HDAC4在乳腺癌中的表达及临床病理学意义

目的：研究乳腺癌中HDC4基因蛋白的表达及其临床病理学意义。方法：实验共选取91例乳腺组织的存档蜡块标本,其中包括46例乳腺癌、26例导管内原位癌（DCIS）以及19例正常宫颈上皮组织。应用免疫组织化学（SP法）检测HDC4蛋白在上述组织中的表达,并分析其蛋白检测的临床病理学意义。结果：与正常乳腺组织（15.8%,3/19）相比,HDC4蛋白在DCIS和乳腺癌组织中表达率明显增高,分别达46.2%(12/26)和71.7%(33/46),强阳性表达率在DCIS和乳腺癌组织中分别达19.2%（5/26）和45.6%（21/46）,也明显高于正常乳腺组织,差异显著（p<0.01）。结论：HDC4蛋白表达可作为女性乳腺癌及原位癌DCIS的病理辅助诊断指标之一,可能有助于乳腺癌的诊断及预后评估。     

β-连环素与p63在大鼠舌部鳞状细胞癌癌变过程中作用的初步研究

[目的]研究β-连环素与p63基因在大鼠舌部鳞状细胞癌中的表达,探讨它们在口腔鳞状细胞癌增殖中的作用.[方法]免疫组织化学方法检测48例不同分化程度大鼠舌部鳞状细胞癌中β-连环素与p63基因的表达.[结果]8例高分化口腔鳞状细胞癌中分别有7和6例高表达p63与β-连环素.β-连环素和p63表达与大鼠舌部鳞状细胞癌的组织学分级不具有显著性差异(P>0.05).[结论]β-连环素在大鼠舌鳞状细胞癌增殖中的作用与在人回顾性研究中的作用相似.     

EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s

ＩＮＴＲＯＤＵＣＴＩＯＮＡｎｔｉｓｅｎｓｅｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅｏｔｉｄｅｓ (ＡＳＯＤＮ )ｃａｎｉｎｈｉｂｉｔｔｒａｎｓｃｒｉｐｔｉｏｎａｎｄｉｎｔｅｒｆｅｒｅｗｉｔｈｓｐｅ ｃｉａｌｇｅｎｅｅｘｐｒｅｓｓｉｏｎ .ＡｆｉｘｅｄｐｏｉｎｔｏｎｔｈｅＫ ｒａｓｇｅｎｅｗｉｔｈｈｉｇｈｍｕｔａｔｉｏｎｒａｔｅｉｎｐａｎｃｒｅａｔｉｃｃａｒｃｉ ｎｏｍａｏｆｆｅｒｓａｎｉｎｔｒａｃｅｌｌｕｌａｒｔａｒｇｅｔｆｏｒｐａｎｃｒｅａｔｉｃｃａｒｃｉｎｏｍａｇｅｎｅｔｈｅｒａｐｙｗｉｔｈＡＳＯＤＮ (Ｃａｉ…     

消化道穿孔合并复发性麻痹性肠梗阻X线征象分析

目的:对消化道穿孔并发症进行讨论。方法:对4例消化道穿孔合并麻痹性肠梗阻的患者进行透视,摄片定期检查。结果:保守治疗X线征象消失,但复查可见征象再现,再复查又可消失。结论:消化道穿孔合并麻痹性肠梗阻,保守治疗,其X线征象全部消失,不是临床治愈标准。     

Comparison of laparoscopic hepatectomy,percutaneous radiofrequency ablation and open hepatectomy in the treatment of small hepatocellular carcinoma

Objective

Three mainstream techniques—laparoscopic hepatectomy (LH), percutaneous radiofrequency ablation (pRFA), and open hepatectomy (OH)—were compared in this study, in terms of their efficacies in the treatment of small hepatocellular carcinoma (HCC).

Methods

A comparative study was performed within a total of 94 patients diagnosed with small HCC in our hospital from 2005 to 2010, who underwent LH (28), RFA (33), or OH (33). They had either a single tumor lesion of less than 5 cm or up to three nodules with diameters of less than 3 cm each. Outcomes were carefully evaluated throughout a 3-year follow-up interval and statistically interpreted.

Results

The pRFA group had a significantly lower disease-free survival rate compared with the two surgical groups (P=0.001) and significantly shorter overall survival (P=0.005), while the LH group and the OH group had no difference in survival results. For patients younger than 60 years old, surgical approaches offered a better long-term overall survival prognosis (P=0.008). There were no statistically significant differences among the three groups in overall survival for elderly patients (P=0.104).

Conclusions

Among patients with small HCC, LH may provide better curative effects than pRFA without increasing complication rates. pRFA leads to faster recurrence than surgical resections. LH has similar therapeutic effects to OH and causes less trauma. For patients younger than 60 years old, LH may be the best curative treatment. Elderly patients may choose either surgery or pRFA.

     

Effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s

Objective: To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Method: Human pancreatic cancer cell line PaTu8988s in exponential growth stage was used to study the effect of different drug concentrations on the cell line in the presence of different concentrations (0 μg/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml, 10 μg/m, 200 μg/ml) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out. Results: The inhibitory rate on the cell line PaTu8988s was 98.73%, 95.76%, 69.49%, 33.05% and 0 for ASODM concentrations of 200 μg/ml, 100 μg/ml, 50 μg/ml, 25 μg/ml and 5 μg/ml at 48 hours. Conclusions: K-ras complementary ASODN can inhibit the growth of human pancreatic cancer cell line PaTu8988s by 30.05% to 98.73%. This is likely to contribute to the specificity of the K-ras mRNA complementary capped ASODN sequential codon. Non-specific effect and side effect of ASOND were observed for instance, the greater the concentration is, the earlier the peak of inhibitory rate is reached. In concentration, of 25 μg/ml to 100 μg/ml ASODN showed a dose-effect correlation.     

幽门螺杆菌感染与胃粘膜萎缩肠化生及不典型增生的关系

对以上消化道症状而就诊的３２１例慢性胃炎患者行胃镜及幽门螺杆菌（ＨＰ）检测和胃粘膜病理学检查，结果显示慢性萎缩性胃炎的ＨＰ检出率为７６．１％，显著高于慢性浅表性胃炎的检出率５６．３％（Ｐ＜０．０１），ＨＰ感染患者胃粘膜萎缩和肠化生的发生率分别为２６．３％和２９．９％，显著高于无ＨＰ感染者胃粘膜萎缩和肠化生的发生率１２．６％及１９．７％（Ｐ＜０．０１和Ｐ＜０．０５）．且ＨＰ感染与胃粘膜萎缩程度有显著关系，而与肠化生程度和不典型增生无显著关系（Ｐ＞０．０５）．提示ＨＰ感染可以使胃粘膜萎缩和肠化生的发病率升高，并认为ＨＰ感染可能为胃癌发生的危险因素之一．     

Alteration of serum beta 2-microglobulin in oral carcinoma

Serum β₂-microglubin (β₂-m) levels were measured in oral carcinoma patients and compared with normal healthy controls. It was observed that there was a significant rise in serum β₂-microglobulin in oral carcinoma patients. Progressively higher values were obtained as the cancer advanced clinically. Therefore the estimation of serum β₂-microglobulin may be useful as one of a battery of tests in the assessment of oral carcinoma patients.     

涎腺粘液表皮样癌术后生存的临床病理因素分析

目的：探讨影响涎腺粘液表皮样癌患者术后生存的相关病理因素。方法：对98例涎腺粘液表皮样癌患者进行回顾性研究,分析影响患者术后生存的相关临床病理因素。通过SPSS10.0软件分析,筛选出影响粘液表皮样癌患者术后生存的主要临床病理因素。结果：与术后生存率相关的主要临床病理因素包括：临床分期、患者年龄、镜下是否发现坏死。结论：临床分期、患者年龄是影响涎腺粘液表皮样癌术后生存的主要临床因素,镜下坏死是影响涎腺粘液表皮样癌患者术后生存的主要病理因素。