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31.
Mcm7是微小染色体维持蛋白家族成员之一,与其他的Mcm蛋白形成异多聚体,调节DNA的复制。它与转录成分关联,可能与RNA合成即转录有关,并且增殖细胞或组织高表达Mcm7,可以作为肿瘤生物标志的候选因子。 相似文献
32.
INTRODUCTION Bone morphogenetic proteins (BMPs) are agroup of proteins that can induce new bone forma-tion at orthotopical and heterotopical sites in ex-perimental animals (Urist et al., 1983; Groeneveldand Burger, 2000). Their biological activity hasstimulated interest in its clinical use for bone repair.In clinical use, BMPs are undergoing evaluationincluding system administration, gene transfer andlocal matrix delivery vehicle implantation (Kirker-Head, 2000; King et al., 199… 相似文献
33.
34.
脂肪酸的从头合成主要发生在肝脏和脂肪组织,肝脏脂肪酸的合成受到多种因素的调节,文章重点介绍调节脂肪酸合成的核转录因子:肝脏X受体固醇调节元件结合蛋白、碳水化合物反应元件结合蛋白。 相似文献
35.
Li-rong Chen Hong-wei Zhou Jia-chang Cai Rong Zhang Gong-xiang Chen 《Journal of Zhejiang University. Science. B》2009,10(5):348-354
Objective To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants
qnr and aac(6′)-Ib-cr in a clinical isolate of Enterobacter cloacae.
Methods An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic
susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain
reaction (PCR), and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed
to confirm the genotype. Outer membrane proteins (OMPs) were examined by urea-sodium dodecyl sulfatepolyacrylamide gel electrophoresis
(Urea-SDS-PAGE).
Results Minimum inhibitory concentrations (MICs) of imipenem, meropenem, and ertapenem for ZY106 were 2, 4, and 16 μg/ml, respectively.
Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and
CTX-M-3 extended-spectrum β-lactamase, and E. coli transconjugant produced IMP-1. Plasmid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrS1-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZY106 lacked an OMP of approximately 38 kDa.
Conclusion It is the first IMP-1-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blaIMP and qnrS genes as well. The blaIMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance
and reduced imipenem and meropenem susceptibility in E. cloacae.
相似文献
36.
Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimethylphenol:57.5% sulphuric
acid conventional method and the values were 0.39±025 and 0.69±0.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively.
The mean increase in glycated hemoglobin values in diabetics (1.8fold) was highly significant (p<0.001). A good correlation
(r=0.95) was found between the glycated hemoglobin values obtained by this method and the phenol:sulphuric acid method. The
values obtained by former method were about 1.2–1.4 times the values by the phenol:sulphuric acid method. This study indicates
that conventional 2,6-dimethylphenol: 57.5% sulphuric acid method is more sensitive for the estimation of glycated hemoglobin
than any other method based on the same principle. It is less time consuming, reliable and hence can be employed for the routine
laboratory estimation of glycated hemoglobin for the assessment of glycemic control. 相似文献
37.
小麦胚生物活性肽产品开发研究进展 总被引:1,自引:0,他引:1
小麦胚芽中不但富含维生素E、维生素B等多种B族维生素,还含有膳食纤维、矿物质和活性多肽等生物活性物质,已成为当前食品工业中新产品开发的重要方面.本文阐述了小麦胚生物活性肽的营养价值和小麦胚多肽产品的开发研究现状.旨在为进一步开发小麦胚多肽产品提供参考. 相似文献
38.
Membrane lipid and protein composition was compared in erythrocytes from iron deficiency anemia (IDA) and heterozygous beta
thalassemia patients. The study was planned to correlate the influence of iron deficiency with the intrinsic defect of the
heterozygous condition on the membrane structural integrity as well as to investigate whether there are differences in membrane
changes between the two conditions. Results indicate high levels of saturated fatty acids and low unsaturated fatty acids
in both disorders although arachidonic acid and the unsaturation index were lower in heterozygous thalassemia than IDA. Nevertheless,
neither of the conditions provoked any alterations in membrane protein or glycophorin suggesting alterations in the lipid
moiety only. Present findings indicate that irrespective to the etiology, both, iron deficiency and the heterozygous condition
show a common pattern of lipid derangement, which may in turn result in increased membrane rigidity and decreased cellular
deformability. 相似文献
39.
黄晓冬 《泉州师范学院学报》2001,19(4):67-70
近些年研究表明,保卫细胞质膜上存在多种转运蛋白,这些特殊结构是保卫细胞离子出入,信号导等生理过程的重要调控机构。研究保卫细胞质膜转运蛋白特性、功能与气孔运动的关系对于认识气孔运动机理,调控气孔运动等有重要意义。 相似文献
40.
通过低温处理小麦幼苗来诱导抗冻蛋白的表达,采用DEAE-52和丙烯葡聚糖S300柱层析的方法分离了小麦幼苗中的抗冻蛋白,并对所分离的蛋白进行了SDS-PAGE分析,而且对其抗氧化活性也进行了初步研究,结果表明:低温处理可以诱导小麦幼苗抗冻性,从小麦幼苗中分离到一类热稳定的抗冻蛋白,其分子质量约为20~34ku,当浓度为10g/L时,测得其热滞活性为0.3℃.此类蛋白对羟基自由基活性有一定的抑制作用,表明它具有抗氧化活性. 相似文献