异烟肼片含量测定方法的改进

以甲基橙代替乙氧基黄叱精指示剂，用溴酸钾法测定异烟肼含量，讨论了温度、被测液酸度、体积、滴定速度等因素对测定结果的影响。测得异烟肼平均回收率为１００．０８％，ＲＳＤ＝０．２４％。经与九○年版中国药典法比较，所得结果一致，本法具有终点易观察，滴定操作时不必控制温度等优点。     

伤寒免疫学诊断方法的研究现状

对近年伤寒免疫学诊断方法结合作者的研究工作进行了评价。伤寒免疫学诊断可分检测早期病人体液中抗原及测定患者血清中抗体两个方面。检测抗原以SPA-CoA与ELISA(包括Dot-ELISA)较为敏感和特异,而DRLAT则有简便的优点。测定抗体以PHA最为简便与快速。使用LPS-PHA可作伤寒的早期快速诊断,而Vi-PHA可作伤寒病后带菌者的初筛。ELISA有较PHA更高的敏感性,其中LPS-IgM-ELISA亦可作伤寒病人的早期诊断,而LPS-IgG-ELISA可用于该病的追溯诊断和血清流行病学调查。Vi-ELISA还可作伤寒病后带菌者初筛。不同条件实验室可根据取得试剂情况选择使用。     

Antinuclear Antibody Profiling in Patients of a Tertiary Care Centre in Central India

The incidence of autoimmune disorders that includes the connective tissue diseases has seen a rise in India in recent times. Antinuclear antibodies, the telltale sign of systemic autoimmune response, thus can be used as a screening tool and also to support the diagnosis of systemic autoimmune disease. The present retrospective cross- sectional analysis aimed to study the antinuclear antibodies profile (patterns and specific antibody reactivity) amongst suspected cases of auto-immune disorders at a tertiary care teaching hospital. The study retrieved and reviewed reports of 644 patients sent for ANA testing by indirect immunofluorescence assay over a period of 1 year by different specialty departments. Positive samples were further processed for anti-ds-DNA antibody and antibodies to extractable nuclear antigen. Data collected was statistically analysed. ANA pattern positivity was observed in 31% of cases and a positive antibody reactivity was seen in 66% of them. Female predominance (82%) was noted in both pattern positivity and antibody reactivity. High levels of pattern positivity and antibody reactivity was found in the young adults (45.9%). Amongst the ANA patterns, the nuclear homogenous pattern was found the commonest. The common antibodies associated with this pattern were anti-dsDNA and U1 Sm/RNP antibodies. A stronger fluorescence intensity on initial screening showed a higher confirmation rate for specific antibodies on immunoassay. High occurrence of positive ANA patterns in autoimmune disorders suggests its utilization as a screening tool for them and would also play an adjuvant to the diagnosis. Early knowledge about future autoimmunity will earn better prognostic achievements through better treatment interventions.     

高校基本建设融资管理探讨与实践

分析了高校基本建设的多元化融资渠道及各融资方式的特点与适用性,结合实际案例进一步探讨了适合高校基本建设的融资模式。通过分析认为,依靠政府拨款和利用自有资金难以满足高校基本建设的需要,高校应更多地探讨外部融资渠道,包括发行教育债券、资产置换、与地方政府合作、与企业合作等,以求促进高校基本建设的可持续性发展。     

片形吸虫成虫虫体抗原制备及ELISA实验研究

目的：研究人体片形吸虫病ELISA检测试剂盒的检测效果。方法：采用云南大理本地采集的牛体片形吸虫成虫制备可溶性粗抗原,包被反应板,采用人体片形吸虫病ELISA法（FAWA-ELISA）进行研究,分别检测26份片形吸虫患者血清、102份其他寄生虫病患者血清和25份健康人血清,评价检测试剂盒的检测效果。结果：检测试剂盒的敏感性为100.0%、特异性分别为95.28%（95%CI：98.9%～91.2%）,约登指数分别为0.95。结论：FAWA-ELISA试剂盒检测效果较好,且成本相对较低,可适宜用于云南片形吸虫病流行区流行病学筛查,减轻病原学检测的工作量。