荧光探针在植物激素检测中的应用进展

本文综述了荧光探针在植物激素检测方面的一些应用现状,如荧光探针用于检测茉莉酸、脱落酸、油菜素类固醇和多胺等。     

Use of antigen prepared from in vitro culture ofPlasmodium falciparum for the detection of antibodies in sera from patients exposed toPlasmodium vivax

The blood stage parasites fromin vitro cultures ofPlasmodium falciparum were employed for the detection of malaria antibodies in patient sera havingP. vivax infections employing indirect fluorescent antibody (IFA) test. The antibody titres obtained for theP. vivax infected sera ranged from 1∶128 to 1∶1024. Control slides smeared with non-immune sera did not show any fluorescence. On the basis of the results of the present study it maybe concluded that antigen prepared fromin vitro cultures ofP. falciparum cross-reacts withP. vivax antibodies.     

绿色荧光蛋白及其在分子生物学上的应用

许多海洋无脊椎动物体内都含有绿色荧光蛋白,这种蛋白质结构很特殊,在受到蓝光或紫外线刺激时可以发射绿色荧光,并不需要任何协同因子、底物,适合用作普遍的报告标记,尤其适合于活体细胞或组织。而且它发出的荧光稳定,检测简单,结果真实可靠。并且GFP对光漂白、氧化剂、还原荆以及其他许多化学试剂具有极强的稳定性．易于构建载体。它独特的性质引起了生物学界的广泛关注。本文简要地概述了绿色荧光蛋白及其在分子生物学上的应用,包括蛋白融合,细胞筛选,定位标记,基因表达调控,计算细胞生长速度等。     

Applications of smartphone-based near-infrared (NIR) imaging,measurement, and spectroscopy technologies to point-of-care (POC) diagnostics

The role of point-of-care(POC)diagnostics is important in public health.With the support of smartphones,POC diagnostic technologies can be greatly improved.This opportunity has arisen from not only the large number and fast spread of cell-phones across the world but also their improved imaging/diagnostic functions.As a tool,the smartphone is regarded as part of a compact,portable,and low-cost system for real-time POC,even in areas with few resources.By combining near-infrared(NIR)imaging,measurement,and spectroscopy techniques,pathogens can be detected with high sensitivity.The whole process is rapid,accurate,and low-cost,and will set the future trend for POC diagnostics.In this review,the development of smartphone-based NIR fluorescent imaging technology was described,and the quality and potential of POC applications were discussed.     

细胞器超分辨有机荧光探针技术专利分析

设计和开发靶向细胞器的荧光探针,对于细胞器结构相关的细胞生理和病理过程的探究具有重要意义.本文主要对细胞器有机荧光探针领域进行专利检索与分析,目的在于整理和分析该领域复杂的专利技术情报,用图表的形式将重要情报展现出来,以了解该领域的专利现状,为该领域科研人员提供情报支持.     

全植床深板层角膜移植术后神经重建实验研究

目的观察新西兰兔全植床深板层角膜移植术后不同时期的角膜神经重建过程。方法新西兰兔麻醉后行冰冻保存角膜全植床深板层角膜移植术。术后分别于第7、21天,第1、2、3、4、6个月摘取术眼角膜,行乙酰胆碱酯酶神经染色,光学显微镜下观察。结果术后7天,植片内神经溃变,同时植床远端未损伤神经发出分支,穿过创缘进入植片。术后21天,神经溃变完成。术后1个月,植片内较多细、长、直的神经纤维,创缘外侧植床基质神经发出较多分支平行于创缘,但未进入植片。术后3个月,植床近端基质神经同一部位同时发出2￣4个分支进入植片,植片内细、长、直的神经纤维达到最多。术后4￣6个月,植片内神经重新塑型。少数植床近端基质神经形态基本接近正常。结论全植床深板层角膜移植术后神经再生恢复较慢。     

A system for screening agonists targeting β2-adrenoceptor from Chinese medicinal herbs

In order to develop a model for screening the agonists of human 132-adrenoceptor from Chinese medicinal herbs extracts, we used a cell-based functional assay based on a common G protein-coupled receptor (GPCR) regulation mechanism and destabilized enhanced green fluorescent protein (d2EGFP) reporter gene technique. The positive cell clone was confirmed by real-time polymerase chain reaction (PCR) and imaging analysis. To assess the value of this model, we screened over 2000 high performance liquid chromatography (HPLC)-fractionated samples from the ethanol extracts of Chinese medicinal herbs. Six fractions (isolated from Panax japonicus, Veratrum nigrum, Phellodendron amurense, Fructus Aurantii Imrnaturus, Chaenomeles speciosa, and Dictamnus dasycarpus) showed significant effects on active reporter gene expression, three of which (isolated from Phellodendron amurense, Fructus Aurantii lmmaturus, and Chaenomeles speciosa) were selected for further concentration re-sponse analysis and the half maximal effective concentration (ECI/2 max) values were 4.2, 2.7, and 4.8 μg/ml, respectively.Therefore, this reporter gene assay was suitable for screening β2-adrenoceptor agonists. The results suggest that the six herbal extracts are the possible agonists of β2-adrenoceptor.     

双三嗪-肉桂酸聚合型荧光增白剂的光物理化学性质研究

以肉桂酸盐替代其中一种氨基化合物,经过三步缩合反应和苯乙烯的共聚反应,合成了系列双三嗪-肉桂酸聚合型荧光增白剂（PFBs）。用紫外光谱、荧光光谱、光致异构化及应用试验等手段研究它们在水和N,N’-二甲基甲酰胺（DMF）溶液中光物理化学性质及增白性能,同时考察浓度和溶剂极性及化合物结构对荧光性能影响。结果表明此双三嗪-肉桂酸聚合型荧光增白剂,可明显提高光的稳定性及荧光量子产率,降低光致异构现象;同时随着溶剂极性增大,荧光量子产率增大,荧光波长红移;当浓度超过1×10^-4g/mL时出现荧光浓度自猝灭。同时三嗪环上取代基类型直接影响顺反式异构体的比例,但对光吸收和荧光分配并无显著影响。     

荧光增白剂在无磷洗衣粉中效能讨论

以常用表面活性剂LAS、AEO—9和助剂结晶型层状硅酸钠、4A沸石、荧光增白剂VBL、31配成实验用无磷洗衣粉。通过粉体白度实验，去污实验和循环洗涤实验，考察了荣光增白剂在洗衣粉中的效能．实验结果表明：添加荧光增白剂使物体白度提高；去污力比值提高，荧光增白剂VBL、31在农洗衣粉中的最佳添加量其质量分数是0.15%。     

刍论计算机在分子荧光光谱分析中的联机数据处理

重点阐述了计算机在分子荧光光谱分析过程中的联机数据处理功能，对充分掌握和利用计算机去进行高效优质且准确灵便的分子荧光光谱分析联机数据处理有知物善用之益。