DOMS和离心收缩导致的肌肉损伤的关系

不习惯的离心运动均可导致延迟性肌肉酸痛（DOMS）。离心运动导致的损伤和炎症反应诱发了DOMS。然而，近来的研究不支持此假说，DOMS起因的观点受到质疑。就近来关于DOMS和肌肉损伤关系的相关文章作一综述并就其争议点提出见解。     

乳腺癌的免疫调节治疗(英文)简

Standard treatment options for breast cancer include surgery, chemotherapy, radiation, and targeted therapies, such as adjuvant hormonal therapy and monoclonal antibodies. Recently, the recognition that chronic inflammation in the tumor microenvironment promotes tumor growth and survival during different stages of breast cancer development has led to the development of novel immunotherapies. Several immunotherapeutic strategies have been studied both preclinically and clinically and already have been shown to enhance the efficacy of conven- tional treatment modalities. Therefore, therapies targeting the immune system may represent a promising next-generation approach for the treatment of breast cancers. This review will discuss recent findings that elucidate the roles of suppressive immune cells and proinflammatory cytokines and chemokines in the tumor-promoting microen- vironment, and the most current immunotherapeutic strategies in breast cancer.     

Effects of AM3 (Inmunoferon®) on increased serum concentrations of interleukin-6 and tumour necrosis factor receptors I and II in cyclists

Abstract

The aims of this study were to examine the changes in plasma concentrations of inflammatory cytokines induced by training and competition in professional cyclists. We report the serum concentrations of interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-α), tumour necrosis factor receptors I and II (TNFR-I and -II) in a prospective, randomized, double-blind trial involving the administration of AM3 (Inmunoferon®), an oral booster immunomodulator, or placebo to 16 professional cyclists (n=8 in each group) for 65 consecutive days. Serum was collected just before treatment began (baseline), at the end of pre-competition training, before the mountain stage of the competition (60 days), 4 h after finishing this stage (62 days), and 18 h after the fifth and last day of competition (65 days). To determine the normal levels of cytokines and soluble TNF receptors, individual samples from 14 moderately trained healthy controls were studied. After 60 days of training, the serum concentrations of IL-6 did not differ significantly from those at the beginning of the study for either group of cyclists (placebo and AM3). A significant rise was seen in IL-6 concentrations in both the AM3 and placebo groups at 62 days, 4 h after finishing the mountain stage. The increase was significantly greater in the placebo group than in the AM3 group. At 65 days of treatment, 18 h after the fifth and last day of competition, IL-6 concentrations were similar to those recorded at the end of the training, but were significantly higher in the placebo group than in the AM3 group. At the end of training, serum TNFR-I concentrations in both groups of cyclists were significantly lower than at baseline. The concentrations of serum TNFR-I and -II both 4 h after finishing the mountain stage and 18 h after the fifth and last day of competition were significantly higher than those recorded after training in both groups. Professional cycling competition is associated with increases in serum IL-6 and TNFR-I and -II concentrations. Inmunoferon treatment reduced significantly the concentrations of IL-6 but not those of TNFR-I and -II.     

林丹暴露诱导肝脏HepG2细胞的毒性效应研究

有机氯农药林丹具有高毒性、亲脂性、化学稳定性和生物富集性,曾被广泛应用于农业生产中。林丹严重威胁着生态环境和生物安全,体内富集后造成肝功能损伤,但其对肝脏的毒性效应尚不十分清楚。在本研究中,肝脏HepG2细胞经不同浓度林丹暴露处理24 h后,使用噻唑蓝（MTT）、2,7-二氯荧光素（DCF）和免疫荧光方法分别检测细胞活力、细胞内活性氧（ROS）和自噬生成;并用实时定量PCR和Western blot分别检测细胞IL-6、IL-8、IL-1β、TNF-α、NF-κB、Beclin1、ULK1 mRNA和P-p65、IL-1β、P62、LC3蛋白质的表达水平。结果表明,高浓度林丹暴露显著抑制HepG2细胞活力,升高细胞内的ROS和乳酸脱氢酶（LDH）生成水平,降低过氧化氢酶（CAT）活性,并明显上调TNF-α、IL-6、IL-1β、IL-8、NF-κB mRNA和P-p65、IL-1β、P62、LC3、NF-κB蛋白质表达水平。以上结果表明,林丹诱导肝脏HepG2细胞的氧化应激和自噬,并激活NF-κB信号通路引起细胞的炎症反应。     

Relationship between serum interleukin-1beta levels and acute phase response proteins in patients with familial Mediterranean fever

Introduction:

The aim of this study was to investigate whether serum levels of interleukin-1β (IL–1β) has any possible correlation on inflammatory parameters such as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and fibrinogen concentration in patients with familial Mediterranean fever (FMF) patients during attack-free period.

Materials and methods:

The serum levels of IL-1β, as an indicator of cytokines status, and the acute phase response proteins, CRP, ESR and fibrinogen levels were evaluated in 35 attack-free patients with FMF and 25 healthy volunteers.

Results:

Serum IL-1β levels were significantly higher in patients with FMF than control subjects (P = 0.018). There was no statistically significant difference in the serum levels of ESR, CRP and fibrinogen between two groups (P = 0.181, P = 0.816, P = 0.686, respectively). There was a significant correlation between IL-1β and CRP (r = 0.513, P = 0.002) values of FMF group.

Conclusions:

In conclusion, our results confirm the presence of increased IL-1β levels in FMF patients during attack-free period. Serum IL-1β values seems to correlate with CRP levels. The elevation of IL-1β levels may be important in monitoring subclinical inflammation of attack free period in FMF patients.     

Reallocating sitting time to standing or stepping through isotemporal analysis: associations with markers of chronic low-grade inflammation

Although high levels of sitting time are adversely related to health, it is unclear whether moving from sitting to standing provides a sufficient stimulus to elicit benefits upon markers of chronic low-grade inflammation in a population at high risk of type 2 diabetes (T2DM). Three hundred and seventy two participants (age = 66.8 ± 7.5years; body mass index (BMI) = 31.7 ± 5.5kg/m²; Male = 61%) were included. Sitting, standing and stepping was determined using the activPAL3^TM device. Linear regression modelling employing an isotemporal substitution approach was used to quantify the association of theoretically substituting 60 minutes of sitting per day for standing or stepping on interleukin-6 (IL-6), C-reactive protein (CRP) and leptin. Reallocating 60 minutes of sitting time per day for standing was associated with a ?4% (95% CI ?7%, ?1%) reduction in IL-6 (p = 0.048). Reallocating 60 minutes of sitting time for light stepping was also associated with lower IL-6 levels (?28% (?46%, ?4%; p = 0.025)). Substituting sitting for moderate-to-vigorous (MVPA) stepping was associated with lower CRP (?41% (?75%, ?8%; p = 0.032)), leptin (?24% (?34%, ?12%; p ≤ 0.001)) and IL-6 (?16% (?28%, 10%; p = 0.036). Theoretically replacing 60 minutes of sitting per day with an equal amount of either standing or stepping yields beneficial associations upon markers of chronic-low grade inflammation.     

Escherichia coli lipopolysaccharide administration alters antioxidant profile during hypercholesterolemia

Pathogens, especially Gram-negative bacteria or bacterial endotoxin, along with other classical factors, may be involved in inflammatory response within the aortic endothelium during the progression of cardiovascular disease. Studies have shown that bacterial endotoxin activates various inflammatory processes in the body. Our study aims to establish a correlation between endotoxemia and vascular expression of antioxidant enzymes. Swiss albino mice (4 weeks old) were fed a high fat diet for 24 weeks and then were administered Escherichia coli endotoxin intraperitonealy, for 4 weeks. Tissue antioxidant enzymes, serum levels of IL-6 and TNF alpha were measured from the mice. We report that i.p. administration of endotoxin to hyperlipidemic mice resulted in elevation of superoxide dismutase and catalase enzymes, which was paralleled by a systemic reduction of serum cholesterol and LDL expression. Myeloperoxidase levels were also found to be elevated in aortic tissue, while an increase was also observed in the serum cytokine levels.     

Differential time responses in inflammatory and oxidative stress markers after a marathon: An observational study

ABSTRACT

Acute and adaptive changes in systemic markers of oxidatively generated nucleic acid modifications (i.e., 8-oxo-7,8-dihydro-2?-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo)) as well as inflammatory cytokines (i.e., C-reactive protein, interleukin-6, interleukin-10, and tumour necrosis factor alpha), a liver hormone (i.e., fibroblast growth factor 21 (FGF21)), and bone metabolism markers (sclerostin, osteocalcin, C-terminal telopeptide, and N-terminal propeptide of type 1 procollagen) were investigated following a marathon in 20 study participants. Immediate changes were observed in inflammatory cytokines, FGF21, and bone metabolism markers following the marathon. In contrast, no immediate changes in urinary excretion of 8-oxodG and 8-oxoGuo were evident. Four days after the marathon, decreased urinary excretion of 8-oxodG (-2.9 (95% CI -4.8;-1.1) nmol/24 h, P < 0.01) and 8-oxoGuo (-5.8 (95% CI -10.3;-1.3) nmol/24 h, P = 0.02) was observed. The excretion rate of 8-oxodG remained decreased 7 days after the marathon compared to baseline (-2.3 (95%CI -4.3;-0.4) nmol/24 h, P = 0.02), whereas the excretion rate of 8-oxoGuo was normalized. In conclusion marathon participation immediately induced a considerable inflammatory response, but did not increase excretion rates of oxidatively generated nucleic acid modifications. In fact, a delayed decrease in oxidatively generated nucleic acid modifications was observed suggesting adaptive antioxidative effects following exercise.     

Nutritional needs of elite endurance athletes. Part I: Carbohydrate and fluid requirements

Abstract

Both carbohydrate depletion and dehydration have been shown to decrease performance whilst severe dehydration can also cause adverse health effects. Therefore carbohydrate and fluid requirements are increased with exercise. Ingestion of 200–300?g of CHO 3–4?h prior to exercise is an effective strategy in order to meet daily CHO demands and increase CHO availability during the subsequent exercise period. There is little evidence that CHO during the hour immediately prior to exercise has adverse effects such as rebound hypoglycaemia. CHO ingestion during exercise has been shown to improve performance as measured by enhanced work output or decreased exercise time to complete a fixed amount of work. Recent studies have demonstrated that exogenous CHO oxidation rates can be increased by ingesting combinations of CHO that use different intestinal CHO transporters. After exercise maximal muscle glycogen re-synthesis rates can be achieved by ingesting CHO at a rate of ～1.2?g/kg/h, in relatively frequent (e.g., 15–30?min) intervals for up to 5?h following exercise. Protein amino acid mixtures may increase glycogen synthesis further but only if relatively small amounts of CHO are ingested.

Hypohydration and hyperthermia alone have negative effects on performance but their combination is particularly serious, both in terms of performance and health. Dehydration can be prevented by fluid ingestion pre exercise and during exercise. Because of large individual differences it is difficult to individualise the advice. Perhaps the best guidance for athletes is to weigh themselves to assess fluid losses during training and racing and limit weight losses to 1% during exercise lasting longer than 1.5?h. Excessive fluid intake has been associated with hyponatremia. Post exercise the volume of fluid ingested and sodium intake are important determinants of rehydration.     

Cytokine responses to resistance training in people with multiple sclerosis

Abstract

Exercise for individuals with multiple sclerosis (MS) has been shown to improve cardiovascular function, increase strength and endurance, and reduce fatigue. The impact of exercise on immune function in the disease, however, remains mostly unexplored. Ten female MS patients participated in an 8 week programme of twice-weekly progressive resistance training, with pre- and post-training assessment of serum concentrations of cytokines IL-2, IL-4, IL-6, IL-10, CRP, TNF-α and IFN-γ. After training, IL-4, IL-10, CRP and IFN-γ showed statistically reduced resting concentrations in blood, while TNF-α showed non-significant reductions and IL-2 and IL-6 remained unchanged. These results suggest that progressive resistance training may have an impact on cytokine concentrations in individuals with MS and should be confirmed in studies with stronger statistical power. The impact of these changes on overall immune function in MS and on disease status and prognosis remains to be determined.