Effect of short term vitamin E supplementation on lipid peroxidation in acute myocardial infarction

In view of sufficient body of evidence to substantiate the role of free radical mediated oxidative injury in myocardial infarction (M.I.), the utility of exogenous supplementation with antioxidant substance, vitamin E was studied. Forty patients of acute myocardial infarction were divided into two equal groups, one who received vitamin E and the other who did not. Plasma MDA levels showed a reduction in patients of M.I. who received vitamin E as well as in those who did not, however, the decrease was significantly more in the vitamin E treated subgroup.     

Antioxidant activity ofcurculigo orchioides in carbon tetrachloride—induced hepatopathy in rats

In this study antioxidant activity of methanol extract of rhizomes ofCurculigo orchioides (MEC) was investigated using carbon tetrachloride (CCl₄)-intoxicated rat liver as the experimental model. The hepatotoxic rats were administered MEC for 90 days (daily, orally at the dose of 70 mg per kg body weight). Lipid peroxidation (LPO) in CCl₄-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and diene conjugates (CD), and also a distinct diminution in glutathione (GSH) content in the liver. In CCl₄+MEC—treated rats these biochemical parameters attained an almost normal level. The decreased activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GRD) in CCl₄—intoxicated rats, and its retrieval towards near normalcy in CCl₄+MEC—administered rats revealed the efficacy of MEC in combating oxidative stress due to hepatic damage. Elevated level of glutathione transferase(GTS) observed in hepatotoxic rats too showed signs of retuming towards normalcy in MEC co-administered animals, thus corroborating the antioxidant efficacy of MEC. The findings provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.     

迷迭香对运动大鼠心肌组织脂质过氧化损伤保护作用的研究

选用SD大鼠，递增强度训练建立运动模型，研究迷迭香对运动大鼠心肌脂质过氧化损伤的保护作用。结果显示：迷迭香可显著降低血清谷氨酸草酰乙酸转氨酶（sGOT）的活性（P〈0．05），在心肌组织中谷氨酸草酰乙酸转氨酶的活性液呈现下降趋势；迷迭香可以不同程度地增强心肌组织中抗氧化酶SOD、CAT和GSH-Px的活性，均有显著性差异（P〈0．05）；迷迭香可以降低心肌组织中MDA的含量，有显著性差异（P〈0．05）。结论：迷迭香可以增强心肌组织中的抗氧化酶活性，减轻大强度耐力训练对大鼠心肌组织造成的脂质过氧化损伤。     

不同强度运动对高脂饮食大鼠脂代谢影响

目的:探讨不同强度运动对高脂饮食大鼠脂代谢的影响。方法:8周龄雄性SD大鼠32只随机分为对照组(C组)、高脂喂养组(HF组)、有氧运动高脂喂养组(E组)、大强度运动高脂喂养组(HE组),每组8只。E组大鼠采用无负重游泳训练,HE组大鼠采取5%体重负重游泳训练,共8周,60min/d,6d/w。训练末次称重并取大鼠血液进行血脂4项及全身脂肪含量测定。结果:与C组相比,HF组大鼠体重及全身脂肪含量明显增加,血TC、TG、LDL-C明显升高,HDL-C明显降低,并具有显著性差异(P<0.05)。E组、HE组体重、血脂4项、全身脂肪含量与C组比无明显差异。HE组全身脂肪及血脂4项高于E组。结论:有氧运动更有利于预防高脂饮食引起的脂代谢紊乱。     

Effect of Oxidized Phosphatidylcholine on Biomarkers of Oxidative Stress in Rats

In this study it was planned to investigate the effect of oxidized phosphatidylcholine (derived from egg) feeding on lipid peroxidation of different tissues in rats. Male Wistar albino rats were fed oxidized and unoxidized phosphatidylcholine for 2 and 4 weeks, respectively. During the period of study food intake and body weights of animals increased gradually. Animals fed oxidized phosphatidylcholine for 2 and 4 weeks showed 33 and 15% spontaneous hemolysis of red blood cells in vitro. Under identical experimental conditions animals given unoxidized phosphatidylcholine showed 14.5 and 13.4% hemolysis for 2 and 4 week’s period, respectively. Thiobarbituric acid reactive substances (TBARS) level in thymus, spleen, kidney, heart, liver and lung significantly increased in rats given oxidized phosphatidylcholine as compared to unoxidized group. Furthermore, in oxidized phosphatidylcholine group TBARS values in kidney, liver and lungs continued to rise for 4 weeks of treatment while TBARS level in heart, spleen and thymus was found to be decreased at the end of 4 weeks of oxidized phosphatidylcholine feeding. Plasma triacylglycerol and cholesterol was found to increase in rats who had received oxidized phosphatidylcholine for 2 weeks. These findings suggest that excess and persistent intake of oxidized phosphatidylcholine can cause significant damage to organs.     

运动对脂质代谢中ATGL的影响研究

脂解过程是对储存在细胞中脂滴的一个复杂的协调代谢过程,脂肪甘油三酯脂肪酶（ATGL）是催化甘油三酯水解的重要脂肪酶,在脂肪代谢中发挥着重要作用。通过文献综述,对脂滴的生物学功能、ATGL缺乏产生的影响、脂解和ATGL调节,以及运动训练对ATGL的影响等研究进展进行了梳理。指出,ATGL在脂解过程中起着关键作用,运动训练尤其是耐力运动可促进ATGL在机体内表达的增加,促进脂质代谢。     

Lipid profile in middle class Bengali population of Kolkata

Fasting samples of 1396 apparently healthy, middle class Bengali population of Kolkata, West Bengal were tested for total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol and very low density lipoprotein cholesterol, over a period of three years. The values obtained were (in mg/dl) 190±33, 132±42, 53±10, 116±30 and 21±7 respectively. When these subjects were grouped according to the age and sex, no appreciable difference were observed between most of the groups. Triglyceride was found to be low and HDL cholesterol was high in women below 30 years when compared with men of similar age. Beyond 60 years, cholesterol level as well as low density lipoprotein cholesterol was found to be gradually increased in case of women. Besides these changes, other minor differences were not statistically significant. It is suggested that the lipid values of the present study should be taken as a base parameters and the clinical evaluation be made on the basis of these finding.     

Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings

Objective:To study the oxidative stress and antioxidative response of Cinnamomum camphora seedlings exposed to nitrogen dioxide (NO2) fumigation.Methods:Measurements were made up of the growth,chlorophyll content,chlorophyll fluorescence,antioxidant system and lipid peroxidation of one-year-old C.camphora seedlings exposed to NO2 (0.1,0.5,and 4 μl/L) fumigation in open top chambers over a period of 60 d.Results:After the first 30 d,0.5 and 4.0 μl/L NO2 showed insignificant effects on the growth of C.camphora seedlings.However,exposure to 0.5 and 4.0 μl/L NO2 for 15 d significantly reduced their chlorophyll content (P0.05),enhanced their malondialdehyde (MDA) content and superoxide dismutase (SOD) activity (P0.05),and also significantly reduced the maximal quantum yield of PSII in the dark [the ratio of variable fluorescence to maximal fluorescence (Fv/Fm)] (P0.05).In the latter 30 d,0.5 μl/L NO2 showed a positive effect on the vitality of the seedlings,which was reflected by a recovery in the ratio of Fv/Fm and chlorophyll content,and obviously enhanced growth,SOD activity,ascorbate (AsA) content and glutathione reductase (GR) activity (P0.05);4.0 μl/L NO2 then showed a negative effect,indicated by significant reductions in chlorophyll content and the ratio of Fv/Fm,and inhibited growth (P0.05).Conclusion:The results suggest adaptation of C.camphora seedlings to 60-d exposure to 0.1 and 0.5 μl/L NO2,but not to 60-d exposure to 4.0 μl/L NO2.C.camphora seedlings may protect themselves from injury by strengthening their antioxidant system in response to NO2-induced oxidative stress.     

杂交水稻叶片衰老与膜脂过氧化作用的关系

杂交水稻汕优63主茎在抽穗后10d，其叶片自上而下表现出叶绿素和蛋白质含量下降．光合作用速率降低．丙二醇和脂质红过氧化物含量明显增加．SOD活性则为倒三叶＞倒四叶＞倒二叶＞旗叶．实验结果表明：叶片自下而上顺序衰老．叶片衰老与膜脂过氧化作用密切相关．     

维生素E和硒对运动中红细胞氧化应激的影响

给大鼠腹腔内注射维生素E亚硒酸钠注射液共45天,测定红细胞抗氧化酶、膜脂质过氧化物、膜流动性、膜蛋白高分子聚合物,以期了解疲劳性运动,氧化应激和抗氧化防御体系之间的关系。研究发现VitE和Se协同作用,有效地提高了大鼠红细胞SOD,GSH-Px活性,抑制疲劳性游泳后红细胞膜MDA含量增加。