高温环境下大鼠一次性有氧运动后血IL-2、SOD、MDA、SOD/MDA指标的变化研究

方法:从42只清洁级雄性SD大鼠中随机选取36只分为对照组(A组)、热应激组(B组)、有氧运动组(C组)与运动+热应激组(D组),剩余6只用于测定乳酸阈强度。各组实验后即刻测定血清IL-2、SOD、MDA等水平。结果:B、D组大鼠经热暴露后肛温均呈非常显著性升高,且出现典型的热应激症候。B组血清IL-2、SOD、SOD/MDA均显著低于A组,C组显著高于A组C组MDA显著低于A组;D组血清IL-2、SOD、SOD/MDA均显著低于A组(P<0.01)、B组(P<0.05)与C组(P<0.01),MDA显著高于A组(P<0.01)、B组(P<0.05)与C组(P<0.01)。结论:运动热应激可引起自由基生成增多,加重组织细胞膜的结构和功能损害,造成调节温度的信号通路发生中断,体温失衡;之后可能通过调节神经-内分泌-免疫系统机能,抑制IL-2的生成。IL-2可作为评定运动热应激过程中免疫机能的重要指标。     

银杏叶提取物抗超氧自由基活性研究

研究了连苯三酚产生超氧自由基体系的条件,并在此体系下测定了银杏叶提取物的抗超氧自由基的作用。实验表明,提取物能抗超氧自由基,抑制率最高达23.1%。本方法操作简便,测定快速,是一种简便的筛选抗超氧自由基清除剂和有效抗氧化药物的方法。     

Toxicology and free radicals scavenging property of Tamra bhasma

Free radicals are implicated in various chronic diseases. There has always been a search for new antioxidants. In this paper we have investigated Tamra bhasma, a metallic ayurvedic preparation. It is a time-tested medicine in Ayurveda and is in clinical use for various ailments specifically the free radical mediated diseases. Our results show that Tamra bhasma inhibits lipid peroxidation (LPO), prevents the rate of aerial oxidation of reduced glutathione (GSH) content and induces the activity of superoxide dismutase (SOD) in rat liver homogenate in the bi-phasic manner. The drug was orally given for 7, 15 and 30 days in different doses. Best protective response was found at the dose of 0.5mg/100g body weight in albino rats, although it showed some histopathological changes at the dose of 20mg/100g body weight. The results suggest that this Ayurvedic preparation is not merely a source of copper metal, but it is a strong anti-oxidant with no detectable adverse effect in lower doses of therapeutic range.     

三元场对小白鼠SOD活性影响的研究

超氧化物歧化酶(SOD)广泛存在于各种生物体内,是迄今为止发现的唯一以氧自由基为底物的酶,SOD是生物体内一种重要的氧自由基清除剂,对生物起防护作用,具有抗衰老、抗肿瘤、抗免疫性疾病和辐射防护的作用,通过化学法测定SOD,以研究元极堂内三元场对小鼠SOD活性的影响,探讨防病治病延缓衰老作用的机理,测试结果表明:小白鼠肝、脑SOD活性均提高近1倍,证实元极堂内三元场具有防治疾病等作用。     

Lipid peroxidation in erythrocytes of patients with type 2 diabetes mellitus

Lipid peroxidation by measurement of Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant status by determining the activities of the enzymes, Catalase (Cat), Glutathione Peroxidase (GPx) and Superoxide Dismutase (SOD) and the level of Reduced Glutathione (GSH) in the erythrocytes of patients with type 2 Diabetes Mellitus were investigated. It was observed that the level of TBARS in the erythrocytes was increased by 50% showing a significant generation of free radicals in the erythrocytes of these patients. The activities of both Cat and SOD were enhanced while that of GPx was not altered. The level of GSH was also not changed.     

Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties

A relationship between status epilepticus (SE) and oxidative stress has recently begun to be recognized. To explore whether the flavonoids extracted from licorice (LFs) have any protective effect on kainate (KA)-induced seizure in mice, we treated mice with LFs before and after KA injection. In KA-treated mice, we found that superoxide dismutase (SOD) activity decreased immediately after the onset of seizure at 1 h and then increased at 6 h. It returned to baseline 1 d after seizure and then increased again at 3, 7, and 28 d, while malondialdehyde (MDA) content remained at a high level at 1 h, 6 h, 3 d, 7 d, and 28 d, indicating a more oxidized status related to the presence of more reactive oxygen species (ROS). Treatment with LFs before KA injection reversed the seizure-induced change in SOD activity and MDA content at 1 h, 6 h, 3 d, 7 d, and 28 d. Treatment with LFs after seizure decreased KA-induced SOD activity and MDA content at 7 and 28 d. Also, LF pre- and post-KA treatments decreased seizure-induced neuronal cell death. Subsequently, Morris water maze tests revealed that the escape latency was significantly decreased and the number of target quadrant crossings was markedly increased in the LF-treated groups. Thus, our data indicate that LFs have protective effects on seizure-induced neuronal cell death and cognitive impairment through their anti-oxidative effects.     

Oxygen free radical modified DNA: Implications in the etiopathogenesis of Systemic lupus erythematosus

The present study was designed to probe the possible role of singlet oxygen and superoxide anion radical modified DNA in the etiopathogenesis of Systemic lupus erythematosus. These species were generated by the exposure of riboflavin to 365 nm UV light. Modified DNA showed single strand breaks, hyperchromicity at 260nm and decrease in Tm. The modified DNA induced high titer antibodies in experimental animals. The antibodies showed reactivity with various nucleic acid polymers, a property commonly associated with Systemic lupus erythematosus anti-DNA autoantibodies. Systemic lupus erythematosus sera showed preferential binding of modified DNA over native DNA in direct binding and competitive binding solid phase immunoassays and band shift assays. The results suggest for the possible involvement of the singlet- superoxide modified DNA as a potential trigger for anti- DNA autoantibody production in SLE and thus in the etiopathogenesis of the disease.     

黄参多糖对小鼠骨骼肌自由基代谢及超微结构的影响

通过超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量和超微结构的变化研究了黄参多糖（SGP）对小鼠骨骼肌自由基代谢的影响。数据显示,游泳＋SGP各剂量组安静时SOD活性是对照组的两倍左右,力竭运动后即刻组比对照和单纯运动的都高,且差异显著,24h后恢复也快,并有明显的量效关系,以剂量150—200mg·kg^-1最佳。同时超微结构还表现出,对照组力竭运动后由于自由基的攻击出现了线粒体膜损坏,甚至有空泡化。还有肌丝松散、Z带弯曲等病理现象的产生。而灌服SGP的各组小鼠无这类现象,并有肌糖原积累、线粒体增多的证实。这全面显现出SGP对清除自由基,保护细胞膜,延缓疲劳,提高运动能力的作用。     

拟黑多刺蚁对小鼠SOD、MAO含量和脂褐素的影响

本文观察了拟黑多刺蚁匀浆液对小鼠血中SOD活性、MAO含量及脾脏脂褐素的影响。给药组老龄小鼠与老年对照组比较RBC-SOD增高(P<0.01),脾脏脂褐素分布相对减少,而血浆MAO水平显著降低(P<0.01);与成年对照比较二酶均未见显著差异。     

磁场对绿豆芽长及超氧化物歧化酶的实验研究

用恒定强度的磁场(1T)对绿豆进行不同时间的处理,得出了不同磁场处理时间和培养时间对绿豆芽长和超氧化物歧化酶(SOD)活性的影响。实验结果表明:恒定磁场强度下不同处理时间对绿豆的芽长和SOD活性是有影响的,磁场强度为1T时,在6个处理时间中,30 min处理组在每次测量时芽最长。对绿豆的SOD测定表明:经过磁场处理后的绿豆SOD含量产生了明显的差异,培养时间为7 h的实验组在各磁场处理时间下的SOD活性均高于对照组,在培养21 h后差异最大;磁处理具有滞后效应,磁处理短期内并不产生明显的效果;豆芽SOD含量和芽长并不成对应关系。