榆林市中学生利用多元体育资源进行体育锻炼的现状分析

笔者从榆林市中学生利用多元体育资源进行体育锻炼的现状入手,对影响中学生利用多元体育资源进行体育锻炼的诸多因素进行探索和研究,分析其现今存在的缺陷,并提出相关建议,以更好的促进榆林市中学生体育水平的提高及终身体育习惯的养成。     

History-dependent percolation on multiplex networks

The structure of interconnected systems and its impact on the system dynamics is a much-studied cross-disciplinary topic. Although various critical phenomena have been found in different models, study of the connections between different percolation transitions is still lacking. Here we propose a unified framework to study the origins of the discontinuous transitions of the percolation process on interacting networks. The model evolves in generations with the result of the present percolation depending on the previous state, and thus is history-dependent. Both theoretical analysis and Monte Carlo simulations reveal that the nature of the transition remains the same at finite generations but exhibits an abrupt change for the infinite generation. We use brain functional correlation and morphological similarity data to show that our model also provides a general method to explore the network structure and can contribute to many practical applications, such as detecting the abnormal structures of human brain networks.     

论立体农业

文章依据立全思维的要求并响应农学家们关于建构立体农业的“倡议”，从理论上论述了立体农业的必要性与可行性，并设计了立体农业若干种模式，指出立体农业在人多地少的我国乃是农业的根本出路。     

管碟法研究臭牡丹提取物抑菌活性

采用管碟法研究臭牡丹根和茎的水提取物对四种试用菌的抑菌效果.结果表明臭牡丹提取物对大肠杆菌、枯草杆菌、金黄色葡萄球菌和放线菌均具有不同程度的抑菌作用,抑菌效果随提取液浓度的加大而增强.根提取物的抑菌效果总体比茎好.大肠杆菌、枯草杆菌在浓度为8.0mg/mL时具抑菌效果,而放线菌和金黄色葡萄球菌在浓度为4.0mg/mL具抑菌效果.根提取物对金黄色葡萄球菌、大肠杆菌的抑菌能力随着pH的增大而减小;放线菌则相反.     

Multiplex PCR for rapid detection of exonal deletions in patients of duchenne muscular dystrophy

Duchenne muscular dystrophy (DMD) is the most common X-linked disorder in children affecting 1 in 3500 males. Since, as of now, we have no treatment for DMD, carrier detection and prenatal diagnosis is the most important preventive strategy. Multiplex PCR helps in rapid detection of hot spot exonal deletions (positive in 65% of cases) as many exons can be identified in a single run. 10 children with characterstic clinical features of DMD and chorionic villus samples of 10 antenatal patients with positive family history were studied. We identified a deletion mutation in exon 49 of the dystrophin gene in a 4 yr old boy referred with signs and symptoms suggestive of DMD using primers for exons 45, 48, 49, 43, 44, 19, 3, 8, 13 and muscle promoter, subjected to multiplex polymerase chain reaction (PCR) and agarose/Nu-Sieve gel electrophoresis. These genetic methods aid in prenatal diagnosis of DMD as well as confirmation of diagnosis in children with signs and symptoms suggestive of the disease. Work done as WHO fellow in Deptt. of Genetics, All India Institute of Medical Sciences, New Delhi.     

伤寒免疫学诊断方法的研究现状

对近年伤寒免疫学诊断方法结合作者的研究工作进行了评价。伤寒免疫学诊断可分检测早期病人体液中抗原及测定患者血清中抗体两个方面。检测抗原以SPA-CoA与ELISA(包括Dot-ELISA)较为敏感和特异,而DRLAT则有简便的优点。测定抗体以PHA最为简便与快速。使用LPS-PHA可作伤寒的早期快速诊断,而Vi-PHA可作伤寒病后带菌者的初筛。ELISA有较PHA更高的敏感性,其中LPS-IgM-ELISA亦可作伤寒病人的早期诊断,而LPS-IgG-ELISA可用于该病的追溯诊断和血清流行病学调查。Vi-ELISA还可作伤寒病后带菌者初筛。不同条件实验室可根据取得试剂情况选择使用。     

异烟肼片含量测定方法的改进

以甲基橙代替乙氧基黄叱精指示剂，用溴酸钾法测定异烟肼含量，讨论了温度、被测液酸度、体积、滴定速度等因素对测定结果的影响。测得异烟肼平均回收率为１００．０８％，ＲＳＤ＝０．２４％。经与九○年版中国药典法比较，所得结果一致，本法具有终点易观察，滴定操作时不必控制温度等优点。     

Enantioselective assay of S(+)- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization

The enantioselective assay for S( )- and R(-)-propafenone (PPF) in human urine that developed in this work involves extraction of propafenone from human urine and using S( )-propafenone as internal standard, chiral derivatization with 2,3,4,6-tetra-O-β-D-glucopranosyl isothiocyanate, and quantitation by an RP-HPLC system with UV detection (λ=220nm). A baseline separation ofpropafenone enantiomers was achieved on a 5-μm reverse phase ODS column, with a mixture of methanol:water:glacial acetic acid (25:12:0.02,v/v) as mobile phase. There was good linear relationship from 24.9 ng/ml to 1875.0 ng/ml for both of enantiomers. The regression equations of the standard curves based on Cs-PPF (or CR-PPF ) versus ratio of As-PPF/As (or AR-PPF/As ) were y=0.0032x-0.081, (r=0.999) for S-PPF and y=0.0033x 0.0039, (r=0.998) for R-PPF,respectively. The method's limit of detection was 12.5 ng/ml for both enantiomers, and the method's limit ofquantitation was 28.2±0.52 ng/ml for S-PPF, 30.4±0.53 ng/ml for R-PPF (RSD＜8%, n=5). The analytical method yielded average recovery of 98.9% and 100.4% for S-PPF and R-PPF, respectively. The relative standard deviation was no more than 6.11% and 6.22% for S-PPF and R-PPF, respectively. The method enabled study of metabolism of S( )- and R(-)-propafenone in human urine. The results from 7 volunteers administered 150 mg racemic propafenone indicated that propafenone enantiomers undergo stereoselective metabolism and that in the human body, S( )-propafenone is metabolized more extensively than R(-)propafenone.     

魔芋甘露聚糖的性质测定及应用

文中对魔芋甘露聚糖的性质和应用进行了较为详细的介绍,对测定魔芋甘露聚糖的两种方法即甘露脎法和分光光度法,从原理、水解、操作要点、计算方法和优缺点等方面进行了系统的对比,阐明了两种方法的主要区别和实际操作中应注意的有关问题。