大豆多肽对过度训练大鼠肠道免疫功能的影响

目的:建立过度训练大鼠模型,探讨大豆多肽对过度训练大鼠肠道免疫功能的干预作用.方法:将大鼠随机分为对照组(C)、过度训练组(OT)和大豆多肽十预组(OS).榆测各组大鼠小肠组织SIgA、Gln的含量以及小肠CD4+、CD8+T细胞数量.结果:与对照组比较,过度训练组大鼠小肠组织中SIgA和Gln含量显著下降,CD4+T细胞数量、CD4+/CD8+比值显著下降,小肠CD8+T细胞数量显著升高;与OT组相比,CC4+T细胞数量、CD4+/CD8+的比值显著升高,CD8+T细胞的数量显著降低.结论:过度训练导致人鼠肠道免疫功能显著下降,过度训练时大鼠小肠Gln水平下降,可能是过度训练引起肠道免疫功能降低的主要机制.补充大豆多肽可显著改善长期人负荷运动训练大鼠肠道免疫功能,其确切机制还有待进一步深人研究.     

经导管肝动脉化疗栓塞(TAE)治疗肝癌的并发症及其防治(附50例分析)

本文对50例次经导管肝动脉化疗栓塞(TAE)治疗肝癌的并发症及其防治进行了分析。全部病例在TAE术后均有消化道症状,术后血清胆红质、尿素氮、丙氨酸氨基转移酶(ALT)和天门冬酸氨基转移酶(AST)均较术前有不同程度增高,重复化疗栓塞者易出现急性糜烂性胃炎,少见并发症有胆囊梗死、胸腔积液等。笔者还对部分并发症的防治进行了探讨。     

乳腺癌组织中c-erbB-2、p53和nm23蛋白表达及相互关系和意义

目的　探讨c erbB 2、p53和nm2 3蛋白在乳腺癌组织中的表达与乳腺癌临床病理参数、雌激素受体 (ER)、孕激素受体 (PR)的关系。方法　应用免疫组化ABC法对 5 5例乳腺癌组织中的c erbB 2、p53和nm2 3蛋白表达进行检测。结果　 5 5例乳腺癌中c erbB 2、p53及nm2 3的阳性率分别为 49 1 % (2 7/5 5 )、47 3 % (2 6 /5 5 )和 49 1 % (2 7/5 5 ) ,c erbB 2和p53蛋白在乳腺癌中的阳性率与肿瘤病理分级及淋巴结转移有显著意义 (P <0 0 5 ) ,与雌孕激素受体状况呈负相关 (P <0 0 1 )。nm2 3蛋白在乳腺癌中的阳性率与肿瘤病理分级、淋巴结转移有密切的关系 (p <0 0 5 ) ,与雌孕激素受体状呈正相关 (p <0 0 1 )。 70 9% (3 9/5 5 )肿瘤有上述蛋白的异常表达 ,其中 49 1 % (2 7/5 5 )的肿瘤同时有多个蛋白异常表达。结论　肿瘤的多因素分析比单因素分析更有价值 ,癌基因c erbB 2、nm2 3和抑癌基因p53蛋白的表达异常及协同作用在乳腺癌的发生发展中起重要使用     

结直肠癌营养风险筛查研究进展

营养风险是指现存的或潜在的与营养因素相关的导致患者出现不良临床结局的风险。结直肠癌是常见的恶性肿瘤,在我国发病率逐年升高,由于生理病理原因,以及治疗方法使其更易存在营养风险。现将营养风险及其筛查常用工具进行简单综述,并重点综述结直肠癌营养风险筛查2002（NRS 2002）的应用现状,以期指导临床实践。     

Derived vascular endothelial cells induced by mucoepidermoid carcinoma cells: 3-dimensional collagen matrix model

Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes, possibly due to proliferation of vascular endothelial cells (ECs) induced by mucoepidermoid carcinoma cells (MCCs) in their three-dimensional (3D) microenvironment. To date, no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model. In this context, the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs (MCMECs) conditioned by the microenvironment in the 3D collagen matrix model. We observed the growth of MCMECs purified by immunomagnetic beads and induced by MCCs, and characteristics of tubule-like structures (TLSs) formed by induced MCMECs or non-induced MCMECs. The assessment parameters involved the growth curve, the length, the outer and inner diameters, and the wall thickness of the TLSs, and the cell cycle. Results showed that MCCs induced formation of the TLSs in the 3D collagen matrix model. A statistically significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture (t=5.00, P=0.001). The outer and inner diameters (t ₁=5.549, P ₁=0.000; t ₂=10.663, P ₂=0.000) and lengths (t=18.035, P=0.000) of the TLSs in the induction group were statistically significant larger than those in the control group. The TLSs were formed at the earlier time in the induction group compared with the control group. It is concluded that MCCs promote growth and migration of MCMECs, and formation of the TLSs. The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma.     

Estrogen receptor expression in adrenocortical carcinoma

Objective: Adrenocortical carcinoma (ACC) is a rare but highly malignant tumor, and its diagnosis is mostly delayed and prognosis is poor. We report estrogen receptor (ER) expression in this tumor and our clinical experiences with 17 ACC cases. Methods: The data of the 17 patients (9 females and 8 males, age range from 16 to 69 years, mean age of 42.6 years) with ACC were reviewed, and symptoms, diagnostic procedures, treatment, and results of follow-up were evaluated. Immunohistochemistry was used to detect ER expression in tumor samples from the 17 patients. Results: At the time of diagnosis, 4 tumors were classified as Stage Ⅰ, 4 as Stage Ⅱ, 3 as Stage Ⅲ, and 6 as Stage Ⅳ. Eight patients demonstrated positive nuclear immunostaining of ER. The prognosis of patients with ER positive was significantly better (P<0.05) than that of patients with ER negative, with 1- and 5-year survival rates at 86% and 60% for ER-positive patients, and 38% and 0% for ER-negative patients, respectively. Conclusion: ER-positivity may be one of the factors associated with a worse prognosis of ACC.     

Liquid-based cytology aids in primary fallopian tube cancer diagnosis

Primary fallopian tube carcinoma (PFTC) is a rare malignant carcinoma among all genital tract malignancies. It occurs most commonly in postmenopausal women and is similar to ovarian malignancy historically and clinically. Because of its insidious onset and silent course, the diagnosis is made usually postoperatively. Liquid-based cytology (LBC) is a type of method for cervical cancer screening, but sometimes it may aid in making PFTC diagnosis. We report a 47-year-old woman with PFTC, whose diagnosis was made with the aid of LBC.      

运动干预调控肠道微生物介导认知功能的作用研究进展

认知功能障碍是神经系统疾病的重要特征表现,受诸多因素影响。肠道微生物在维持机体健康和多种疾病的发展中发挥作用,肠道微生物失衡与躯体运动能力和脑认知功能障碍,如阿尔茨海默病和帕金森病等神经退行性疾病关系密切。研究发现,运动干预可通过对肠道微生物的调节,建立肠-脑之间的联系,调控认知功能,改善神经退行性疾病,但不同方式的运动干预对肠道微生物的影响及其与认知功能的调节之间存在差异性。通过分析肠道微生物的生物学功能以及运动介导肠道微生物相关的改变与脑认知功能的关系,探讨肠道微生物在肠-脑神经联络以及运动调控肠道微生物改善认知功能的作用机理。     

中医药调控肝癌血管生成的研究近况

肝细胞癌（HCC）的发生与发展与血管形成有关,肝癌血管新生为肿瘤细胞提供生长和转移的条件。而血管生成因子和血管抑制因子是肝癌血管生成与否的物质基础,鉴于此,抑制血管生成因子的表达或者让血管抑制因子表达占优势,即可抑制肿瘤血管生成。目前临床上已逐步开展抗肝癌血管生成治疗。研究表明,很多中药具有抗肿瘤血管新生的作用。