PCR技术在法医生物学中的应用

遗传学认为,DNA是人体性状及遗传变异的物质基础,它是遗传信息的携带者.DNA决定着我们的肤色、性别、身高、健康状况等个体特征.每个人的DNA都是不完全一样的.在法医学上,往往需要鉴别现场留下的物证是否属于犯罪嫌疑人.传统的方法主要是通过指纹、脚印、唇纹、化学物质等物证进行核对,这在一些情况下能起到作用.然而,有时候犯罪分子相当狡猾,或者现场被破坏的很严重,根本找不到上述痕迹.但是在理论上,只要犯罪分子在现场留下血渍、精斑、头发、阴毛、唾液等,通过分析其中的DNA,仍可查获真凶.另外,也可通过分析在现场找到的死者的残留物(如毛发、牙齿、骨骼等)中所含的DNA,来验明他们的真实身份.以前由于这些物证中DNA的含量甚微,再加上无法排除在分析时受到的干扰,DNA分析还或多或少是一种幻想.直到1985年,诺贝尔奖获得者Kary mullis发明了聚合酶链反应(polymerasechain reaction,PCR).再加上1988年Saiki对PCR的重大改进,才使DNA分析在法医学领域的应用取得了惊人的突破.所谓PCR,就是通过特定的DNA序列作为引物,在酶的作用下,经过几十次循环,使目的DNA得以快速复制、扩增,从而得到足以进行检测和分析的目的DNA片段.目的DNA(或称模板DNA)就好象是“轮子”,引物好象有汽车的“方向”,酶好象是“(?)力     

情感教学在语文教学中的运用

分析情感教学在语文教学中的重要意义,指出当前情感教育中存在的一些问题,并给出具体的解决办法.     

太极拳运动对大学生情绪紧张度的影响

情绪紧张度是人对客观现实的一种特殊反应形式,不同的情绪紧张度能够引起不同的机体反应。对练习太极拳及太极拳比赛的大学生的情绪紧张度进行了调查统计,并对生理、心理及行为障碍3个主要影响因素进行了分析论述。     

体育保险与构建2008年北京奥运风险转移模式研究

采用文献资料法,研究了体育保险与北京2008年奥运风险转移.分析发现:较为完善的险种,健全的体育保险法规和监督体制,以及有广泛的承保源是西方体育保险的发展特点;保险行业的介入为奥运风险的分散和转移提供了良好的途径.最后,对奥运风险存在的特点和奥运保险的国际通行做法进行了比较分析,并在此基础上提出应奥运风险构建北京2008年奥运风险转移模式.     

中国武术的“文化失语”与“文化认同”的思考——以体育全球化为背景

从中国武术“文化失语”这样一个现实环境展开研究,对中国武术失语现象作了概述与文化成因的解读、批判与反思。结论认为,“失语”现象值得警觉、反思,中国武术可以从中得到这样的关于文化认同建构的启示:中国武术需要发出自己的声音,需要有自己的一套话语系统;从而能与一切民族中优秀的文化进行对话,积极应对文化全球化;中国武术需要的是审视而不是误解。只有这样才能使武术更好地得到广泛的认同,并代表中国民族文化奏响其民族体育文化的强音。     

新东方主义：力比多实践中的认同政治——从《上海宝贝》到《我的禅》

通读卫慧的小说,其中所展现出的认同政治无疑耐人寻味：它到底是一种游移的身份更新（乃至脱胎换骨）,还是新瓶装旧酒？是国际化语境中游刃有余的本土展现、对抗,还是一种精明的利用？如果将卫慧置于全球化与跨国主义的语境中,考察其认同的变迁,我们不难发现她自我凝视的执著。不管是成名作《上海宝贝》,还是后起的《我的禅》都不能免俗。问题的更深一层在于,她在这种自我凝视中反映出认同的权力关系,或者认同媚俗。不管是价值取向,还是物质成本考量。     

体育教学中情感对学生积极性的影响

本文从心理学角度,阐述在体育教学中激发情感动力的作用,教师利用动作、语言、声调、问答等方式,充分调动学生的学习积极性,以取得良好的教学效果。     

A Social Development Curriculum: Applying Nurture Group Principles and Practices to Support Socially and Emotionally Vulnerable Children Within Mainstream Classrooms

Rebecca Doyle set up the first nurture group in Thetford Education Action Zone in 2000. In 2001, she published an account of her work to reintegrate pupils from the nurture group into the mainstream of their infant school in the pages of BJSE. In this article, Rebecca Doyle describes how mainstream class teachers requested further support in working with socially and emotionally vulnerable children in their classes using the principles and practices from the nurture group. A social development curriculum was written to support this interest, offering mainstream staff a planning tool to complement their existing schemes of work and to help in the drive to make the curriculum appropriate for every child, regardless of their barriers to learning. This article indicates that mainstream staff are now able to bring the increasing knowledge of nurture group working to the fore in their planning, supporting the development of whole-school nurturing approaches. The social development curriculum has become a well-used document alongside current planning frameworks, supporting staff in meeting the diverse needs of pupils within mainstream classrooms with minimal cost and little additional workload.
In closing her article, Rebecca Doyle argues that the social development curriculum has had a positive impact upon the pupils in her school and upon her colleagues on the staff. She is pursuing her research into nurturing approaches through her membership of the National Nurture Group Network and her studies for a higher degree at the University of East Anglia.     

对我国运动员科学选材及其管理的几点思考

科学选材一直以来是国家体育战略发展的关键环节，也是一个国家竞技体育事业兴衰的晴雨表。我国历来对运动员科学选材工作相当重视，也取得了一定成果。然而，近年来随着竞技运动激烈程度的不断提高，运动员淘汰率高，成材率低的现象屡见不鲜，本文针对这些问题，从理论和实践两个角度分析了出现这样矛盾的原因并提出了相关的建议。     

Conceptualizing employee identification with sport organizations: Sport Employee Identification (SEI)

The concepts of organizational identification and team identification have been researched heavily over the last half-century. However, scholars have failed to specifically examine organizational identification among sport employees. We develop a theoretical framework of organizational identification of sport employees, coined Sport Employee Identification (SEI). We conceptualize SEI as an amalgamation of organizational identification and team identification in which sport employees are both external (fans) and internal (employee) members of the sport organization. The development of the SEI model is based on related theory and further ethnographic data are collected over a four-month period within an intercollegiate athletics fundraising department. Implications for scholars and practitioners and avenues for future research are discussed.