清酒发酵技术研究

采用大米为原料 ,以纯种培养的酵母为发酵剂 ,用小曲为糖化剂 ,在常温下发酵 8～ 9天酿制清酒。成品颜色淡黄 ,酒度高 ,酒质淡丽爽口 ,经蔗糖和蛋白糖调配后 ,甜醇可口 ,风味较好。此外 ,还以复合酶制剂为糖化剂试酿了全酶法清酒。成品酒的主要指标和小曲清酒接近     

腐植酸钾浸种对玉米幼苗保护酶及MDA的影响

探索腐植酸钾浸种对提高作物生长的抗盐性及其在作物生长化学调控中的作用。采用不同浓度（0、300、400、500／xg／mL）的腐植酸钾浸种玉米,研究了在100mmol／LNaCl的盐胁迫环境下,三叶期玉米幼苗叶保护酶活性及丙二醛含量的变化。与对照相比,浸种玉米的超氧化物歧化酶活性增高,但无显著差异;浸种玉米的过氧化物酶活性增高,在4013·雌／mL时达极显著水平,在500μg／mL时达显著水平;浸种玉米的过氧化氢酶活性增高,在300、400μg/mL时分别达显著水平,在500μg／mL时达极显著水平;浸种玉米的丙二醛含量降低,在300—500μg／mL范围内达显著水平。腐植酸钾浸种玉米,可减轻玉米在盐胁迫下的膜脂过氧化程度,提高玉米抗盐性。     

中医药保肝降酶治疗慢性乙型病毒性肝炎进展

慢性乙型肝炎患者,因HBV在体内不断复制,肝脏出现炎症坏死及其肝纤维化,导致病情进展、恶化,因此,对于ALT明显升高者或肝组织学明显炎症坏死者,在抗病毒治疗的基础上,当选用抗炎保肝药物尤其重要。中医药在慢性肝炎的保肝降酶治疗方面,积累了丰富的经验,提供了坚实的临床基础,文章从中医防治慢性乙型肝炎的理论基础、慢性乙型肝炎的辨证分型与肝功能的关系、中药复方及中药单体成分对慢性乙型肝炎的保肝降酶作用等方面,将近年来中医药对慢性乙型肝炎的保肝降酶的研究进行了综述。     

酶工程课程改革、实验室建设的思考与探索

酶工程是高等院校生物技术、生物工程专业的核心课程.结合教改实践,阐述了酶工程课程体系、教材建设、课堂教学、实验室建设等的教改实践,并对该课程建设中存在的问题和困境,提出了解决的设想.     

镍胁迫对大豆种子萌发过程中的生理生化影响

以两个大豆品种（周豆11号和豫豆24号）为材料，研究了不同浓度Ni^2＋处理对大豆种子萌发和幼苗生长中膜脂过氧化水平、细胞膜透性及保护酶（SOD、POD、CAT）活性的影响．结果表明，（1）Ni^2＋浸种处理对大豆种子萌发具有低浓度下的激活效应和高浓度下的抑制效应；（2）细胞内丙二醛（MDA）含量和细胞膜透性在低浓度Ni^2＋处理下略有降低，尔后随着镍浓度的提高其质膜透性和MDA含量逐渐增大，体内保护酶系统中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性的变化表现为先升后降的趋势．不同大豆品种对NP的反应存在霉鼻与抗氢化系统的整体活力有关．     

真菌的分子生物学鉴定方法

真菌的分子生物学鉴定的方法及原理,真菌DNA碱基组成的分类鉴定、DNA分子杂交、真菌mtDNA限制性片段长度分态性的分析以及随即扩增多态性DNA分析。     

Utilization of an Accelerated Queso Fresco Recipe to Teach Concepts of Food Science in a Didactic Program in Dietetics

Students in the Didactic Program in Dietetics (DPD) at the University of Maine were exposed to the cheese‐making process, within a lab setting of two hours, utilizing an accelerated recipe for a Queso Fresco‐style cheese. The purpose of this project was to provide students with a novel, hands‐on learning experience, which covered concepts of coagulating milk with enzymes and the treatment of curds during the cheese‐making process. The cheese recipe was tested three times by faculty and a teaching assistant to ensure standard results. The recipe, directions and procedures were adjusted and recorded. A standardized recipe and procedure were created for use in the student laboratory. Comparison of knowledge pre‐ and post‐experiment were analyzed using the Wilcoxon Signed‐Rank Test using Statistical Package for Social Sciences (SPSS) version 23.0. Thirty‐four students participated in the laboratory and when surveyed, expressed significant improvement in their understanding of the cheese‐making process (p <0.001). Students also rated themselves on a six‐step confidence scale from “not confident at all” to “very confident” in their understanding and ability in cheese‐making with 70.5 % of the 34 students participating reporting a three‐step increase in their confidence level. Students also completed a sensory evaluation of the recipe, utilizing a 9‐point Hedonic scale (1‐Dislike Extremely to 9‐Like Extremely), with over 88 % of students giving the product a positive overall acceptability rating. Through experience gained during the cheese‐making laboratory, students were able to apply concepts in food preparation, food science and sensory evaluation, all part of the curriculum content requirements of a DPD.     

潘南金治疗心律失常的临床观察

本文应用潘南金（PANAGIN）治疗缺血性心脏所致心律失常共36例，观察结果患者症状缓解明显，心律失常治疗有效率达85.6％，副作用少，安全性大，可作为抗心律失常治疗的辅助药物，值得在临床上应用和作一步的研究．     

金属离子对黄粉虫NAGase活力的影响

以黄粉虫为材料,以N-乙酰--βD-氨基葡萄糖苷酶(NAGase,EC 3.2.1.52)为研究对象.研究各种金属离子对NAGase酶活力的影响.结果表明,正一价碱金属Li 、Na 、K 等对酶活力基本上没有效应;Ca2 、Mg2 、Ba2 等碱土金属离子对酶活力也没有显著影响;而正三价金属离子Al3 和Fe3 、过渡金属离子Zn2 和Cd2 、重金属离子Cu2 、Ag 、Hg2 和Pb2 等对该酶活力均有不同程度的抑制作用.其中,Hg2 的抑制作用最为显著,2.5 mmol/L Hg2 可使酶活力完全丧失.进一步研究了Cu2 的抑制作用动力学,结果显示:Cu2 对酶的抑制作用为非竞争性可逆抑制,抑制常数KI=KIS=5.5 mmol/L.     

Science Letters：Alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs

An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotobacter. However, the vital seedling parameters such as germination percentage and vigour index were improved. Azotobacter treatment influenced maximum of 50% germination, whereas Azospirillum and Azotobacter were on par with C. roseus with respect to their vigour index. There was significant difference in the population of total diazotrophs. Azospirillum and Azotobacter between rhizosphere and non-rhizosphere soils of C. roseus had the same trend and were observed at various locations of the study. The activities of antioxidant enzymes such as superoxide dismutase （SOD）, peroxidase （POX） and catalase （CAT） were increased to a significant extent due to the treatment with diazotrophs.