多重PCR技术在植物学研究中的应用

多重PCR是近几年新兴的一项分子生物学技术,能够同时扩增不同片段.具有高特异性、高灵敏度、高效率、低成本的特点,有普通PCR方法不可比拟的优势.简述了多重PCR技术在植物学研究中的应用现状,分析了影响其扩增效果的因素,并介绍了多重PCR的应用前景.     

略论90年代先锋诗歌的文化境遇与多元流向

本文对20世纪90年代先锋诗歌的文化境遇进行了简要的描述,在此基础上,对90年代先锋诗歌的内部演化状况及先锋诗歌写作的多元流向与审美格局予以了富有个性的描述与阐析.     

浅析波分复用WDM系统

以波分复用(WDM)技术的原理为基础,分析了信号频带、信号的放大和非线性困扰,以及波长变换技术。得出了要实现宽频带、多信号的较好传输信号,必须考虑放大器的增益平坦、噪声以及WDM系统的非线性,而且信号间的畅通传送,波长变换技术也是必不可少。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients withHelicobacter pylori infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) andHelicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status ofH. pylori was determined by histologic examination, RUT,¹⁴C-UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients withH. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml,P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection ofH. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml, respectively (P<0.05). The serum levels of sICAM-1, proved to be significantly correlated with the density ofH. pylori colonization in gastric mucosa (r _s=0.316,P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated thatH. pylori infection up-regulates the expression of sICAM-1.     

法制探索与明清长篇小说的主题表现

明清长篇小说中，有不少涉及到法制问题。尽管有的小说不是纯粹的法制小说，但法制探索是其主题表现的一个重要组成部分。在古代法制文学中，有许多重大而深刻的法制主题，如平等主题、警世主题、复仇主题、冤屈主题等，有的作品则集多个法制主题于一身，形成多元建构的特征。深入研究明清长篇小说中的法制问题及其表现艺术，对于探索中国法制文学的发展历程有着重大的意义。     

分层多元化的高职实践教学质量监控与评价体系构建

高职人才培养质量的关键在于实践教学。针对我国高职实践教学质量监控和评价的现状及问题,探讨了高职实践教学质量监控和评价体系的目标和构建原则,提出了包括政府、学校、社会在内的"分层多元化"高职实践教学质量监控和评价体系总体构架与主要内容。     

体育产业治理模式的选择与评析

体育产业治理存在传统政府治理、市场主导型治理、体育行业协会治理、体育企业或集团自我控制治理等多种治理模式,每种模式都有优点,也存在诸多缺点。本文提出新时期我国的体育产业治理的三种模式选择,并指出多元化协同模式为其最佳,即政府、市场和以体育行业协会、体育中介为主的非政府组织以及企业等多元因素相互协同起作用的模式,并通过体育产业多元化协同治理与传统的政府治理、市场治理的比较指出了体育行业协会在多元化协同治理中的突出地位。     

A rapid method for plasma ammonia estimation using an indigenously purified enzyme

Determination of ammonia level in blood is important, especially in the diagnosis of hepatic disorders. An indigenously purified enzyme was used in the standardisation of the assay. The assay is a two reagent system, requires five minutes for completion and can be performed at temperature between 25–27°C. Performance of the assay was assessed by linearity, imprecision, functional sensitivity and interference studies. Lyophilised reagent I and reagent II were found stable for at least one year. The plasma level of ammonia for the controls was 13.7±7.3 μMol/L, whereas for subjects of hepatic disorders, it was 69.1±32.4 μMol/L (P<0.001). The functional sensitivity was between 2–1000 μMol/L. Within-run coefficient of variation was between 1.1–2.0% and between-run coefficient of variation was between 1.9–3.7%. The mean recovery after dilution was 99.6%. The present method can estimate ammonia up to 1000 μMol/L without dilution of sample. Assay time of five minute may be shortened to one minute. This method is suited for routine clinical use in treatment of hepatic disorders.     

SRB显色法用于抗癌药物敏感性试验的研究

采用ＳＲＢ显色法对９６孔培养板中短期培养的人大肠癌细胞株Ｃｏｌｏ２０５、人骨肉瘤细胞株ＯＳ７３２,人舌癌细胞株Ｔｃａ８１１３经５－ＦＵ,顺铂,蟾酥等药物处理后的活性进行了研究。结果表明,ＳＲＢ法对这些细胞株的体外抗癌药物敏感试验结果稳定,该法与其它细胞毒试验相比有一定优点,值得推广应用。     

校内"四位一体"实践教学基地的建设

在总结实践教学基地建设的经验基础上,提出在高职教育校内实训基地建设中坚持产、学、研、培"四位一体"的建设指导思想可使我们获得多渠道的资金来源,形成滚动发展的良性循环;有利于发挥基地潜力,获得一定的社会和经济效益,并进一步促进基地的发展;可获得高度仿真的实训环境,有利于缩短校企距离,增强学生快速适应社会的能力。