Comparison of Mass Versus Activity of Creatine Kinase MB and Its Utility in the Early Diagnosis of Re-infarction

Currently employed markers for the detection of acute coronary syndrome are Troponin T, CK (Creatine Kinase) and CKMB activity. CKMB activity measured by immunoinhibition method can give falsely elevated results due to the presence of atypical CK and CKBB and at times lead to the mis-diagnosis of acute coronary syndrome. Hence, CKMB mass (CKMB) measured by electrochemiluminence sandwich principle was employed. In this cross-sectional study 183 samples of 61 patients were analyzed within 6 h of diagnosis of acute coronary syndrome and followed up to 72 h. The correlation coefficient between CKMB activity and CKMBM at 4–6 h was 0.744, while at 12–24 h it was 0.909 and at 48–72 h it was 0.337. Thus there was good association between the two methods at 12–24 h but, statistically for method comparison studies and for replacing one method by another, the two methods need to be in agreement with one another. In this study the two methods are not in agreement with one another and thus analytically not replaceable. Another finding was obtained that CKMBM reached cut off levels prior to CKMB enzyme activity and hence, CKMBM is clinically better than CKMB activity to detect reinfarction.     

Changes in running endurance performance following intermittent altitude exposure simulated with tents

Abstract

The effect of intermittent hypoxia on sea-level endurance performance was assessed by using hypoxic tents to simulate the live high-train low approach to altitude training. Eleven male sub-elite competitive runners and triathletes participated in a crossover study of usual training (control) and usual training with altitude exposure (altitude). Altitude treatment consisted of 25±3?d (mean±SD) of sleeping in tents for 8.1±0.6?h.d^?1, progressing from a simulated altitude of 2500?m to 3500?m above sea level. Washout period between control and altitude treatments was 4?wk. Three treadmill runs to exhaustion lasting ～2, ～4 and ～8?min were completed 7 and 12?d after control and altitude treatments. Times for standard competition distances (800, 1500 and 3000?m), were predicted using a log-log model, improved by 1.0% (90% confidence limits, ±1.3%), 1.4% (±1.2%) and 1.9% (±1.5%), respectively. Improvements were greater in the six athletes with an I allele for angiotensin converting enzyme (ACE): 2.3% (±1.5%), 2.2% (±1.5%), and 2.1%, (±2.1%), respectively. Effects of simulated altitude on hemoglobin concentration were unclear. Altitude exposure simulated with hypoxic tents is likely to enhance performance substantially in middle-distance endurance running events, especially for individuals with an I allele of the ACE gene.     

三唑酮对蚕豆离体叶片中三种抗氧化酶活性的影响

以蚕豆叶片为试材,对其进行黑暗诱导衰老,研究了三唑酮处理对蚕豆离体叶片过氧化物酶（POD）、超氧化歧化酶（SOD）、过氧化氢酶（CAT）活性的影响．结果显示,在蚕豆离体叶片衰老过程中,POD活性不断升高,特别是在48h之前十分明显,SOD,CAT活性在48h之前上升,然后有所下降;经三唑酮处理蚕豆离体叶片,三种抗氧化酶活性在处理的前期上升速率明显加快,而且维持上升的时间更长．在整过处理过程中三种抗氧化酶活性均远高于对照组．表明三唑酮处理蚕豆离体叶片后,可以通过提高并维持三种抗氧化酶的活性,增强蚕豆叶片抗氧化能力,进而延缓其衰老进程．     

酶解法制取蚕蛹多肽

以蚕蛹为原料,经脱脂后,采用碱法提取蚕蛹蛋白,以双酶水解的方法制取可溶性蚕蛹多肽,最佳制取条件为固液比1:10,水解温度45℃,酶用量3%(木瓜蛋白酶:胰蛋白酶=1:1),水解时间6h,在此条件下制备的蚕蛹多肽得率为62.5%。使用胃蛋白酶和胰蛋白酶分别水解时,多肽得率为58.7%。     

Protection of salvianolic acid B for human endothelial cells against hydrogen peroxide-induced oxidative damage

Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured: Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage ' was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge.     

花粉运动休闲饮料的研制

以蛋白质提取率为主要指标,对花粉的发酵破壁提取和酶法破壁提取进行了研究．实验表明,当花粉加2．5倍水过胶体磨细化后,采用酶法提取工艺,在温度为45℃,pH为4．6的条件下,酶解30h,然后在60℃下浸泡10h,蛋白质提取率最高,效果最好．通过对花粉运动休闲饮料的调配试验,当花粉母液为20％、氯化钠为2％、蛋白糖为0．04％、柠檬酸为0．1％时,饮料的品质最佳．     

男子赛艇运动员冬训期间血清酶活性及心电图的变化

结合武汉体育学院男子赛艇实际冬训过程,比较优秀运动员与普通运动员不同强度训练周后血清酶CK、CK-MB、LDH、LDH1水平与心电图∑T/R值的变化,探讨系统赛艇训练对运动员机能能力及心脏功能的影响.     

穴位和非穴位刺激对大鼠骨骼肌能量代谢的影响

采用实验法,将大鼠随机分成非穴位刺激组、穴位刺激组(刺激"足三里"、"承山"、"大椎"、"命门"穴位)进行电针刺激,观察大鼠骨骼肌ATP酶、乳酸脱氢酶(LDH)和磷酸肌酸激酶(CPK)活性的变化。结果表明:穴位电刺激使大鼠骨骼肌ATPase、LDH和CPK活性比非穴位刺激组显著升高(P<0 01),并对协调发展磷酸肌酸(CP)供能,糖酵解供能能力有明显效果,说明了穴位电刺激能改善大鼠骨骼肌的能量代谢。     

碱性蛋白酶高产菌株的筛选

实验采用土壤为样品,对样品进行分离,纯化,培养,筛选出了3株产酶能力较高的菌株,即R1、R2、R3。复筛确定了R1的产酶能力相对较高。通过相对酶活力的测定,确定了反应最佳温度为30℃,最佳pH为9。菌株在最适条件下产酶能力达246.8U/ml。     

急慢性乙醇中毒对大鼠心脏的影响

目的：探讨急慢性乙醇中毒对大鼠心脏的影响机制．方法：雄性健康SD大鼠,随机分为急性和慢性对照组、急性和慢性乙醇中毒组,每组8只．酶法和比色法检测血清和心肌组织谷草转氨酶（AST）、肌酸磷酸激酶（CPK）、超氧化物歧化酶（SOD）、丙二醛（MDA）、Ca^2＋-Mg^2＋-ATPase和Na^2＋-K^＋-ATPase的含量,观察心肌微细结构的改变．结果：与对照组相比：急、慢性乙醇中毒＋组心肌MDA、AST、CPK和血清AST、CPK含量升高（P〈0．05,P〈0．01）,SOD含量减少（P〈0．05,P〈0．01）,Na^2＋-K^＋-ATPase和Ca^2＋-Mg^＋-ATPase活性降低（P〈0．05,P〈0．01）．心肌组织HE染色显示,乙醇中毒组大鼠心肌细胞肿胀、细胞界限模糊不清,慢性组尤为明显．结论：急慢性乙醇中毒通过对大鼠心肌酶和氧自由基的影响而对心脏产生抑制作用．