

澳门-北京部分高校教职工心血管疾病危险因素的比较

目的:通过了解和比较澳门-北京两地高校教职工心血管疾病危险因素的差异,为两地教职工心血管疾病的预防提供更具针对性的建议。方法:采用问卷调查和测量140名澳门和460名北京高校教职工生活方式、血脂状况、锻炼习惯等11项心血管疾病的危险因素进行调查和比较。结果:澳门高校教职工心血管疾病危险因素的平均数目显著低于北京高校教职工,在高甘油三酯、高血压、吸烟等指标方面表现优于北京高校教职工。两地教职工都存在肥胖和运动参与不足的问题,且澳门经常运动者比例显著低于北京高校教职员。另外,无论北京或澳门,男性高校教职员心血管疾病危险因素数目均显著高于女性。结论:北京和澳门高校教职工都应更多地参与运动,减少肥胖的发生,降低心血管疾病的危险因素,男性教职工更应关注自身健康。