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101.
以Web of Science的艾滋病疫苗研究文献作为数据来源,利用引文分析可视化软件HistCite,找出代表艾滋病疫苗研究领域发展的重要文献,并分析文献之间引用与被引用关系和引用序列,获得引文编年图谱,梳理出艾滋病疫苗研究领域的发展规律和历史发展轨迹,为国内外相关领域的研究人员提供一定的参考。 相似文献
102.
Stephanie Fulton Donna S. Gibson Jan Orick Ann Marie Clark 《Medical reference services quarterly》2019,38(1):87-96
The primary goal of this project is to understand how each National Cancer Institute-designated cancer center library, and all libraries that support cancer research, function within their institutions. Through an in-depth survey focused on three major areas (staff, content and tools procurement, and user services), the research team hopes to determine how a cancer-centric library can be successful in supporting quality patient care, research excellence, and education. Additionally, the survey will examine the necessary minimum staffing levels for librarians and information professionals based on organizational size and degree of research focus. The survey will seek out the new skills librarians will need to deliver optimal services. The survey will also explore how content libraries purchase reflects and maps to constituents’ current medical and research activities. Libraries within a research intense environment have a responsibility to align with researchers and health care professionals to provide resources and services that support their workflows. Cancer libraries need to be attuned to their institutions’ missions, whether that includes excellent patient care, research endeavors, or cutting-edge educational programs. The information gathered from the survey will provide data for this research team to define the vision and standards of excellence for a cancer specialized research library. 相似文献
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VATS,THE PAST Historical perspective The application of thoracoscopy can be traced back to nearly one hundred years ago,when Dr.Jacobaeus(1910)first reported his experiences in the diagnosis and treatment of pleural effusions by tho-racoscope in1909.Most patients who needed to un-dergo thoracoscopy at that time suffered from pul-monary tuberculosis(TB)(“the era of enthusiasm”)(Cutler,1933),and was rarely performed after the development of chemotherapy for patients with TB after the195… 相似文献
105.
不同级别宫颈上皮内瘤变及宫颈癌患者HPV病毒感染情况研究 总被引:1,自引:0,他引:1
目的探讨各级别宫颈上皮内瘤变及宫颈癌患者HPV病毒感染情况。方法本研究采用TCT联合荧光定量PCR法检测HPV病毒,病理学检查为金标准,TCT筛查正常者为对照组。结果HPV病毒感染率随年龄增加有下降的趋势。HPV-16、58、18三种亚型位居各级别宫颈病变HPV病毒感染的前三位。HPV病毒双重或多重感染与宫颈病变级别之间无正相关性。结论HPV-16、58、18三种亚型致病力强,易导致高级别宫颈病变,具有较高的致癌性。 相似文献
106.
Preoperatively molecular staging with CM10 ProteinChip and SELDI-TOF-MS for colorectal cancer patients 总被引:8,自引:0,他引:8
Xu WH Chen YD Hu Y Yu JK Wu XG Jiang TJ Zheng S Zhang SZ 《Journal of Zhejiang University. Science. B》2006,7(3):235-240
Objectives: To detect the serum proteomic patterns by using SELDI-TOF-MS (surface enhanced laser desorption/ ionization-time of flight-mass spectrometry) technology and CM10 ProteinChip in colorectal cancer (CRC) patients, and to evaluate the significance of the proteomic patterns in the tumour staging of colorectal cancer. Methods: SELDI-TOF-MS and CM10 ProteinChip were used to detect the serum proteomic patterns of 76 patients with colorectal cancer, among them, 10 Stage Ⅰ, 19 Stage Ⅱ, 16 Stage Ⅲ and 31 Stage Ⅳ samples. Different stage models were developed and validated by support vector machines, disctiminant analysis and time-sequence analysis. Results: The Model Ⅰ formed by 6 protein peaks (m/z: 2759.58, 2964.66, 2048.01, 4795.90, 4139.77 and 37761.60) could be used to distinguish local CRC patients (Stage Ⅰ and Stage Ⅱ) from regional CRC patients (Stage Ⅲ) with an accuracy of 86.67% (39/45). The Model Ⅱ formed by 3 protein peaks (m/z: 6885.30, 2058.32 and 8567,75) could be used to distinguish locoregional CRC patients (Stage Ⅰ, Stage Ⅱ and Stage Ⅲ) from systematic CRC patients (Stage IV) With an accuracy of 75.00% (57/76). The Model Ⅲ could distinguish Stage Ⅰ from Stage Ⅱ with an accuracy of 86.21% (25/29). The Model Ⅳ could distinguish Stage Ⅰ from Stage Ⅲ with accuracy of 84.62% (22/26). The Model Ⅴ could distinguish Stage Ⅱ from Stage Ⅲ with accuracy of 85.71% (30/35). The Model Ⅵ could distinguish Stage Ⅱ from Stage Ⅳ with accuracy of 80.00% (40/50). The Model Ⅶ could distinguish Stage Ⅲ from Stage Ⅳ with accuracy of 78.72% (37/47). Different stage groups could be distinguished by the two-dimensional scattered spots figure obviously. Conclusion: This method showed great success in preoperatively determining the colorectal cancer stage of patients. 相似文献
107.
BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001 相似文献
108.
Rohit Kumar Gupta Sagar Jayantilal Dholariya Smita Kaushik S. K. Gupta Reva Tripathi Shyam Lata Jain 《Indian journal of clinical biochemistry : IJCB》2021,36(2):221
Ovarian cancer has been emerged as a most common and lethal gynecological malignancy in India. High serum insulin and low adiponectin have been associated with increased risk of ovarian cancer. But their role in development of ovarian cancer is conflicting and little evidence is available. We aimed to evaluate blood levels of insulin and adiponectin in epithelial ovarian cancer (EOC) patients and their association with the risk to develop EOC. The study included following three groups; Group 1: fifty cases of cytohistopathologically confirmed cases of EOC, Group 2: fifty age matched cases of benign ovarian conditions and Group 3: fifty ages matched healthy controls with no evidence of any benign or malignant ovarian pathology as ruled out by clinical examination and relevant investigations. Cytohistopathologically confirmed and newly diagnosed cases of EOC and benign ovarian cancer were included in this study. The median value of fasting serum insulin was significantly high (15.0 µlU/ml, P = 0.02) and adiponectin were significantly low (5.1 µg/ml, P < 0.001) in ovarian cancer patients compared to benign ovarian tumors and healthy controls group. A significant increase risk of ovarian cancer was found in high tertile (≥ 18.7 µlU/ml) of serum insulin level (OR = 2.7; 95% CI = 1.00–6.67, P = 0.04) and lower tertile (≤ 5.45 µg/ml) of adiponectin level (OR = 3.2; 95% CI = 1.10–9.71, P = 0.03). High serum insulin level and low adiponectin levels were significantly associated with increased risk for development of ovarian cancer. 相似文献
109.
M. Smita K. Naidu A. N. Suryakar Sanjay C. Swami R. V. Katkam K. M. Kumbar 《Indian journal of clinical biochemistry : IJCB》2007,22(2):140-144
Cervical cancer (CaCx) is a global public health problem as it is the second most common cancer leading to the death of women
worldwide. Many references revealed that the low levels of antioxidants induce the generation of free radicals leading to
DNA damage and further mutations. In the present study attempt have been made to evaluate the levels of serum Lipid peroxide,
Nitric Oxide (NO.) Erythrocytic—Superoxide Dismutase (RBC-SOD), Vitamin-C, serum Copper (Cu) and serum Zinc (Zn). 120 patients were divided
in 4 groups according to the increasing CaCx stages i.e. stage I, II, III & IV respectively. All the patients were around
the age group of 25–65 years. 30 healthy women between the same age group were treated as controls. Highly significant increased
values of MDA, NO. and Cu were observed (p<0.001) whereas the activity of RBC-SOD, levels of Vitamin-C and Zn were significantly decreased in
CaCx patients as compared with healthy controls (p<0.001). Cu/Zn ratio was found to be altered in CaCx patients. From our
findings it can be concluded that the oxidative stress is induced among CaCx patients, which inturn increases the risk of
CaCx. 相似文献
110.
文章针对第二类疫苗的特殊性,总结以往招标采购的经验与教训,对第二类疫苗招标采购的质量保证、价格优惠、供货商服务良好、操作方便、供货稳定等进一步加强规范化,提出建议. 相似文献