FABP2 gene polymorphism and metabolic syndrome in elderly people of croatian descent

Introduction:

Metabolic syndrome (MetS) is a multifactorial disorder in which dyslipidemia plays an important role. Fatty acid-binding protein 2 (FABP 2) is responsible for transport of free fatty acids in the intestinal endothelium cells. FABP2-genetic variants might affect plasma lipid concentrations and intracellular lipid transport. The aim of this study was to investigate the association between FABP2 Ala54Thr genetic polymorphism and metabolic syndrome and some biochemical and anthropological parameters in elderly subjects.

Materials and methods:

This cross-sectional study included 140 men and 176 women older than 70 years. Fasting serum concentration of glucose, lipid parameters, total proteins and C-reactive protein were determined by standardized methods. Presence (MetS(+)) or absence (MetS(−)) of MetS was determined according to criteria of the International Diabetes Federation. FABP2 genetic polymorphism Ala54Thr (rs1799883) was genotyped with PCR-RFPL.

Results:

The genotype frequencies for Ala/Ala, Ala/Thr and Thr/Thr genotype were 60, 36 and 6 in MetS(−), and 131, 70 and 13 in MetS(+), respectively, without statistical significance (P = 0.567). Ala/Ala genotype was a subgroup of non-carriers, while Ala/Thr and Thr/Thr genotypes were Thr54-carriers. Median triglyceride concentration was significantly lower in carriers then in non-carriers for whole MetS(+) group (P = 0.050); there were no significant difference between men with MetS (P = 0.144), but there was a difference between women with MetS (P = 0.020). T-test showed that mean HDL cholesterol concentrations in MetS(+) group for Thr54-carriers was significantly higher in whole group (P = 0.001), and for both genders (men P = 0.039; women P = 0.004) as compared to non-carriers.

Conclusions:

FABP2 genetic polymorphism is associated with lower triglyceride and higher HDL-cholesterol concentrations in elderly subjects with MetS. This genetic variation might be a useful marker for understanding dyslipidemia in MetS.     

面向21世纪的运动医学研究(综述)

2 0世纪生物技术与生命科学发生了划时代的突破与历史性的变革 ,其科技进步震撼了人类社会。 2 1世纪 ,随着人类基因组计划的完成 ,后基因组计划的全面展开 ,以基因工程为主导的生物技术将影响 1个国家的经济前途 ,并以巨大的活力推动社会生产力的飞速发展。作为运动医学工作者 ,在以基因工程为主导的生物技术迅猛发展的今天 ,用此技术研究体育运动问题 ,责无旁贷。在此 ,作者将就近百年来运动医学研究作一回顾 ,并对未来热点问题和前沿研究作一展望     

C++中运算符重载的应用探讨

多态性是面向对象程序设计的重要支柱之一,而运算符重载又是实现多态性的一个重要途径。运算符重载使得程序员把运算符的使用扩展到操作数是对象的情况,由运算符构成的表达式常常比函数调用更简洁易懂。本文对C 中运算符重载的概念及规则做了概括,对重定义运算符做了说明。     

Study of apolipoprotein E genetic polymorphism in patients with atherosclerotic cerebral infarction

Objective: To explore the frequency and significance of ApoE gene polymorphisms in Chinese patients with atherosclerotic cerebral infarction (ACI). Methods: Polymerase chain reaction and gene sequencing, single nucleotide polymorphisms of ApoE gene were used to analyze 33 cases of patients with ACI and 35 controls. Results: The frequencies of ApoE gene single nucleotide polymorphisms 465C/G, 462C/G and 451delC in the ACI group were significantly higher than those in the control group (P<0.05). The prevalence of polymorphism 486G/T in the control group was significantly higher than that in the ACI group (P=0.011). Conclusions: 465C/G,462C/G and 451delC polymorphisms might be associated with ACI.486GT allele might have protective effect on the pathogenesis of ACI.     

食蟹猴疟原虫子孢子多态性研究

采用猴1－蚊－猴。循环感染的方法，对疟原虫子孢子多态性学说进行验证．结果发现．接种了食蟹猴疟原虫速发型虫体子孢子的名只猕猴，在接种后第8天全部出现速发．除一只死之外其余猴也全部出现复发；接种了食蟹猴疟原虫迟发到虫体于孢子的2只猕猴也都出现速发和复发．因此．用子孢子多态性学说很难解释食蟹猴疟原虫的复发．食蟹猴疟原虫不具有两种类型的子孢子．     

病毒中的多态性研究与防范

病毒多态技术是当今病毒技术研究的热点和难点。将多态技术应用于病毒中,可以使病毒特征码不固定或者没有特征码,给病毒查杀带来很大的困难。本文在深入分析和研究了病毒多态的原理后,提出了多态引擎的关键技术,为查杀各种变种病毒提供了理论依据。     

Study of apolipoproteinc3 Sstl polymorphism in healthy volunteers from Northern India

Several studies including a small case-control (hypertriglyceridemic/normotriglyceridemic individuals) study by us revealed close association between rare S2 allele ofAPOC3 Sstl polymorphism and hypertriglyceridemia. With the understanding that Asian Indians are highly vulnerable to the adverse effects of hypertriglyceridemia, we extended the investigation and studied the frequency distribution of this polymorphism in 216 healthy volunteers from Northern plains of India. We found that more than 50% of the study population had one or two S2 allele. This may suggest that a larger fraction of this population is genetically predisposed to hypertriglyceridemia.     

Predisposition of genetic polymorphism with the risk of urolithiasis

Urolithiasis is a relevant clinical problem with a subsequent burden for health system. The aim of this review is to provide recent progress made using genetic polymorphisms to define pathophysiology, to identify persons at risk for kidney stone disease and to predict treatment response. Population case-control studies are useful both as an alternative and an adjunct as compared to family studies. These involve either whole genome scanning or candidate gene approaches. While whole genome scanning is likely to be widely used in future, at present, candidate gene studies are more feasible. When performing candidate gene case-control studies factors such as study design, methods for recruitment of case and controls, selection of candidate genes, functional significance of polymorphisms chosen for study and statistical analysis require close attention to ensure that only genuine associations are detected. Some of the significant genes that play role in stone formation include calcitonin receptor gene (CTR), vitamin D receptor (VDR), Urokinase, Interleukin, (IL-1β, IL-Ra), E-Cadherin, Androgen & oestrogen receptor gene, vascular endothelial growth factor (VEGF) and Arginine p21. In our case-control study we studied CTR, VDR, Urokinase, IL-1β(−511 and +3954), IL-Ra from north India and predict that VDR, IL-β (-511) and IL-1Ra gene may be used as a possible genetic marker for earlier detection in patients who are at risk for calcium oxalate stone disease. Further, linkage disequilibrium and haplotype structure of a certain candidate gene is important for association analysis. When a certain polymorphic allele has been found to be associated with disease, it is further explained on basis of LD and haplotype structure by one or more other alleles. Once it is determined which haplotype carries the risk allele, by means of molecular biological functional analyses, the variants on that haplotype allele truly causing the effect can be determined.     

Leucine125Valine (Leu125Val) Gene Polymorphism of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) and Myocardial Infarction in Indian Population

Platelet-endothelial cell adhesion molecule-1 (PECAM-1) has role in atherosclerotic plaque development as well as in thrombosis leading to myocardial infarction (MI). Present study was aimed to analyse the association of PECAM-1 Leu125Val gene polymorphism with MI in Indian population. Subjects included healthy individuals as control (N = 116) and MI patients (N = 100) divided into two groups; MI patients at presentation of the acute event (MI-Group-1, N = 46) and patients with recent event of MI stabilized with treatment 4.5 days from their symptoms (MI-Group-2, N = 54). The difference in the distribution of Leu125Val genotype frequencies of controls and patients did not reach statistical significance. However Leu allele frequency (0.57) was more associated with MI patients as compared to control (0.504). sPECAM-1 levels were significantly elevated in patients at acute event of MI (MI-Group-1) by 44.1% (P = 0.009) as compared to controls and by 95.2% (P = 0.001) as compared to stabilized MI patients (MI-Group-2).     

从HEPC 基因筛选预测HiHiLo 训练对左心室结构与功能影响的 分子标记

通过对HEPC基因SNP/A2032G 与HiHiLo 训练后左心室结构与功能指标变化的关联分析,从HEPC 基因中筛选预测HiHiLo 训练效果的分子标 记。方法：对66 名平原地区居住的北方汉族男子进行为期30 天的HiHiLo 训练,测定其训练前后安静状态和递增负荷运动下的左心室结构与功能指 标。应用PCR- RFLP 技术解析受试者SNP/A2032G 多态性,采用关联方法分析SNP/A2032G 与训练前后左心室结构功能指标变化的关联性。结果：（1）HiHiLo 训练后,受试者ESD、EDD、PWD、IVSD、LVM和LVMI 等6 项心室结构指标均增长;心室功能指标中HR、CO、COI 下降,而SV、SVI 和EF 增长。（2）HiHiLo 训练后,AA型PWD和IVSD的增加量显著高于AG型和GG型,AA型100W/HR 的下降量显著高于GG型,AA型Re/SV 和Re/SVI 的增加量显著高于AG型和GG型。结论：HEPC基因SNP/A2032G 的AA型可以作为预测HiHiLo 训练后心室结构和功能指标训练效果的分子标记。