大负荷专项训练对散打运动员铁代谢影响的研究

本研究采用生化测定方法检测受试者在一次大负荷散打专项训练前后血清铁含量的变化,分析探讨一次大负荷专项训练对不同运动水平男子散打运动员铁代谢的影响。结果表明:一次大负荷散打专项训练可引起运动员血清铁含量显著降低。     

大学男生运动前、后唾液与血清尿素氮、肌酐、尿酸含量的检测

为了探讨运动对唾液成分含量变化的敏感效应,并与血清作比较.以江西医学院二年级男生10例、年龄18～21岁、均体检为身体健康者为研究对象,于早晨空腹运动(中速,800 m)前、后分别采集唾液和血样,进行处理后,用全自动生化分析仪进行测定.结果发现:1)运动后,唾液尿素氮含量如同血清具有显著升高(P＜0.001).2)运动后,唾液和血清的肌酐含量有下降倾向,原因尚待探讨.3)运动后,唾液与血清的尿酸含量均呈极显著升高(分别为P＜0.001及P＜0.005).表明:唾液中以上三种成分的含量变化与血清类同,而且尿酸或许可作为体育运动的一个检测指标,值得进一步探讨.     

不同赛期优秀足球运动员血红蛋白、血清睾酮的变化

采用跟踪实验法,对国内甲A足球队在联赛前、联赛中调整期和赛事密集期3个不同时期的Hb、T的跟踪测试．发现不同赛期的Hb、T呈相应的变化,提示Hb、T作为运动员机能状态监控的参考指标具有一定的实用价值;T和Hb有低度相关性;在赛事密集期,运动员的Hb值低于“有氧运动亚理想值”,提示可能有过度疲劳的现象及运动性贫血的趋势。     

古典式青年摔跤运动员大强度训练期部分生化指标的监测研究

采用测试法,跟踪监测古典式青年摔跤运动员大强度训练期间部分生化指标。结果显示：血红蛋白在训练初期明显降低,是反映摔跤训练强度和运动员产生适应的敏感指标;训练期间的血尿素、血清肌酸激酶的“恢复值”可作为医务监督的重要参考指标;血清睾酮、血清皮质醇及其比值用作评价运动员机能状况。     

Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions

Introduction

The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation.

Materials and methods:

Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4ºC or 24ºC for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemical anaytes were measured on each sample. Variations remained under the desirable bias considered as clinically insignificant.

Results:

On day three, most analytes remained stable including albumin, protein, creatinine, cholesterol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Glucose concentration decreased markedly (P = 0.001) beginning from the first hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 ºC in gel tubes. Aspartate aminotransferase (AST) activity increased significantly (P = 0.002) up to 48-h, however bias was not significant clinically. High density lipoprotein (HDL) concentration was stable in gel tubes at 24 ºC, in plain tubes at 4 ºC stored up to 36-h.

Conclusion:

Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 ºC, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.     

血清肌酸激酶在游泳运动中的应用

本文通过查阅相关文献,阐述了肌酸激酶,分析了血清肌酸激酶与运动训练的关系,如何利用血清肌酸激酶对游泳运动进行监控以及在利用血清肌酸激酶监控时需要考虑的影响因素。     

学校体育对高校男生的肥胖干预研究

目的：基于校园体育俱乐部为载体,考虑学生的体育锻炼兴趣,探讨本运动干预方案对高校肥胖男生身体形态指标、血液生化指标的影响;方法：18～20岁肥胖大一男生共35名,按照BMI和个人意愿分为运动干预组（NE）和对照组（NC）,NE组进行有组织的90天、运动量递减（1～30天,大运动量;31～90天,小运动量）的运动干预。分别于运动前、30天后和90天后进行体格测试和空腹血脂、血糖和胰岛素水平检测。结果：1）NE组学生BMI、体脂率、腰围在经过30天和90天两个阶段综合干预后显著下降（P〈0.05）,NC组则前后变化不明显;2）NE组学生血清TG水平在30天后和90天后均显著下降（P〈0.05）,血清TC水平在90天后显著下降（P〈0.01）,LDL-C水平在90天后均显著下降（P〈0.01）,NE组HDL-C水平则无显著性变化（P〈0.05）;3）NE组学生空腹血糖和血清胰岛素水平在30后及90天后均出现显著下降（P〈0.01）,NC组则变化不明显。结论：基于校园俱乐部为载体的本运动干预模式可以有效降低学生的肥胖程度,改善其身体形态、血液生化指标,具有增进身体健康的作用。     

Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin

The binding of pefloxacin mesylate （PFLX） to bovine lactoferrin （BLf） and human serum albumin （HSA） in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Forster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.     

荧光光谱法研究间-硝基苯胺与牛血清白蛋白的相互作用

文章采用荧光光谱技术研究了在不同的酸度和温度条件下,间-硝基苯胺与牛血清白蛋白间的相互作用机制.采用荧光猝灭法讨论了不同pH条件下邻硝基苯胺与牛血清白蛋白间的猝灭类型,计算了不同温度和pH条件下的结合常数,并根据热力学参数确定了其主要结合作用力的类型.研究结果表明,间-硝基苯胺对牛血清白蛋白有较强的荧光猝灭作用,符合静态猝灭机理,二者之间的结合作用力主要是范德华力.     

地美硝唑与牛血清白蛋白结合热力学特征的荧光光谱研究

采用荧光光谱和紫外可见吸收光谱法研究了地美硝唑与牛血清蛋白的结合反应,发现地美硝唑对牛血清白蛋白有较强的荧光猝灭作用,该猝灭过程主要为静态猝灭过程。从荧光光谱数据,由Stern—Volmer方程和Lineweaver—Burk方程分析并处理实验数据得到了23℃时结合反应的结合常数K=1．03×10^4L／mol,结合位点数为0．70,结合反应的标准焓变、标准熵变、和标准吉布斯自由能变分别为～63．48kJ／mol,-96．22J／K,-35．00kJ／mol。