工程硕士培养及管理特点分析

工程硕士的培养目标是培养工程实践者。社会经济的发展催生了工程硕士这一教育形式的产生,高等院校顺应社会发展需要,针对工程硕士培养的特点,培养工程技术领域掌握前沿科学知识同时具有综合管理素质的高层次、复合型人才。为在职人员提供进一步深造的机会同时满足社会对高素质人才的需求。     

Binding of circulating autoantibodies in breast cancer to native and peroxynitrite-modified RNA

Peroxynitrite(ONOO) is a powerful oxidant and nitrosative agent and has in vivo existence.The half life of ONOO at physiological pH is less than 1 s.It can react with nucleic acids,proteins,lipoproteins,saccharides,cardiolipin,etc.,and can modify their native structures.Action of ONOO,synthesized in the authors’ laboratory by a rapid quenched flow process,on structural changes of commercially available RNA was studied by ultraviolet(UV),fluorescence,and agarose gel electrophoresis.Compared to native RNA,the ONOO-modified RNA showed hyperchromicity at 260 nm.Furthermore,the ethidium bromide(EtBr) assisted emission intensities of ONOO-modified RNA samples were found to be lower than the emission intensity of native RNA-EtBr complex.Agarose gel electrophoresis of ONOO-modified RNA showed a gradual decrease in band intensities compared to native RNA,an observation clearly due to the poor intercalation of EtBr with ONOO-modified RNA.Native and ONOO-modified RNA samples were used as an antigen to detect autoantibodies in sera of patients with clinically defined breast cancer.Both direct binding and inhibition enzyme-linked immunosorbent assay(ELISA) confirmed the prevalence of native and 0.8 mmol/L ONOO-modified RNA specific autoantibodies in breast cancer patients.Moreover,the progressive retardation in the mobility of immune complexes formed with native or 0.8 mmol/L ONOO-modified RNA and affinity purified immunoglobulin G(IgG) from sera of breast cancer patients supports the findings of the direct binding and inhibition ELISAs.The peroxynitrite treatment to RNA at a higher concentration appears to have damaged or destroyed the typical epitopes on RNA and thus there was a sharp decrease in autoantibodies binding to 1.4 mmol/L ONOO-modified RNA.It may be interpreted that cellular nitrosative stress can modify and confer immunogenicity on RNA molecules.Higher concentrations of nitrogen reactive species can be detrimental to RNA.However,the emergence of native as well as 0.8 mmol/L ONOO-modified RNA as a novel antigen/substrate for autoantibodies in breast cancer patients indicates that,in future,these molecules might find a place on the panel of antigens for early diagnosis of breast cancer.     

兴奋剂检测方法的研究进展

目前常用的兴奋剂检测方法有气相色谱、高效液相色谱、气相色谱-质谱联用、液相色谱-质谱联用、化学发光免疫分析法和电化学法等。色谱-质谱联用技术仍是兴奋剂检测的主要方法,气相色谱-质谱法和液相色谱-质谱法以及不断衍生的各类质谱联用技术可被用于检测大部分兴奋剂。免疫分析法主要被应用于类固醇类兴奋剂和激素类兴奋剂的检测。电化学法只能针对具有特定电活性的物质进行检测。基因兴奋剂的检测仍处在初级阶段。     

"EDA技术"课程实践教学环节的改革与探索

“EDA技术”是高校信息类专业一门实践性、应用性很强的课程。“EDA技术”课程实践教学环节存在着弊端,在教学实践中应采取“开展多元化的实践教学模式”、“提倡学生课外实践使用硬件核心开发板”等措施,以提高学生应用EDA技术设计数字系统的能力。     

论兴隆洼文化在中国文明起源中的地位与作用

兴隆洼文化是中国东北地区距今8000年的重要考古学文化,其文化内涵深邃厚重,与中华文明起源密切相关。在复合型经济形态和大规模定居基础上发展起来的尚玉礼俗、崇龙现象及祖先崇拜等,构成了中华文明起源的核心因素。     

农村纠纷解决机制初探

在当代中国农村,如果解决好广泛存在的社会冲突,不仅直接影响农村的稳定,也影响到党中央提出的建设社会主义新农村战略的具体实施,从农村社会纠纷解决的现实出发,指出由于传统文化的影响,农村社会的特点等多方面的原因决定了农村社会的纠纷解决机制不能单纯的依靠法治这一惟一的路径,必须考虑农村社会的客观现实,构建多元化的纠纷解决机制。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

冠心病患者及健康人血浆脂蛋白(a)水平

本文以酶联免疫吸附法(ELISA)测定了54例不同类型及不同并发症的冠心病患者与30名健康人的血浆Lp(a)浓度。心肌梗塞组和心绞痛组的Lp(a)浓度均高于对照组,并发糖尿病组Lp(a)浓度显著高于其他各组,无并发症且年龄较轻者Lp(a)浓度显著高于年龄较高者。表明血浆高Lp(a)水平是冠心病的遗传危险因素,但其升高的程度不能反映冠心病的临床类型或严重程度;并发糖尿病是老年冠心病患者血浆Lp(a)浓度升高的原因之一。     

任职教育“多元化”教学模式探讨

"多元化"教学是具有现代意识的一种教学模式,它以培养创新精神和实践能力为重点,对提高人才培养质量具有十分重要的实践价值。本文结合专业教学实践,就以"多元化"教学为基本模式,提高任职教育模式下人才培养质量的有关问题进行了初步探讨。