Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro

Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies.     

Determination of thigh volume in youth with anthropometry and DXA: Agreement between estimates

Abstract

This study examined the agreement between estimates of thigh volume (TV) with anthropometry and dual-energy x-ray absorptiometry (DXA) in healthy school children. Participants (n=168, 83 boys and 85 girls) were school children 10.0–13.9 years of age. In addition to body mass, height and sitting height, anthropometric dimensions included those needed to estimate TV using the equation of Jones & Pearson. Total TV was also estimated with DXA. Agreement between protocols was examined using linear least products regression (Deming regressions). Stepwise regression of log-transformed variables identified variables that best predicted TV estimated by DXA. The regression models were then internally validated using the predicted residual sum of squares method. Correlation between estimates of TV was 0.846 (95%CI: 0.796–0.884, S_y·x=0.152L). It was possible to obtain an anthropometry-based model to improve the prediction of TVs in youth. The total volume by DXA was best predicted by adding body mass and sum of skinfolds to volume estimated with the equation of Jones & Pearson (R=0.972; 95%CI: 0.962–0.979; R ²=0.945).     

The effect of training on aerobic power characteristics of young cross‐country skiers

The influences of growth, training and various training methods were investigated by analysing long‐term training effects in young cross‐country and biathlon skiers (n = 129). Some athletes (n = 49) were studied six times in three years and some at least once a year during a four year period (n = 48). During three summer training periods skiers emphasized either intensive training or distance training or continued to train normally. The results indicated that maximal oxygen uptake (VO₂ max) and heart volume increased between 15 and 20 years of age and the most significant changes in heart volume were observed between 16 and 18 years of age. International level skiers were able to increase their VO₂ max and heart volume even after 20 years of age. Anaerobic threshold (AT, ml kg^‐1 min^‐1) increased like VO₂ max but when expressed as a percentage of VO₂ max, the AT was similar in every age group over 16 years of age. Intensive training at the intensity of anaerobic threshold or higher was observed to be most effective in producing improvements in VO₂ max. Low‐intensity distance training was more effective in producing improvements in anaerobic threshold.     

Recovery of pulmonary diffusing capacity after maximal exercise

Abstract

Pulmonary diffusing capacity (Dl_co), together with spirometric variables, arterial oxygen tension (paO₂) and cardiac output were determined before and at intervals after maximal arm cranking, treadmill running and erogmeter rowing. Independent of the type of exercise, Dl_co increased immediately post‐exercise from a median 13.6 (range 7.3–16.3) to 15.1 (9.3–19.6) mmol min^‐1 kPa^‐1 (P <0.01). However, it decreased to 11.6 (6.9–15.5) mmol min^‐1 kPa^‐1 (P <0.01) after 24 h with cardiac output and paO₂ at resting values, and Dl_co normalized after 20 h. Thoracic electrical impedance at 2.5 and 100 kHz increased slightly post‐exercise, indicating a decrease in thoracic fluid balance, and there were no echocardiographic signs of left ventricular failure at the time of the decrease in Dl_co. Also, active muscle (limb) circumference and volume, and an increase in haematocrit from 43.8 (38.0–47.0) to 47.1 (42.7–49.8) (P <0.01), had normalized at the time of the decrease in Dl_co. Vital capacity, forced vital capacity, forced expiratory volume in 1 s, peak and peak mid‐expiratory flows did not change. However, total lung capacity increased from 6.8 (5.0–7.6) to 7.0 (5.1–7.8) litres (P <0.05) immediately after exercise and remained elevated at 6.9 (5.1–8.7) litres (P <0.05) when a decrease in Dl_co was noted. The results demonstrate that independent of the type of maximal exercise, an approximate 15% reduction in Dl_co takes place 2–3 h post‐exercise, which normalizes during the following day of recovery.     

Comparison of Hydrostatic Weighing at Residual Volume and Total Lung Capacity in Children

Abstract

The influence of perceptions of the motivational climate and perceived ability on situational motivation and the physical activity behavior of 213 male and 229 female adolescent physical education students (M age= 12.56 years; SD = 0.96) was examined over a 3-day period. A significant age by gender interaction emerged, with physical activity declining from the sixth to eighth grade. The decline was more pronounced among female than male students. Perceptions of a mastery climate were strongly related to more self-determined forms of situational motivation. In contrast, perceptions of a performance climate were strongly related to less self-determined forms of situational motivation. Results of a hierarchical regression analysis revealed gender, perceived ability, and perceptions of a mastery climate to explain a significant amount of variance in physical activity. These findings suggest that promoting a mastery oriented motivational climate in physical education will foster self-determined situational motivation and physical activity.     

基于ARIMA模型的体育彩票销量预测——以上海为例

对未来体彩销量预测分析,可以为体彩产品设计、营销推广提供科学依据。本文以上海为例,采用ARIMA模型对2009年1月-2013年3月的上海体育彩票的月度销售数据进行时间序列分析,并预测出2013-2015三年上海体育彩票的销量,从而对上海体育彩票的销量增长趋势进行分析。     

新工科背景下流体力学教学方法研究与实践

针对新工科建设背景和需求,分析流体力学在建筑环境与能源应用工程专业中的地位,探索流体力学的教学新模式。在课程设置上进行学科交叉、融合;在理论教学过程中,结合专业综合实验台进行讲述;课程实验打破学科之间的壁垒,锻炼学生的动手能力,拓宽学生的知识领域,增强学生的专业技能。     

Investigation of blood pulse PPG signal regulation on toe effect of body posture and lower limb height

Objective: To study the regulation of blood pulse volume via photoplethysmography (PPG) signal detected from toe, while the lower limb is passively raised in different height positions. Methods: Use a modified non-invasive PPG technique to detect the blood pulse signal on toe with infrared (IR) photo sensor. A protocol consisting of two postures, i.e., supine and 45° reclining, was designed to conduct laboratory trial in this study. During the period of performing the protocol of these postures, the lower limb was passively raised from the heights of 10 cm to 60 cm randomly and individually with sponge blocks underneath the foot. Results: In the supine posture, the higher the foot was passively raised, the more the blood PPG signal decreased. In the 45° reclining posture, the blood PPG signal increased at the beginning and then decreased in the foot height position from 10 cm to 60 cm. In both postures the normalized AC signal changes significantly while the normalized DC signal changes little. Conclusion: The toe PPG signals can obviously indicate the regulated blood volume change with the designated postural procedures due to the heart level position.     

关于旋转体体积的研究

阐述了平面图形绕坐标轴旋转产生的旋转体体积与形心坐标之间的联系。在此基础上把它推广到绕任意直线旋转的情况。并得出求旋转体体积的一般，公式。     

岩巷瓦斯异常涌出及防治

岩巷瓦斯异常涌出是近年来矿井瓦斯涌出时常发生的一种新形式,涌出时间短,瓦斯量大,有的伴有动力现象,目前还没有规律性,治理难度较大;结合淮南矿区瓦斯异常涌出事故的实例,探讨了岩巷瓦斯异常涌出的原因,提出了相应的防治措施,对消除安全生产威胁,促进开拓进度提高,具有一定的意义.