Cu^2＋敏感水溶性萘酰亚胺高分子荧光探针的合成及其光学性能

以1,8-萘二甲酸酐、二乙烯三胺（DETA）、N-甲基哌嗪及丙烯基氯为原料,通过酰胺化、季胺化、SN2亲核取代以及丙烯酰胺共聚合等反应,合成了水溶性1,8-萘酰亚胺高分子荧光探针。用紫外光谱、荧光光谱等手段研究它们在水、四氢呋喃和乙醇溶液中光物理化学性质,同时考察浓度和溶剂极性及取代基对荧光性能影响及对金属离子的识别作用。结果表明,此高分子荧光探针的光稳定性及荧光量子产率明显提高,随着溶剂极性增大,荧光量子产率增大,波长红移;当浓度超过8×10^-4g/m L时出现荧光浓度自猝灭;该探针在水中能对Cu^2＋在392 nm处进行高选择性识别。     

大理市售生活纸制用品中荧光增白剂污染状况检测分析

目的：了解大理市市售纸制用品中荧光增白剂污染的状况,为保护人民的身体健康和纸质用品监督管理提供一定的参考依据。方法：采用荧光分光光度法对荧光增白剂进行测定,用SPSS16.0进行统计分析。结果：不同品质的卷筒纸荧光增白剂的含量不同,各纸制品中荧光增白剂检测结果比较,差异具有统计学意义。纸饭盒经浸泡处理后荧光增白剂的溶出率为82.38%。结论：大理市售的生活纸制用品均检出荧光增白剂,为减少荧光增白剂对居民健康的影响,应加强对生活纸质用品的监督管理。     

荧光粉生产废水的加载-还原-协同絮凝工艺处理

彩显生产过程中,荧光粉废水水质水量变化幅度大,运行成本高,污染物排放量高．采用加载一还原一协同絮凝工艺处理荧光粉废水,工程实践表明,在处理效率、出水水质及运行成本、产泥率等方面都远优于传统工艺．该工艺出水的SS,CODcr,TCr,Cr＋6,产泥率及运行成本的平均值分别为0.7mg／L,16．1mg／t,9．7mg／L,1．3kg／m3,1．26元／m3,较原有工艺降低超过35％,其出水水质优于《城市污水再生利用城市杂用水水质》GB／T18921-2002标准,可回用作杂用水及景观水补水．     

A system for screening agonists targeting β2-adrenoceptor from chinese medicinal herbs

In order to develop a model for screening the agonists of human β₂-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor (GPCR) regulation mechanism and destabilized enhanced green fluorescent protein (d₂EGFP) reporter gene technique. The positive cell clone was confirmed by real-time polymerase chain reaction (PCR) and imaging analysis. To assess the value of this model, we screened over 2000 high performance liquid chromatography (HPLC)-fractionated samples from the ethanol extracts of Chinese medicinal herbs. Six fractions (isolated from Panax japonicus, Veratrum nigrum, Phellodendron amurense, Fructus Aurantii Immaturus, Chaenomeles speciosa, and Dictamnus dasycarpus) showed significant effects on active reporter gene expression, three of which (isolated from Phellodendron amurense, Fructus Aurantii Immaturus, and Chaenomeles speciosa) were selected for further concentration response analysis and the half maximal effective concentration (EC_{1/2 max}) values were 4.2, 2.7, and 4.8 μg/ml, respectively. Therefore, this reporter gene assay was suitable for screening β₂-adrenoceptor agonists. The results suggest that the six herbal extracts are the possible agonists of β₂-adrenoceptor. Project (No. 30873103) supported by the National Natural Science Foundation of China     

紫外照相技术在检测假单胞菌荧光铁载体中的应用

铁载体的检测方法有通用的络天氰(CAS)检测法,但未见针对荧光类铁载体的检测方法相关报道.本研究采用紫外照相技术实现了对荧光铁载体进行原位检测,结果显示此方法对荧光铁载体获得较好的效果.     

EGFP基因转染骨髓间充质干细胞的实验研究

目的:探讨外源性EGFP基因转染修饰兔骨髓间充质干细胞(m esenchymal stem cells,MSCs)的可行性。方法:用增强型荧光绿色蛋白(enhanced green fluorescent protein,EGFP)作为报告基因,以脂质体法转染骨髓间充质干细胞,观察转染结果、表达情况及对靶细胞活力的影响。结果:经荧光显微镜下观察证实:成功地对骨髓间充质干细胞实现了基因转染。结论:兔骨髓间充质干细胞能够在体外适宜的条件下进行长期培养;骨髓间充质干细胞可直接作为基因靶细胞,建立稳定表达EGFP的骨髓间充质干细胞系具有可行性,为进一步做标记的MSCs细胞的移植研究奠定基础。     

Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass

Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with Agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4-D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.     

细胞内DNA和RNA显色反应的改进

利用经典的细胞生物学染色方法显示细胞内DNA与RNA,在实际教学过程中出现与理论上有较大的偏差,经对染色方法进行改进并进行了多次比较实验,获得了较为理想的结果,该实验结果将为医学细胞生物学实验课教学提供一个良好的方法。加深学生对细胞内DNA与RNA分布的理解。     

基于磺酸类席夫碱锌、镉配合物的TG-DTG和荧光性质研究

合成了两种席夫碱配合物[Zn(OBSP)2].2H2O(I)和[Cd(OBSP)2].2H2O(II)(KOBSP邻氨基苯磺酸缩吡啶—2—甲醛席夫碱的钾盐),并进行了热重和红外分析,最后研究了它们的荧光性质。结果表明,配合物(I)(II)的荧光来源可能是配体内部的π→π*的电荷跃迁引起的。     

具有毒性质粒沙门菌荧光PCR快速检测试剂盒的研发

介绍了用于检测具有毒性质粒沙门菌的2种荧光PCR试剂盒（一种是荧光PCR染料法检测试剂盒,另一种是荧光PCR探针法检测试剂盒）的研制过程,2种试剂盒运用了自行设计的特异性引物和TaqMan探针,能同时特异性地从众多的沙门氏菌和非沙门氏菌中高效、快速、准确地检测出多种具有毒性质粒沙门氏菌,而对于不具有毒性质粒的沙门氏菌和非沙门氏菌均不能检出,可为食品检验和医疗诊断领域提供方便快捷检测具有毒素质粒沙门氏菌的荧光PCR试剂盒．