HDAC1、8在肺泡Ⅱ型上皮细胞间质转化中的表达及意义

目的：观察TGF-β1作用下,肺泡Ⅱ型上皮细胞RLE-6TN在EMT过程中HDAC1、8的表达情况及TSA对TGF-β1诱导细胞EMT的影响。方法：将TGF-β1加入到体外培养的细胞中,于不同时间收取细胞,采用WB及Real-time RT-PCR检测HDAC1、8及E-cad、α-SMA的表达情况。然后将TGF-β1加入经过TSA预处理的细胞中,于不同时间收取细胞,重新检测上述基因。结果：加入TGF-β1后,E-cad蛋白表达下调;α-SMA蛋白表达上调;HDAC1蛋白表达上调;HDAC8蛋白表达下调。E-cad mRNA表达下调;α-SMA及HDAC8的mRNA均于12 h表达上调,6 h、24 h表达下调;HDAC1 mRNA表达于6 h下调,24 h上调。加入TSA后,E-cad蛋白表达上调;α-SMA、HDAC1及HDAC8蛋白的表达均下调。E-cad mRNA表达上调;α-SMA mRNA表达于6 h、12 h上调,24 h下调;HDAC1 mRNA表达下调;HDAC8 mRNA表达上调。结论：TGF-β1体外诱导的RLE-6TN细胞EMT,可以通过应用TSA抑制其获得α-SMA表型、失去E-cad表型,从而部分逆转TGF-β1诱导的肺泡Ⅱ型上皮EMT。     

有氧运动对女性II型糖尿病患者HDL成熟的影响

目的：分析空腹和餐后,有氧运动对II糖尿病患者血浆脂蛋白特性、前B1一HDL浓度、体外HDL,清除巨噬细胞胆固醇和抑制LDL氧化能力的作用。方法：受试者分为三组,健康对照组（A,n=11）;II糖尿病运动组（B,n=11）;II糖尿病不运动组（C,n=11）。A和B组进行4个月的有氧运动。所有受试者都进行一次脂肪餐负荷试验以分析各种参数的基础值和最后值。结果：运动没有改变体重、BMI、血浆总胆固醇、LDL、HDL、TG、胰岛素浓度和HOMA—IR,但是降低了腰围。HDL3没有变化,有氧运动没有改变其清除细胞胆固醇的能力。糖尿病患者进行有氧运动能改善饥饿状态下HDL,阻止LDL最大氧化率能力的15％,降低餐后血浆前β1-HDL浓度,表明增加前β1-HDL向大的、更加成熟的HDL转化。就此而言,规律的有氧运动增加健康人空腹和餐后的HDL2和TG,增加糖尿病患者空腹时HDL2和TG。结论：研究结果显示糖尿病患者进行有氧运动改善HDL抑制LDL氧化的效力,帮助HDL成熟。这个结果不依赖于胰岛素抵抗的改变和血浆HDL浓度的变化。     

Modeling and multi-objective optimization of a gasoline engine using neural networks and evolutionary algorithms

In this paper, a multi-objective particle swarm optimization （MOPSO） algorithm and a nondominated sorting genetic algorithm II （NSGA-II） are used to optimize the operating parameters of a 1.6 L, spark ignition （SI） gasoline engine. The aim of this optimization is to reduce engine emissions in terms of carbon monoxide （CO）, hydrocarbons （HC）, and nitrogen oxides （NOx）, which are the causes of diverse environmental problems such as air pollution and global warming. Stationary engine tests were performed for data generation, covering 60 operating conditions. Artificial neural networks （ANNs） were used to predict exhaust emissions, whose inputs were from six engine operating parameters, and the outputs were three resulting exhaust emissions. The outputs of ANNs were used to evaluate objective functions within the optimization algorithms： NSGA-II and MOPSO. Then a decision-making process was conducted, using a fuzzy method to select a Pareto solution with which the best emission reductions can be achieved. The NSGA-II algorithm achieved reductions of at least 9.84%, 82.44%, and 13.78% for CO, HC, and NOx, respectively. With a MOPSO algorithm the reached reductions were at least 13.68%, 83.80%, and 7.67% for CO, HC, and NOx, respectively.     

Thermodynamics of Zn（II）-NaOH-H2O system

According to the principles of simultaneous equilibrium and electronic charge neutrality, the thermodynamics of Zn（II）-NaOH-H2O system was studied in this paper. Varying the concentration of sodium hydroxide, in range of 0- 400 g/L, the distribution law of all zinc species and the mathematical model of equilibrium concentration of zinc in the system were obtained. The relative errors between theoretic calculation and the experimental values of total concentration of zinc were analyzed, and the absolute average error is only 0.129 8%. The mathematical model and the thermodynamic data are believable.     

5-氨基-1，10-菲咯啉对羟基苯甲醛西佛碱铜配合物的合成、表征及与DNA作用研究

用过渡金属铜为配位中心,以5-氨基-1,10-菲咯啉对羟基苯甲醛西佛碱铜配合物为配体,合成了一种新的、可作为核酸荧光探针的金属配合物,并对其与DNA的作用作了初步的研究。     

Photosystem II-based biomimetic assembly for enhanced photosynthesis

Photosystem II (PSII) is a fascinating photosynthesis-involved enzyme, participating in sunlight-harvest, water splitting, oxygen release, and proton/electron generation and transfer. Scientists have been inspired to couple PSII with synthetic hierarchical structures via biomimetic assembly, facilitating attainment of natural photosynthesis processes, such as photocatalytic water splitting, electron transfer and ATP synthesis, in vivo. In the past decade, there has been significant progress in PSII-based biomimetic systems, such as artificial chloroplasts and photoelectrochemical cells. The biomimetic assembly approach helps PSII gather functions and properties from synthetic materials, resulting in a complex with partly natural and partly synthetic components. PSII-based biomimetic assembly offers opportunities to forward semi-biohybrid research and synchronously inspire optimization of artificial light-harvest micro/nanodevices. This review summarizes recent studies on how PSII combines with artificial structures via molecular assembly and highlights PSII-based semi-natural biosystems which arise from synthetic parts and natural components. Moreover, we discuss the challenges and remaining problems for PSII-based systems and the outlook for their development and applications. We believe this topic provides inspiration for rational designs to develop biomimetic PSII-based semi-natural devices and further reveal the secrets of energy conversion within natural photosynthesis from the molecular level.     

What are these among so many? Pre and post Vatican II recordkeeping

Contemporary Church recordkeeping has been an area all too often ignored by historians and archivists. There were two significant events in the last 140 years that defined the types of records created and maintained within the Church namely: Vatican I and Vatican II. After the suspension of Vatican I and the promulgation of Pastor Aeternus, we begin to see a centralization in ecclesiastical governance structures, due in part to interpretations of papal primacy. This centralization of Church authority eventually impinged upon the recordkeeping processes of dioceses. However after Vatican II the pendulum shifted with the promulgation of the Decree Concerning the Pastoral Office of the Bishop and the re-introduction of the tenet of collegiality. The promulgation of this document and others caused a de-centralization of Church recordkeeping practices. Using the Archdiocese of Vancouver as a case study this paper will attempt to show recordkeeping changes that occurred after Vatican I and Vatican II and the impact these councils had on archdiocesan administration.     

太极拳运动干预防治高血压病的实证研究

遵循运动处方原理和模式,以太极拳运动防治"肝肾阴虚证"高血压病为例,探讨其临床实效性,实验结果表明:1)16周实验后,实验组C-反应蛋白(CRP)浓度降低与实验前相比有差异。而对照组没有显著性变化。2)实验组收缩压较实验前有显著性降低,而舒张压有极其显著性降低。且实验前后收缩压、舒张压的差值与对照组相比,收缩压有显著差异,舒张压有极其显著性差异。3)运动后受试者感觉心情愉悦、平衡能力增强、头脑清醒、身体轻松、精力充沛、无不适感等情况,实验组为95.15%,对照组为76.08%,差异显著。受试者自觉失眠、多梦、头晕、耳鸣、乏力、烦燥等不适症状得到改善,其中实验组为90.33%,对照组为87.53%;实验组在医生指导下减少用药量,感觉轻松自如、精力充沛,自觉症状改善,血压呈下降和稳定状态,实验组无自觉症状者为41.68%,而对照组无自觉症状者为31.83%,有显著差异,表明太极拳运动处方防治高血压病效果显著,具有临床指导意义。     

岳麓秦简（贰）的分数研究价值

分数的发展史是汉语史的重要组成部分,分数研究方面论著亦颇多,然而学者们研究分数的材料多以传世文献为主,他们取得了很大的成绩,却也有很大的局限,主要问题是很难做到全面掌握材料,故论及多有缺陷。《岳麓书院秦简（贰）》作为中国最早的数学书,分数大量应用,对早期分数研究的价值不言而喻。     

网络课程的网页布局与导航样式对学习影响的眼动实验研究

Web网页是网络课程重要的人机界面,其布局和设计直接影响学习者的学习效果.本文从眼动心理学角度,利用眼动实验研究学习者利用网络课程学习的视觉搜索行为,测量学习者在3种常见的网页布局及其导航样式下学习时的眼动数据,探讨网页布局对网络学习的影响.通过记录注视次数、注视持续时间等6项眼动指标,并结合态度测量,试图揭示学习者学习时的眼动心理生理基本特征,以期找出适宜的网页布局形式和导航样式,为网络课程的设计和开发提出有效建议.实验研究表明：（1）T字型布局有利于学习者进行视觉搜索;（2）国字型布局更有利于学习者对知识的认知加工;（3）学习者进行视觉搜索时兴趣区集中在导航区,进行知识学习时兴趣区集中在主体内容区;（4）学习者在国字型布局下能取得更高的正确率.