Derived vascular endothelial cells induced by mucoepidermoid carcinoma cells: 3-dimensional collagen matrix model

Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes, possibly due to proliferation of vascular endothelial cells (ECs) induced by mucoepidermoid carcinoma cells (MCCs) in their three-dimensional (3D) microenvironment. To date, no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model. In this context, the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs (MCMECs) conditioned by the microenvironment in the 3D collagen matrix model. We observed the growth of MCMECs purified by immunomagnetic beads and induced by MCCs, and characteristics of tubule-like structures (TLSs) formed by induced MCMECs or non-induced MCMECs. The assessment parameters involved the growth curve, the length, the outer and inner diameters, and the wall thickness of the TLSs, and the cell cycle. Results showed that MCCs induced formation of the TLSs in the 3D collagen matrix model. A statistically significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture (t=5.00, P=0.001). The outer and inner diameters (t ₁=5.549, P ₁=0.000; t ₂=10.663, P ₂=0.000) and lengths (t=18.035, P=0.000) of the TLSs in the induction group were statistically significant larger than those in the control group. The TLSs were formed at the earlier time in the induction group compared with the control group. It is concluded that MCCs promote growth and migration of MCMECs, and formation of the TLSs. The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma.     

Liquid-based cytology aids in primary fallopian tube cancer diagnosis

Primary fallopian tube carcinoma (PFTC) is a rare malignant carcinoma among all genital tract malignancies. It occurs most commonly in postmenopausal women and is similar to ovarian malignancy historically and clinically. Because of its insidious onset and silent course, the diagnosis is made usually postoperatively. Liquid-based cytology (LBC) is a type of method for cervical cancer screening, but sometimes it may aid in making PFTC diagnosis. We report a 47-year-old woman with PFTC, whose diagnosis was made with the aid of LBC.      

Estrogen receptor expression in adrenocortical carcinoma

Objective: Adrenocortical carcinoma (ACC) is a rare but highly malignant tumor, and its diagnosis is mostly delayed and prognosis is poor. We report estrogen receptor (ER) expression in this tumor and our clinical experiences with 17 ACC cases. Methods: The data of the 17 patients (9 females and 8 males, age range from 16 to 69 years, mean age of 42.6 years) with ACC were reviewed, and symptoms, diagnostic procedures, treatment, and results of follow-up were evaluated. Immunohistochemistry was used to detect ER expression in tumor samples from the 17 patients. Results: At the time of diagnosis, 4 tumors were classified as Stage Ⅰ, 4 as Stage Ⅱ, 3 as Stage Ⅲ, and 6 as Stage Ⅳ. Eight patients demonstrated positive nuclear immunostaining of ER. The prognosis of patients with ER positive was significantly better (P<0.05) than that of patients with ER negative, with 1- and 5-year survival rates at 86% and 60% for ER-positive patients, and 38% and 0% for ER-negative patients, respectively. Conclusion: ER-positivity may be one of the factors associated with a worse prognosis of ACC.     

尿纤维连接蛋白在诊断上尿路移行细胞癌的临床意义

目的:探讨尿纤维连接蛋白(fibronectin,Fn)与上尿路移行细胞癌的生物学关系。方法:采用ELISA法检测30例上尿路移行细胞癌、25例其它泌尿系疾病患者及20例正常人尿液Fn含量,并同时测定尿肌酐(Creatin,Cr),计算Fn/Cr比值,比较各组人群尿Fn/Cr比值之间的差异;分析不同肿瘤分期、分级尿Fn/Cr之间的差异;比较其与尿脱落细胞学检测上尿路移行细胞癌的敏感性。结果:上尿路移行细胞癌尿Fn/Cr比值为(46.4±26.5)mg/mol,明显高于正常人(14.7±8.8)mg/mol及其它泌尿系疾病患者(28.2±22.7)mg/mol。以34mg/mol为标准,尿Fn/Cr比值诊断上尿路移行细胞癌的灵敏度和特异度分别为73.3%和77.8%。尿Fn/Cr比值与上尿路肿瘤分期分级有关。尿脱落细胞学检测上尿路移行细胞癌的敏感性为53.3%,采用Fn/Cr检测较脱落细胞学检查敏感性无统计学意义(P>0.05)。结论:尿Fn/Cr可作为上尿路移行细胞癌的诊断指标。其高低与膀胱癌的分期、分级有关,可大致判断浸润度和恶性度,并可用于上尿路移行细胞癌的预后评估。     

p53基因蛋白在食道正常上皮及增生、癌变后表达的观察

目的 :探讨p5 3蛋白在食管癌组织发生中表达的意义。方法 :应用免疫组织化学技术检测 72例食管鳞状上皮癌 ,19例增生鳞状上皮及 17例正常食道鳞状上皮中 p5 3蛋白的表达。结果 :p5 3在食管癌组织中的阳性率为 4 7.95 % (35 / 72 ) ,在增生鳞状上皮中的阳性率为 36 .84 % (7/ 19) ,在正常食道鳞状上皮中的阳性率为 5 .88% (1/ 17) ,正常组与增生组和癌变组比较均有显著性差异 (P <0 .0 5 )。p5 3蛋白与癌的分化程度、淋巴结有无转移和浸润深度等指标均无明显关系。结论 :p5 3蛋白与食管癌的发生、发展有关。     

Role of CD97stalk and CD55 as molecular markers for prognosis and therapy of gastric carcinoma patients

Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97^stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97^stalk was frequently observed in tumors of stage I and T1 gastric carcinoma patients. The expression of CD97^stalk between Stage I and Stage II, III, IV specimens showed significant difference (P<0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P<0.05). Specimens with tumor invasion depth limited in mucosa of T1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2 T3, T4 specimens was significantly different (P<0.05). There was strong correlation between the distribution patterns of CD97^stalk and CD55 on tumor tissues (r=0.73,P<0.05). Signet ring cell carcinomas frequently contained strong CD97^stalk and CD55-staining. Conclusions: Our results suggest that CD97^stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97^stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients. Project (No. 2004C34010) supported by the Science and Technology Bureau of Zhejiang Province, China     

GSTπ在膀胱移行细胞癌中的表达和意义

目的:探讨谷胱甘肽转移酶π(glutathione s-transferaseπ,GST-π)的表达与膀胱癌生物学行为的关系.方法:采用免疫组化方法对80例膀胱癌组织中GST-π进行观察.结果:GST-π总阳性率为66.25%(53/80),在Ⅰ级阳性率为45.45%(10/22),Ⅱ级中阳性率为71.43%(25/35),Ⅲ级中阳性率为78.26%(18/23).结论:GST-π的高表达与膀胱癌的病理分级有关,且可能与肿瘤的耐药有关.     

血粉、肉骨粉、肠衣粉替代进口鱼粉饲喂蛋雏鸡的研究

利用血粉、肉骨粉、肠衣粉等多种屠宰下脚料,按进口鱼粉能量和蛋白质水平所配制的代鱼粉料,等量替代进口鱼粉,对2000只12日龄的罗曼商品代蛋用雏鸡进行了饲养试验,代鱼粉料组和进口鱼粉组各1000只,试验结果表明;代鱼粉料组在增重、料肉比、成活率等方面都与进口鱼粉相接近,差异不显著(P>0.05),但每增加l公斤体重,比进口鱼粉组节约饲料成本0.19元,说明代鱼粉料等量全额替代进口鱼粉效果较好.     

Expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma and association with Epstein-Barr virus infection

Objective: To evaluate the expression of matrix metalloproteinase-9 (MMP9) in nasopharyngeal carcinoma and the association between MMP9 and Epstein-Barr virus infection. Methods: The MMP9 expression was studied by immu-nohistochemical analysis; and Epstein-Barr virus encoded small nuclear mRNA-1(EBER-1) produced by in situ hybridization were examined in 41 nasopharyngeal carcinoma sections, and the relation between them, and the associations of MMP9 with clinical features were statistically analyzed. Results: Positive expression rate of MMP9 was 73.17%. The expression of MMP9 showed significant positive correlation with the expression of EBER-1 (γ0.483, P=0.001). There was significant association of MMP9 expression with lymph nodes metastasis and clinical stage (P<0.001), non-significant association with age, gender, pathological classification and T classification. Conclusions: The highly pronounced expression of MMP9 is associated with cervical lymph nodes metastasis. Epstein-Barr virus can enhance N     

针灸治疗大肠癌术后30例疗效观察

[目的]观察针灸在大肠癌术后临床应用的疗效.[方法]将60例患者随机分为治疗组和对照组各30例,对照组采用西药口服治疗,治疗组采用针灸治疗.观察两组治疗后的效果,结果治疗组总有效率达96.67%,显效率为90%;而对照组有效率为40%,治疗组效果明显高于对照组.[结论]针灸在大肠癌术后具有很好的调节肠道功能的作用.