泰山黄精对乌拉坦所致小鼠肺癌及抗炎作用研究

本文研究了泰山黄精对乌拉坦诱导的小鼠肺癌的作用及抗炎作用．方法：将昆明种小鼠随机分组并给予不同剂量的泰山黄精水煎剂,作用于鸟拉坦所致肺癌小鼠和二甲苯诱发耳肿胀小鼠。观察小鼠生存状况,记录其体重变化、内脏（胸腺、脾、肺）指数;记录小鼠耳片肿胀度．结果：泰山黄精可改善乌拉坦所致肺癌小鼠生存状态,提高了其内脏指数;同时能抑制二甲苯引起的小鼠耳肿胀．结论：泰山黄精对乌拉坦诱导的小鼠肺癌有一定的抑制作用且具有明显的抗炎作用．     

Adenovirus-mediated wild-type p53 gene transfer in combination with bronchial arterial infusion for treatment of advanced non-small-cell lung cancer, one year follow-up

Objective: In the present study, we have examined the safety and efficacy of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) injection in patients with advanced non-small-cell lung cancer (NSCLC) in the combination with the therapy of bronchial arterial infusion (BAI). Methods: A total of 58 patients with advanced NSCLC were enrolled in a non-randomized, two-armed clinical trial. Of which, 19 received a combination treatment of BAI and rAd-p53 (the combo group), while the remaining 39 were treated with only BAI (the control group). Patients were followed up for 12 months, with safety and local response evaluated by the National Cancer Institute's Common Toxicity Criteria and response evaluation criteria in solid tumor (RECIST), respectively. Time to progression (TTP) and survival rates were also analyzed by Kaplan-Meier method. Results: In the combo group,19 patients received a total of 49 injections of rAd-p53 and 46 times of BAI, respectively, while 39 patients in the control group received a total of 113 times of BAI. The combination treatment was found to have less adverse events such as anorexia, nausea and emesis, pain, and leucopenia (P<0.05) but more arthralgia. fever, influenza-like symptom, and myalgia (P<0.05), compared with the control group. The overall response rates (complete response (CR)+partial response (PR)) were 47.3% and 38.4% for the combo group and the control group, respectively (P>0.05). Patients in the combo group had a longer TTP than those in the control group (a median 7.75 vs 5.5 months, P=0.018). However, the combination treatment did not lead to better survival, with survival rates at 3, 6, and 12 months in the combo group being 94.74%, 89.47%, and 52.63%, respectively, com-pared with 92.31%, 69.23%, and 38.83% in the control group (P=0.224). Conclusion: Our results show that the combination of rAd-p53 and BAI was well tolerated in patients with NSCLC and may have improved the quality of life and delayed the disease progression. A further study to better determine the efficacy of this combination therapy is warranted.     

纳米氧化锌对人肺腺癌细胞A549的毒性

探讨纳米氧化锌（ZnO）对体外培养的人肺腺癌细胞A549的生物学效应。使用原子力显微镜（AFM）、透射电镜（TEM）和X射线衍射仪（XRD）研究纳米ZnO颗粒物的理化性质。然后,使用0mmol/L、0.1mmol/L、0.5mmol/L、1mmol/L、5mmol/L、10mmol/L的纳米ZnO处理体外培养的人肺腺癌A549细胞,MTT 法测定细胞生长活性。并且测定1mmol/L 纳米ZnO染毒24h后细胞培养液上清中,乳酸脱氢酶（LDH）和胞内的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性以及丙二醛（MDA）的含量。使用透射电镜和流式细胞仪检测细胞凋亡的情况。荧光染色检测细胞产生活性氧（ROS）的生成。实验所用的ZnO纳米颗粒为长75 nm、直径20nm的针状纤锌矿晶体。细胞实验结果表明,纳米ZnO颗粒对A549细胞的生长活性具有明显的抑制作用,存在剂量－效应关系。培养液上清中LDH活性显著升高（P < 0.05）,胞内CAT活性显著下降、MDA含量显著升高（P < 0.01）,但SOD活性下降不明显。荧光染色检测发现染毒后A549细胞出现细胞凋亡,而且细胞内ROS的生成与纳米ZnO存在剂量－效应关系。结论是纳米氧化锌诱导人肺腺癌A549细胞产生活性氧,引发细胞凋亡,并产生细胞毒性。     

Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF‍-κB pathway

Inflammation plays an important role in the development of acute lung injury (ALI). Severe pulmonary inflammation can cause acute respiratory distress syndrome (ARDS) or even death. Expression of proinflammatory interleukin-‍1β (IL-‍1β) and inducible nitric oxide synthase (iNOS) in the process of pulmonary inflammation will further exacerbate the severity of ALI. The purpose of this study was to explore the effect of Palrnatine (Pa) on lipopolysaccharide (LPS)-induced mouse ALI and its underlying mechanism. Pa, a natural product, has a wide range of pharmacological activities with the potential to protect against lung injury. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed to detect the expression and translation of inflammatory genes and proteins in vitro and in vivo. Immunoprecipitation was used to detect the degree of P65 translocation into the nucleus. We also used molecular modeling to further clarify the mechanism of action. The results showed that Pa pretreatment could significantly inhibit the expression and secretion of the inflammatory cytokine IL-1β, and significantly reduce the protein level of the proinflammatory protease iNOS, in both in vivo and in vitro models induced by LPS. Further mechanism studies showed that Pa could significantly inhibit the activation of the protein kinase B (Akt)/nuclear factor-κB (NF-κB) signaling pathway in the LPS-induced ALI mode and in LPS-induced RAW264.7 cells. Through molecular dynamics simulation, we observed that Pa was bound to the catalytic pocket of Akt and effectively inhibited the biological activity of Akt. These results indicated that Pa significantly relieves LPS-induced ALI by activating the Akt/NF-κB signaling pathway.     

有氧运动改善哮喘儿童运动能力和生活质量的实验研究*

目的：观察3个月有氧运动对哮喘儿童肺功能、运动能力和生活质量的影响,探讨运动康复在哮喘管理中的作用。方法：40名哮喘儿童随机分为运动（E）组（n=20）和对照（C）组（n=20）,E组进行3个月中等强度有氧运动,C组保持日常生活习惯不变。实验前后分别测定受试者的肺功能、运动能力和生活质量,实验过程中记录运动情况和不良反应,实验后调查家长满意度。结果：E组运动时的不良反应率为2.6%,家长满意度为95.0%。实验后,组内与实验前比较,E组6 min步行实验（6MWT）后的步行距离,生活质量问卷总评分与各分项评分增加（P<0.05）,6MWT后HR、SBP、MAP和RPE降低（P<0.05）,C组生活质量问卷中症状评分增加（P<0.05）;组间比较,E组上述各指标与C组均存在统计学差异（P<0.05）;肺功能参数在两组组内与组间比较均无统计学意义（P>0.05）。结论：在适当管理和监控下,中等强度有氧运动具有良好的安全性和有效性,可改善哮喘儿童运动能力和生活质量。     

应用流式细胞术检测同步化处理的肺癌细胞系细胞周期

本文应用吖啶橙酸变性染色方法流式细胞术检测经5-氟脱氧尿嘧啶核苷同步化技术处理的肺癌细胞系GLC-82和HAC-84的细胞周期。结果显示经同步化处理的肿瘤细胞M期比例明显增加,且M期细胞比例与同步化后处理时间密切相关。     

电视胸腔镜下行肺大泡手术的麻醉处理

目的:研究电视胸腔镜下肺大泡手术的麻醉处理。方法:择期手术病人10例,ASAⅠ～Ⅱ级。芬太尼、氟哌啶、异丙酚、琥珀胆碱诱导后,插入双腔气管导管,吸入2％～3％异氟醚及维库溴胺维持麻醉。肺大泡钳夹过程中行健侧单肺通气。监测Bp、HR、SpO_2、ECG、ETCO_2及血气分析。结果:全组病人术中Bp、HR平稳,单肺通气期间SpO_2 99％～100％,血气分析各参数正常。结论:肺大泡手术的病人选用异氟醚及维库溴胺静脉吸入复合麻醉,能够保证单肺通气期间电视胸腔镜下行肺大泡手术的要求。     

不同体育锻炼项目对老年人心肺功能的影响

运用问卷调查法和实验法相结合的研究方法,对参加不同体育锻炼项目的720例湖北省老年人(60-69岁)心肺功能指标进行测试。研究表明:不同体育锻炼项目对湖北省老年人收缩压、脉压差和肺活量测试指标具有显著影响(p<0.05)。武术、健身气功项目对提高老年人的心血管系统功能具有积极作用,球类项目对改善老年人呼吸系统功能具有积极影响。     

析《文心雕龙》"体"的文论范畴

“体”是《文心雕龙》中重要的文论概念，具有十分丰富复杂的内涵，是一个概念系统，涉及《文心雕龙》一书的文章学理论的诸多方面的问题。综合来看，“体”主要包括有“体”（体类）、“体要”、“体貌”、“体性”、“体势”等不同的含义，这些既相互联系又相互区别的“体”的复杂观念共同构成了“体”的意义系统，表述了刘勰关于“体有六义”的文论思想。     

Physical Characteristics and Oxygen Utilization of Male and Female Marathon Runners

Verbal and nonverbal communication is a critical mediator of performance in team sports and yet there is little extant research in sports that involves direct measures of communication. Our study explored communication within NCAA Division I female tennis doubles teams. Video and audio recordings of players during doubles tennis matches captured the communications that took place between and during points. These recordings were coded and sequential analysis computed using the Discussion Analysis Tool software (Jeong, 2003). Results indicated that most communications were emotional (i.e., > 50%) or action statements (i.e., > 25%). Winning teams exhibited significantly different communication sequences than losing teams. In particular, winning teams had a more homogeneous model of communication, which perhaps makes message interpretation more reliable. Finally, winning teams exchanged twice as many messages as losing teams.