建国后我国基础教育体育课程内容研究的回顾与展望

采用文献资料法、逻辑分析等方法,对建国后中国基础教育体育课程内容研究的现状进行梳理与分析。结果表明,建国后中国基础教育体育课程内容研究主要集中在体育课程内容选择、组织、分类、开发利用的研究以及体育课程内容改革的研究等方面,存在研究深度与广度不足、实证研究相对薄弱、缺乏对体育课程内容整体性设计的研究等局限,在今后的研究中需要加强对体育与健康课程内容标准的研究、基础体育课程内容体系的整体建构研究,挖掘研究视角、创新研究思路,丰富研究方法,增强研究的应用性。     

基于知识图谱的我国体育课程改革研究综述

从中国知网（CNKI）数据库搜索到1990—2013年间发表的1643篇体育课程改革研究论文,运用共现分析、聚类分析等方法,借助信息可视化软件CitespacelI,绘制出我国体育课程改革研究的代表人物与机构分布、研究热点的知识图谱,从而直观展示当前体育课程改革研究的现状及发展趋势,为今后体育课程改革研究提供参考。     

对高校体育教师观念更新和角色转变的研究

从总结教学改革中存在的问题入手，站在学校体育思想发展的历史高度，分析新时期体育教师观念变更和角色的塑造，旨在建立体育教师新的教育思想，为高等教育改革提供指导。     

水稻P1B型ATPase重金属转运蛋白的结构与功能研究进展

P1B型ATPase重金属转运蛋白（HMA）广泛存在于低等植物和高等植物中,参与植物生长发育过程中所必需的微量金属营养元素的运输.与双子叶植物拟南芥（Arabidopsis thaliana）AtHMA1-8的大量研究相比,单子叶植物中HMA转运蛋白的相关功能与研究极其有限.根据已有的研究成果对水稻（Oryza sativa L.）体内9种HMA转运蛋白的分类、结构、分子机制与转运功能进行了详细对比和归纳,对这些重金属转运蛋白在植物中的组织分布、亚细胞定位和金属特异性进行了阐述,推测了目前尚未明确功能的OsHMA1、OsHMA4、OsH-MA6、OsHMA7和OsHMA8的亚细胞定位及潜在的转运功能,以期为未来进一步的科学研究提供更多依据.     

高职院校内涵发展战略:资源整合

资源整合是促进高职院校内涵发展的有效手段和途径,它具有聚焦、聚强、聚变、聚效功能。在高职院校相对投入不足,整体办学条件亟待改善,资源配置存在不合理状况的情况下,加强资源整合具有重要的现实意义和迫切性。资源整合应坚持有利于促进人才培养质量和特色兴校,有利于充分利用资源、实现增值、整体功能发挥最大化及可持续发展的原则。资源包括有形和无形资源,整合应突出重点,统筹兼顾。资源整合必须采取更新观念,做好自我诊断、创新整合方法,改革管理体制,建立健全长效机制等方法。     

P2P技术浅析

文章先将P2P模式与传统的C/S模式相比较,并论述了P2P技术应用的几个领域、技术分类及其存在的一些问题.     

作物淀粉黏度单位的几种表示方法浅议

作物淀粉黏滞性(黏度性状)值是淀粉的一项重要品质指标,然而其单位表示方法至今仍不为广大科研工作者和科技期刊编辑所熟悉.本文总结和探寻了目前作物淀粉黏度单位在国内外科技期刊中几种表示方法及其来源,阐述了这几种表示方法之间的相互关系,并剖析了某些错误的表示方法.     

Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro

BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006     

BitTorrent下载技术研究

BitTorrent是第四代网络下载技术,是一种多点下载、源码公开的p2p软件,通过构建覆盖网实现协作并发下载.BitTorrent协议是一种公开的P2P协议,分为节点问协议和节点与跟踪器协议.     

Openness and innovation—Home and export demand effects on manufacturing innovation: Panel data evidence for Ireland and Switzerland

Recent studies in the tradition of Schmookler have re-emphasised the potential role of demand in stimulating innovation. Here, we reconsider the role of ‘home’ and ‘export’ market demand in stimulating manufacturing innovation using comparable panel data for two small open economies - Ireland and Switzerland. Our analysis is based on the estimation of reduced form innovation production functions using panel data estimators over the sample period 1994-2005. For a range of innovation indicators, however, we find little evidence of any significant market demand effects, with innovation performance instead determined largely by firm-level capability effects and characteristics. In policy and strategy terms this suggests the continued value of measures to improve innovation capability regardless of market demand conditions. In more methodological terms our results suggest the validity of the usual assumption implicit in modelling innovation outputs that supply-side factors predominate.