高效液相色谱在生化药物分析中的应用与展望

高效液相色谱因为其独特的优势,在药物分析上有着巨大的作用。文章综述了近年来国内外学者对于高效液相色谱在生化药物分析中的应用进展,简述了一些新的方法和新的应用。     

《生物药物仪器分析》学习领域课程课业设计的探索

学习领域课程以典型工作任务为载体,其学习内容的组织形式是课业。课业是学习情境的＂物质化＂表现,课业设计在教师作用、学生地位等方面与传统实践教学有明显区别,《生物药物仪器分析》课程对此进行了实践探索。     

On the Performance of Semi- and Nonparametric Item Response Functions in Computer Adaptive Tests

Large-scale assessments often use a computer adaptive test (CAT) for selection of items and for scoring respondents. Such tests often assume a parametric form for the relationship between item responses and the underlying construct. Although semi- and nonparametric response functions could be used, there is scant research on their performance in a CAT. In this work, we compare parametric response functions versus those estimated using kernel smoothing and a logistic function of a monotonic polynomial. Monotonic polynomial items can be used with traditional CAT item selection algorithms that use analytical derivatives. We compared these approaches in CAT simulations with a variety of item selection algorithms. Our simulations also varied the features of the calibration and item pool: sample size, the presence of missing data, and the percentage of nonstandard items. In general, the results support the use of semi- and nonparametric item response functions in a CAT.     

汽车变速器CAE仿真与试验分析

变速器作为汽车动力总成系统的关键部件,其功能的可靠、耐久的长短等性能直接影响到汽车的整体功能。CAE仿真与试验是变速箱性能验证的重要手段,通过结合设计阶段的CAE仿真与样件阶段的试验验证进行对比研究,能够使变速器在量产前完善设计,减少试验的样本量,从而实现缩短变速器设计周期和降低成本的目的。     

对《食品理化检验》实验教学改革的几点思考

《食品理化检验》是食品科学与工程专业一门实验性和应用性很强的专业基础课。但目前的实验教学中存在很多问题,如实验内容孤立、学生缺乏实际操作训练、样品分析中只重视测定环节等。为此,提出了进行教学改革的几点思考:让学生参与实验设计,充分发挥学生的主动性和创造性;让学生参与实验前期准备工作,有利于加强学生基本操作技能的训练;让学生自己进行样品制备,加强学生在样品的采集、制备与预处理、样品的保存等方面能力的训练;重视实验数据处理和实验报告的撰写。     

Evaluation of adverse drug reaction formatting in drug information mobile phone applications

Objective:To evaluate the differences in presentation (formatting) of adverse drug reaction (ADR) information within drug monographs in commonly used drug information (DI) mobile device applications.Methods:A cross-sectional analysis of ADR formatting of twenty commonly prescribed oral medications within seven DI mobile applications was performed. Databases were assessed for ADR information, including presence of placebo comparisons, severity of ADR, onset of ADR, formatting of ADRs in percentile (quantitative) format or qualitative format, whether references were used to cite information, and whether ADRs are grouped by organ system. Data was collected by two study investigators and discrepancies were resolved via consensus.Results:The seven DI mobile applications varied significantly on every analyzed ADR variable with the exception of ADR onset, which was absent in all databases. Significant differences were found for variables known to impact clinical judgment such as placebo comparisons and qualitative versus quantitative formatting. Placebo comparisons were most common among monographs in Lexicomp (30%) but were absent among monographs within other applications. Quantitative information was commonly used in most databases but was absent in Epocrates. Qualitative formatting was present in all Epocrates and Micromedex applications but absent in the majority of other applications. Substantial variations were also found in severity and grouping information.Conclusion:Substantial variation in ADR formatting exists among the most common DI mobile applications. These differences may translate into alternative interpretations of medical information and thus impact clinical judgment. Health care librarians and clinicians should consider ADR formatting when choosing between DI applications.     

Influence of upright versus time trial cycling position on determination of critical power and W′ in trained cyclists

Body position is known to alter power production and affect cycling performance. The aim of this study was to compare mechanical power output in two riding positions, and to calculate the effects on critical power (CP) and W′ estimates. Seven trained cyclists completed three peak power output efforts and three fixed-duration trial (3-, 5- and 12-min) riding with their hands on the brake lever hoods (BLH), or in a time trial position (TTP). A repeated-measures analysis of variance showed that mean power output during the 5-min trial was significantly different between BLH and TTP positions, resulting in a significantly lower estimate of CP, but not W′, for the TTP trial. In addition, TTP decreased the performance during each trial and increased the percentage difference between BLH and TTP with greater trial duration. There were no differences in pedal cadence or heart rate during the 3-min trial; however, TTP results for the 12-min trial showed a significant fall in pedal cadence and a significant rise in heart rate. The findings suggest that cycling position affects power output and influences consequent CP values. Therefore, cyclists and coaches should consider the cycling position used when calculating CP.     

Comfort assessment of running footwear: Does assessment type affect inter-session reliability?

Assessing comfort of running footwear reliably is challenging. The purpose of this study was to compare the intra-rater reliability between different assessment types, to calculate intra-individual reliability scores and to evaluate the effect of rater selection based on individual reliability scores on group level reliability. Three assessment types: ranking, Visual Analogue Scale (VAS), and Likert Scale (LS) were provided twice in six separate sessions among 30 participants, who assessed comfort of five shoes after treadmill running. Spearman's rho provided an evaluation of inter-session relative reliability and typical error as a measure of absolute reliability for each assessment type. Ranking (r?=?0.70, 95% confidence interval [CI] 0.61–0.78) yielded the highest relative reliability for overall comfort, followed by VAS (r?=?0.67, 95% CI 0.56–0.75) and LS (r?=?0.63, 95% CI 0.52–0.72), with large-scale overlaps of CIs between assessment types. The same order of assessment types was found for the percentage of reliable raters (r?≥?0.7) with 60% in ranking scale, 47% in VAS and 37% in LS. Forming subgroups corresponding to the intra-individual reliability substantially increased group level reliabilities. Based on measures of relative reliability, an extreme reduction in resolution as provided by the ranking from pairwise comparisons seems to be a valuable tool in footwear comfort assessments if assessment time is of minor importance. No preference can be provided for the two investigated rating scales. Besides the assessment type, a selection of the best raters in additional reliability checks seems to be a prerequisite for further comfort-related studies.     

物理核心素养评价与学业质量检测的研究

物理是初中阶段的重要课程,也是学生对世界进行基本科学认知的引导课程.在学生系统学习了近两年的物理后,学校和教师应对学生进行物理核心素养评价和学业质量检测.在物理核心素质评价与学业质量检测中,只有从物理观念、科学探究、科学思维、科学态度与责任入手,才能让评价与检测全面、丰富和多元.     

Temporal trends in 6-minute walking distance for older Japanese adults between 1998 and 2017

BackgroundThe 6-minute walking distance (6MWD) is an excellent measure of both functional endurance and health. The primary aim of this study was to estimate temporal trends in 6MWD for older Japanese adults between 1998 and 2017; the secondary aim was to estimate concurrent trends in body size (i.e., height and mass) and self-reported participation in exercise/sport.MethodsAdults aged 65–79 years were included. Annual nationally representative 6MWD data (n = 103,505) for the entire period were obtained from the Japanese Ministry of Education, Culture, Sports, Science and Technology. Temporal trends in means (and relative frequencies) were estimated at the gender–age level by best-fitting sample-weighted linear/polynomial regression models, with national trends estimated by a post-stratified population-weighting procedure. Temporal trends in distributional variability were estimated as the ratio of coefficients of variation.ResultsBetween 1998 and 2017 there was a steady, moderate improvement in mean 6MWD (absolute = 45 m (95% confidence interval (95%CI): 43–47); percent = 8.0% (95%CI: 7.6%–8.4%); effect size = 0.51 (95%CI: 0.48–0.54)). Gender- and age-related temporal differences in means were negligible. Variability in 6MWD declined substantially (ratio of coefficients of variation = 0.89, 95%CI: 0.87–0.92), with declines larger for women compared to men, and for 75–79-year-olds compared to 65–74-year-olds. Correspondingly, there were moderate and negligible increases in mean height and mass, respectively, and negligible increases in the percentage who participated in exercise/sport at least 3 days per week and at least 30 min per session.ConclusionThere has been a steady, moderate improvement in mean 6MWD for older Japanese adults since 1998, which is suggestive of corresponding improvements in both functional endurance and health. The substantial decline in variability indicates that the temporal improvement in mean 6MWD was not uniform across the distribution. Trends in 6MWD are probably influenced by corresponding trends in body size and/or participation in exercise/sport.