Up-regulation of mitochondrial antioxidation signals in ovarian cancer cells with aggressive biologic behavior

Objective  

Recently, a high frequency of mutations in mitochondrial DNA (mtDNA) has been detected in ovarian cancer. To explore the alterations of proteins in mitochondria in ovarian cancer, a pair of human ovarian carcinoma cell lines (SKOV3/SKOV3.ip1) with different metastatic potentials was examined.     

三元场对小白鼠抗衰老的作用

为了探索人类抗衰老，长寿的奥秘，探讨了元极堂内三元场对防病，治病，延缓衰老的生物学效应，选用小白鼠作为动物模型，用现代生物学，免疫学，生物化学的技术研究三元场对小白鼠抗衰老作用影响，对小白鼠超氧化物歧化酶活性的影响、对小白鼠氧自由基的影响，并进行了小白鼠抗疲劳试验，耐压缺氧试验，耐高温试验、结果表明三元场对机体防病，治病，延缓衰老具有明显作用。     

人超氧化物歧化酶（SOD）基因的原核表达与蛋白纯化

从人直肠癌细胞株Colo320中提取总RNA,经RT-PCR扩增出SOD基因片段,经限制性内切酶（BamHI,Sinai）酶切后按正确的读码框顺序插入到pGEX-4T-2表达载体上,重组质粒转化大肠杆菌,经菌落PCR和质粒双酶切鉴定、序列测定确认,证实成功地构建了人SOD基因融合表达载体．转化菌经IPTG诱导表达,SDS-PAGE显示有与预期大小约44kD相吻合的融合蛋白带,获得了可溶性表达的目的蛋白,采用Glutathione Sepha—rose4B亲和层析对重组蛋白进行纯化,获得了纯度很高的目的蛋白．     

肉鸡肺动脉高压肝脏和心脏线粒体氧自由基变化研究

400只艾维茵肉鸡按常规方法育雏在21日龄时随机分为两组,A组200只在24℃下饲养,B组200只在低温12℃下饲养。在28、35、42、49日龄测定腹水心脏指数和红细胞压积,分离鉴定腹水肉鸡心、肝线粒体,测定其所含的MnSOD活性以及线粒体膜结构的MDA含量变化经。结果表明:(1)低温处理后一周和二周,在肝脏和心脏线粒体中,SOD含量升高或显著升高(p<0.05)。MDA含量降低或显著降低(p<0.05)。(2)低温处理三周后,SOD的含量降低或显著降低。MDA的含量升高或显著升高(p<0.05)。本实验结果显示肝脏和心脏的线粒体SOD和MDA的变化与肉鸡腹水综合征的发生发展关系密切,氧自由基在亚细胞水平上参与了疾病的病理过程。     

Effect of Vitamin E Supplementation on Biochemical Parameters in Pesticides Sprayers of Grape Gardens of Western Maharashtra (India)

The aim of this study was to see the biochemical effects of pesticides on sprayers of grape gardens before and after 15 days of vitamin E supplementations in Western Maharashtra (India), who were occupationally exposed to various pesticides over a long period of time (about 5 to 15 years). Blood samples were collected from all study group subjects for biochemical parameters assays before and after 15 days of vitamin E supplementation. Sprayers of grape gardens were given 400 mg of vitamin E tablet/day for 15 days. After 15 days of vitamin E supplementation to sprayers of grape gardens, we observed significantly decreased aspartate transaminase (10.88 %, P < 0.05, r = 0.88), alanine transaminase (25.92 %, P < 0.01, r = 0.46) and total proteins (3.32 %, P < 0.01, r = 0.33), whereas, no statistically significant change was found in serum acetyl cholinesterase, C-reactive proteins, albumin (ALB), globulins and ALB/globulin ratio as compared to before vitamin E supplementation. Sprayers of grape gardens, who received vitamin E supplementation, showed significantly decreased serum lipid peroxide (LP) (18.75 %, P < 0.001, r = 0.63) and significantly increased RBC-superoxide dismutase (SOD) (12.88 %, P < 0.001, r = 0.85), RBC-Catalase (CAT) (24.49 %, P < 0.001, r = 0.70), plasma ceruloplasmin (CP) (4.6 %, P < 0.01, r = 0.80), serum zinc (4.57 %, P < 0.01, r = 0.83) and serum copper (4.37 %, P < 0.01, r = 0.79) as compared to values before vitamin E supplementation. These results showed that vitamin E supplementation has ameliorating effects on these transaminase enzymes, suggesting that it may have a protective effect on liver, from pesticides induced damage. In this study vitamin E supplementation might have decreased LP levels by breaking chain reaction of lipid peroxidation. Present results indicate that vitamin E plays a crucial role in restoring the antioxidant enzymes such as SOD, CAT and CP, in population exposed to pesticides. This helps to enhance its antioxidant ability. Therefore, it is suggested that farmers, pesticide applicators, workers in the pesticide industry and other pesticide users, who come in regular contact with pesticides, may be benefited by supplementation with vitamin E.     

Assessment of Oxidative Stress and Inflammatory Process in Patients of Multiple Myeloma

Multiple myeloma is a disseminated malignancy of monoclonal plasma cells that accounts for 15 % of all hematological cancers. The present study was conducted to evaluate the role of inflammation and oxidant-antioxidant dynamics in the etiology of this disease. The study population comprised of 20 cases of multiple myeloma and 20 healthy controls. The parameters evaluated were serum malondialdehyde (MDA), superoxide dismutase (SOD) and ferritin levels. The serum MDA levels were 1.9 ± 0.96 nmol/ml in cases as compared to 0.98 ± 0.55 nmol/ml in the controls. Similarly, a statistically significant difference was noted in the SOD and ferritin levels between the cases and controls (93.2 ± 23.8 vs. 210.1 ± 190.5 U/ml and 285.8 ± 216.4 vs. 131.8 ± 30.1 ng/ml respectively). Our study highlights the imbalance in the oxidant-anti oxidant mechanism and the role of smoldering inflammation in the etiology of multiple myeloma.     

力竭运动恢复期大鼠血液中自由基的研究

力竭运动后24小时大鼠血液中LPO、SOD、GSH-px的恢复结果表明,力竭运动后即刻半小时,血浆LPO含量和SOD活性不断增高,3小时左右达到最高值,随后不断降低,运动后24小时基本恢复到静息状态。力竭运动后即刻血液中GSH-px有显著升高,随后一直都呈现下降趋势,表明LPO、SOD具有相似的变化趋势,而GSH-px则与它们的变化不一致。     

限制肌血流量中等强度负荷训练对速度滑冰运动员骨骼肌抗氧化能力的影响

采用限制肌血流量运动负荷训练的方法,观察训练前后速度滑冰运动员血浆中超氧化物歧化酶、谷胱甘肽过氧化物酶、总抗氧化能力的变化,以探讨限制肌血流量中等强度负荷训练对骨骼肌抗氧化能力的影响。     

Comparison of lipid profile and antioxidant enzymes among south Indian men consuming coconut oil and sunflower oil

In this study, we compared the lipid profile and antioxidant enzymes of normal and diabetic subjects consuming two different types of oil as cooking medium. 70 normal, healthy subjects were taken as controls and 70 subjects with Type 2 diabetes were recruited in patient group. Each group was further subdivided into two subgroups of 35 subjects each, consuming coconut oil and sunflower oil respectively as cooking medium. Samples of blood were collected and analyzed for serum total cholesterol, triacylglycerols, and cholesterol in lipoprotein fractions. Total glutathione and glutathione peroxidase were measured in erythrocytes and superoxide dismutase in serum. Triacylglycerols, LDL and VLDL cholesterol levels were high in the diabetic subjects compared to the controls. Total glutathione and glutathione peroxidase values showed significant decrease in diabetic subjects as compared to the controls, while superoxide dismutase values showed significant difference between coconut oil consuming groups. Though lipid profile parameters and oxidative stress were high in Type 2 diabetic subjects compared to controls, no pronounced changes for these parameters were observed between the subgroups (coconut oil vs. sunflower oil).     

不同运动强度对血浆氧自由基代谢水平的研究

用化学Ｊａｇｉ法和邻苯三酚法观察了三种不同强度运动前后血脂质过氧化物分解产物丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）的改变,以探讨运动强度与氧自由基（ＯＦＲ）的代谢水平,对其研究其有重要理论价值,并对多因互中评价运动员身体机能状态变化、运动强度、运动训练适应水平以及运动性疲劳发生提供有益的资料。