Antioxidant activity ofcurculigo orchioides in carbon tetrachloride—induced hepatopathy in rats

In this study antioxidant activity of methanol extract of rhizomes ofCurculigo orchioides (MEC) was investigated using carbon tetrachloride (CCl₄)-intoxicated rat liver as the experimental model. The hepatotoxic rats were administered MEC for 90 days (daily, orally at the dose of 70 mg per kg body weight). Lipid peroxidation (LPO) in CCl₄-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and diene conjugates (CD), and also a distinct diminution in glutathione (GSH) content in the liver. In CCl₄+MEC—treated rats these biochemical parameters attained an almost normal level. The decreased activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GRD) in CCl₄—intoxicated rats, and its retrieval towards near normalcy in CCl₄+MEC—administered rats revealed the efficacy of MEC in combating oxidative stress due to hepatic damage. Elevated level of glutathione transferase(GTS) observed in hepatotoxic rats too showed signs of retuming towards normalcy in MEC co-administered animals, thus corroborating the antioxidant efficacy of MEC. The findings provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.     

新态势下我国外汇储备管理转型的路径研究

屡创新高的政府外汇储备与徘徊不前的民间外汇储备是当前我国外汇储备的两大显著特征。由于传统的外汇储备管理在认知上存在着偏差,管理对象仅限于政府储备,忽略了潜力巨大的民间储备,再加上其颇具机械性的运营模式,进而放大了储备管理的风险。基于此,可从"源头"与"源尾"两方面着手对外汇储备管理实施转型:对"源头"——强制结售汇制进行改革,充分落实由"藏汇于国"到"藏汇于民"的战略转变;对"源尾"——巨额储备资产运作方式加以革新,对超适度外汇储备进行积极管理,大力实施能力储备战略。     

Influence of fasting on muscle composition and antioxidant defenses of market-size Sparus macrocephalus

The study was conducted to investigate fasting effects on flesh composition and antioxidant defenses of market-size Sparus macrocephalus. Two hundred fish (main initial weight 580 g) were divided into two groups (control and fasted) and reared in 6 cages. After two weeks of adaptation, group I fasted for 28 d; group II was fed normally as a control. In 3, 7, 14, 21 and 28 d, 6 fish per group were sampled for proximate flesh composition, liver antioxidant enzyme activities and malondialdehyde flesh content analyses. In fasted fish, the reduction of lipid content in muscle occurred after day 3, and, compared to controls, the content of protein decreased from day 14, the activities of liver antioxidative enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) increased from day 3, and flesh malondialdehyde levels increased from day 21. Flesh fat reduction shows that fasting may be used as a technique to reduce flesh lipid content in Sparus macrocephalus. However, considering flesh protein loss and the subsequent oxidative stress, the fasting technique should be used with precautions. Project (No. 2006C12098) supported by the Science and Technology Department of Zhejiang Province, China     

Factors Affecting Performance of Agricultural Extension: Evidence from Democratic Republic of Congo

Purpose: As part of the institutional reforms and agricultural restructuring in the Democratic Republic of Congo (DRC), this paper provides an assessment of the performance of the agricultural extension system as well as factors explaining it.

Method: This paper involves key informants’ interviews and surveys of 107 extension organizations and 162 extension agents in randomly selected 156 villages, analyzed using qualitative and logistic regression methods.

Findings and Practical Implications: Results show that despite having one of the highest extension agent-to-farmer ratio and a pluralistic extension system, DRC fails to deliver knowledge and technologies to rural areas due to lack of coordination, no unified and clear policy and mandate, lack of funding, aging and low competencies of agents, and lack of mobility and interactions of agents with key actors. This paper complements findings by other studies that number of agents is not a sufficient indication of performance, but an effective system needs to focus on the enabling environment for agents to be motivated to work as mandated. In this paper, enabling conditions that are found to be statistically significant are external funding, enforcement of performance targets, systems of rewards and sanctions, mobility to foster linkages, and skills development.

Originality: This paper contributes by: (1) analyzing a cross-section of various organizations and agents to identify factors that explain variations in performance in a statistical and systematic approach; (2) providing insights on how to prioritize investments and options for a fragile state like DRC, with weak infrastructure and institutional capacity and with a long history of neglect for their national extension system; and (3) illustrating how a rich and well-cited conceptual framework can be implemented empirically to provide policy options for a country like DRC.     

米糠抗氧化肽的分离纯化研究

以DEAE-52离子交换柱层析和SephadexG-25凝胶过滤柱层析纯化米糠抗氧化肽,以硅胶G薄层层析测定其纯度,氨基酸自动分析仪测定其氨基酸组成。实验结果表明:米糠抗氧化肽经DEAE-52柱层析和Sephadex G-25柱层析后基本上得到了纯化;氨基酸组成分析表明,米糠抗氧化肽由14种氨基酸组成,富含两种酸性氨基酸:Asp、Glu,并含有6种人体必需氨基酸(Val、Met、Ile、Leu、Phe、Lys),占米糠抗氧化肽氨基酸总含量的31.89%,具有较高的营养价值和保健功能。     

Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties

The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx),and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2 g/kg). On the contrary, increased activities of liver GSH, SOD, GPx,CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepatoprotective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.     

教育机构侵权责任实证研究

分析2018年全国以限制民事行为能力人为受害人的116起教育机构侵权案件发现，教育机构承担责任的判决占93%。样本案例反映出法院采用的归责原则、举证责任分配规则与法律规定不尽相符，对教育管理职责的认定宽泛,要求教育机构及时发现伤害但不具有现实可行性，认可禁止跑闹的校规但此类规定不尽合理等问题。建议剥离意外事故，采用举证责任倒置规则，以专业教育工作者作为教育管理职责的判断标准，培育由受教育者自担部分责任的文化。     

鸡传染性贫血病弱毒苗免疫对肝线粒体抗氧化酶活性的影响

观察鸡传染性贫血病弱毒苗多次免疫对肝线粒体中抗氧化酶活性的影响。方法：20只SPF鸡随机分为2组,每组10只,免疫组鸡用鸡传染性贫血弱毒苗免疫四次,每次间隔2W,对照组鸡用同剂量的生理盐水。最后一次免疫后10d取肝脏制备线粒体,利用测试盒测定肝线粒体中的GSH-PX活性、SOD活性和MDA含量。结果：对照组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为20．444±2．64U／mgprot、15．59±1．33U／mgprot和7．32＋1．89nmol／mL,免疫组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为24．85±3．53U／mgprot、24．284±4．17U／mgprot和5．264±1．24nmol／mL。与对照组相比,免疫组肝线粒体中GSH-PX活性显著提高（P〈0．05）,SOD活性极显著提高（P〈0．01）,MDA含量显著减低（P〈0．05）。结论：鸡传染性贫血病多次免疫可提高机体抗氧化能力。     

中国研究型大学创业能力研究——基于多元统计分析

作者运用多元统计方法对2004-2008年“985工程”高校的创业能力进行了研究。结果表明创业能力包括专利成果、产业绩效和技术转移三个因素,其中专利成果是“985工程”高校最重要的创业能力;专利成果、技术转移和产业绩效与来自政府的科研经费显著相关;对“985工程”高校创业能力相关数据进行的Q型聚类分析表明,四种类型大学创业能力之间的差异非常大。     

浅谈不同进制数之间的转换方法与技巧

计算机文化基础与应用是大中专院校必学的基础课程,其中不同进制数之间的转换不仅是课堂教学的重点内容之一,也是国家计算机一级B必考的内容。本文通过对不同进制数之间的转换方法进行归纳与比较,帮助大家搞清楚它们之间的区别,在提高学习兴趣的同时,帮助同学们掌握不同进制数之间的转换的基本方法和技巧。