中医药与运动性疲劳

针对中医对运动疲劳的认识、中医药在消除运动性疲劳中的作用及研究中存在的问题,如基础研究中存在的问题、建立运动性疲劳中医"证"的动物模型和实践中存在的不足,进行总结和梳理,旨在进一步加深中医对运动疲劳本质的认识,提高中医药抗疲劳针对性和效果,促进运动员大运动量训练后机体的恢复。     

心肌SarcKATP通道kir6.2亚基在运动预适应心肌保护效应中变化的研究

目的:探讨心肌SarcKATP通道kir6.2在运动预适应心肌保护效应中的变化以及与PKC的关系。方法:SD大鼠分对照组(C组)、力竭运动组(EE组)、运动预适应组(EP组)、运动预适应+力竭运动组(EP+EE组)、PKC阻断剂+运动预适应组(CHE+EP)和PKC阻断剂+运动预适应+力竭运动组(CHE+EP+EE组)。一次大强度间歇跑台运动建立运动预适应模型,力竭跑台运动致大鼠心肌损伤。用原位杂交和实时荧光定量PCR法检测kir6.2mRNA变化,用免疫荧光和免疫印迹法检测kir6.2蛋白变化。结果:与C组比,EE组和EP组kir6.2mRNA无明显变化,而EE组kir6.2蛋白明显升高,EP组kir6.2蛋白明显下降。与EE组比,EP+EE组kir6.2mRNA无明显变化,kir6.2蛋白明显下降。与EP组比,CHE+EP组kir6.2mRNA明显降低,kir6.2蛋白明显升高。与EP+EE组比,CHE+EP+EE组kir6.2mRNA和kir6.2蛋白无明显变化。结论:在EP诱导的保护效应中,心肌SarcKATP通道kir6.2mRNA水平未发生明显变化,而kir6.2蛋白水平明显降低,提示,心肌SarcKATP通道通过kir6.2蛋白水平的降低介导EP诱导的心肌保护效应。PKC对心肌SarcKATP通道的表达具有调控作用。     

基于ESI的中国农业大学植物与动物科学学科竞争力分析

基于ESI数据,以计量学理论为基础研究了进入ESI前1%(top1%)的中国农业大学植物与动物科学论文数、总引用量及其排名变化和高被引论文情况,并与国内外部分研究机构进行对比分析。从学科生产力、学科影响力、学科发展力、学科创新力四个方面对中国农业大学植物与动物科学的学科竞争力进行了定量分析与客观评价。结果表明,中国农业大学植物与动物科学的学科生产力、学科影响力水平较高,处于快速提升的阶段;学科发展力、学科创新力处于低水平,还需大幅提升。总体来看,中国农业大学植物与动物科学的学科竞争力已经初步形成并处于继续提升中,有望成为世界一流学科。     

注重学科差异 提高教学质量——以饲料加工工艺与设备为例

饲料加工工艺与设备课程具有鲜明的工科性质,为提高该课程在动科专业的教学质量,对学科结构、专业思维方式、课程教学以及理论学习与实践性教学之间的矛盾进行了分析,结合笔者实际教学经验,从四个方面提出了本课程在动科专业的教学思路,旨在提高教学质量。     

沈从文“湘西世界”中的“动物世界”

在沈从文精心构建的＂湘西世界＂中,存在着一个丰富多彩的＂动物世界＂,是＂湘西世界＂中不可或缺的重要组成部分。沈从文的＂动物世界＂具有烘托环境、渲染心境、推动叙事的多重功能,以动物朴实本真的生命之美映衬美好的人情人性,以动物鲜活生动的野性之美揭示城市文明的虚伪做作,构成了沈从文作品独特的艺术魅力。     

Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases

The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model for the study of human infectious diseases. In this study, we describe the development of a large-scale automatic multi-functional isolation chamber for use with medium-sized laboratory animals carrying infectious diseases. The isolation chamber, including the transfer chain, disinfection chain, negative air pressure isolation system, animal welfare system, and the automated system, is designed to meet all biological safety standards. To create an internal chamber environment that is completely isolated from the exterior, variable frequency drive blowers are used in the air-intake and air-exhaust system, precisely controlling the filtered air flow and providing an air-barrier protection. A double door transfer port is used to transfer material between the interior of the isolation chamber and the outside. A peracetic acid sterilizer and its associated pipeline allow for complete disinfection of the isolation chamber. All of the isolation chamber parameters can be automatically controlled by a programmable computerized menu, allowing for work with different animals in different-sized cages depending on the research project. The large-scale multi-functional isolation chamber provides a useful and safe system for working with infectious medium-sized laboratory animals in high-level bio-safety laboratories.     

实验用大白鼠蛲虫感染状况调查

目的：了解某高校实验动物大自鼠蛲虫感染情况,为医学实验提供符合标准的实验动物。方法：按照国家标准《实验动物寄生虫学》检测方法,用粪便生理盐水涂片法检查。结果：共检测1月龄、2月龄大白鼠69只,其中感染蛲虫鼠17只,阳性率25％。结论：为确保实验动物质量,应定期对动物进行寄生虫学检测,以保障实验人员安全,保证实验的可信度、严谨性。     

Comparison of Haptic and Biometric Properties,Bacterial Load,and Student Perception of Fixative Solutions: Formaldehyde Versus Chilean Conservative Fixative Solution with and without Formaldehyde in Pig Kidneys

One of the most widely used solutions to fix and preserve organic tissues is formaldehyde, despite reservations regarding its toxicity and the fact that formaldehyde-embalmed bodies lose their original characteristics. Anatomy laboratories have been replacing formaldehyde with solutions that retain the characteristics of fresh tissue. For this purpose, alternative solutions with a very low concentration of formaldehyde or without any formaldehyde have been analyzed. The objective of this study was to compare biometry, coloration, haptic properties, and bacterial load on animal specimens (pig kidneys) embalmed with formaldehyde, and with Chilean Conservative Fixative Solution with and without formaldehyde (formaldehyde chCFS and formaldehyde-free chCFS). Also, the perception of health and biological science students toward specimens treated with different solutions was assessed. The results indicated that there were no significant differences in specimens’ retraction, or bacterial load. Students showed a preference for organs embalmed in formaldehyde chCFS and formaldehyde-free chCFS; indicating that with these treatments they could better visualize structures and that the prosections had greater flexibility and the colors were more similar to those of fresh tissue. Additionally, students recommended the material embalmed in formaldehyde chCFS and formaldehyde-free chCFS for anatomy learning. In contrast, students indicated that formaldehyde-fixation negatively affected their practical experience. In conclusion, embalming with formaldehyde chCFS or formaldehyde-free chCFS provides an advantageous practical experience over the use of formaldehyde and may be an alternative to replace the use of formaldehyde in anatomy laboratories.     

基于主题相关分析的跨学科主题发现方法及实证研究——以动物资源与育种领域为例

[目的/意义] 针对单学科和双学科主题发现方法无法挖掘现有交叉文献中主题演化来源的问题,提出面向跨学科的主题发现方法,为跨学科发展和合作提供依据。[方法/过程] 首先在动物资源与育种领域期刊文献数据中选取已经出现交叉现象的两个基础学科文献及其交叉文献,使用改进的主题相关分析方法,提取共同主题和各自的独立主题;然后利用相关性测度方法量化不同学科独立主题的相关性;最后对共同主题和相似性较高的独立主题进行具体分析。[结果/结论] 在动物资源与育种领域的农学生殖生物学、兽医学以及其交叉文献上进行实验验证,结果表明所提出的方法能够有效发现交叉主题的学科出处。     

英汉俗谚中的隐喻比较及其差异根源

隐喻是我们赖以生存的一种语言现象和认知模式,它受社会文化背景影响,表现出明显的文化特征.从英汉隐喻的来源人手,通过对英汉一些常见的动、植物词汇和人体词汇隐喻的实例分析及其差异根源的探究,可以揭示英汉隐喻现象所折射出来的不同民族文化心理及其对跨文化交际的影响.