力竭游泳对大鼠肠组织MDA、游离巯基和ATP含量的影响

探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30 min组(EX30);运动后60 min组(EX60),测定力竭性游泳后不同时相,肠组织匀浆MDA、游离巯基(Free-SH)和ATP含量。结果显示,运动后肠组织MDA含量在运动后30 min,60 min显著性增加(P0.01);运动后Free-SH含量在运动后30min(P0.05)和运动后60 min后(P0.01)显著下降;运动后30 min组ATP含量显著下降(P0.01)。结果提示,运动源性自由基产生增加,使得肠组织中游离巯基被氧化,导致ATP含量下降,可能是造成运动性肠功能紊乱的重要因素之一。     

Influence of pranayamas and yoga-asanas on serum insulin,blood glucose and lipid profile in type 2 diabetes

A distinguishable feature of type 2 diabetes besides hyperglycemia and deranged lipid profile is an impaired insulin secretion, peripheral insulin resistance and obesity which has become a major health concern worldwide. India with an estimated 31million diabetics in 2000 and 79mllions by the yr 2030 has the highest number of type 2 diabetics in the world. In this study, we aimed to see if yoga-asanas and pranayamas have any influence in modifying certain biochemical parameters. Sixty patients of uncomplicated type 2 diabetes (age 35–60 yrs of 1–10 yrs duration) were divided into two groups: Group 1 (n=30): performed yoga along with the conventional hypoglycemic medicines and group 2 (n=30): patients who only received conventional medicines. Duration of the study was 45 days. Basal recordings of blood glucose (fasting and post-prandial), lipid profile and serum insulin were taken at the time of recruitment and the second reading after forty five days. Results showed a significant improvement in all the biochemical parameters in group 1 while group 2 showed significant improvement in only few parameters, thus suggesting a beneficial effect of yoga regimen on these parameters in diabetic patients.     

Protective influence of vitamin E on the antioxidant defence system in the whole blood and liver of normal and alloxan-induced diabetic rats

The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels, antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while the level of lipid peroxidation decreased.     

RBC membrane composition in insulin dependent diabetes mellitus in context of oxidative stress

Glyco-oxidation is considered as a source of permanent, cumulative, oxidative damage to long lived proteins in ageing and in diabetes. Although RBC depends solely on glucose for energy purpose, hyperglycemic state glycosylates hemoglobin, creates oxidative stress and puts the cellular components at risk. In view of this, RBC membrane composition was analyzed in diabetic patients. The results were compared with healthy age and sex matched control groups. When RBC membrane components such as protein, sialic acid, phospholipids and cholesterol were determined in insulin dependent diabetes mellitus, a significant rise in phospholipids and cholesterol and significant fall in sialic acid and protein content was noted. RBC membrane composition showed pronounced alterations in insulin dependent diabetes mellitus. These changes were accompanied by higher levels of lipid peroxidation products like Malondialdehyde.     

Effect of short term vitamin E supplementation on lipid peroxidation in acute myocardial infarction

In view of sufficient body of evidence to substantiate the role of free radical mediated oxidative injury in myocardial infarction (M.I.), the utility of exogenous supplementation with antioxidant substance, vitamin E was studied. Forty patients of acute myocardial infarction were divided into two equal groups, one who received vitamin E and the other who did not. Plasma MDA levels showed a reduction in patients of M.I. who received vitamin E as well as in those who did not, however, the decrease was significantly more in the vitamin E treated subgroup.     

A study of spectrin and lipid peroxidation of red blood cell membrane in thalassaemia carrier

The aim of the present work is to understand the lipid peroxidation of RBC membrane and the spectrin protein content of RBC membrane cytoskeleton of thalassaemic carrier state (trait) of β and hemoglobin E variant (HbE). We have measured the hemoglobin (Hb), malondialdehyde (MDA) and spectrin content of RBC membrane of thalassaemic carrier. The spectrin content (α and β band) of both β and HbE carrier was not changed than normal individuals. However, lipid peroxidation of RBC membrane was significantly increased in both β and HbE trait, and Hb level was also decreased in thalassaemic carrier. It may be assumed that oxidative damage by excess lipid peroxidation may have no role on irreversible membrane damage in β thalassaemia and HbE thalassaemia carrier.     

运动对大鼠心肌线粒体游离钙的影响

采用游泳运动方式，观察了大鼠90分钟运动后，耗竭运动后即刻和耗竭运动24小时后心肌线粒体脂质过氧化水平，抗氧化能力和游离钙含量的变化。发现：90分钟运动后心肌线粒体脂质过氧化水平，抗氧化能力和游离钙含量未见明显变化。而耗竭性游泳后心肌线粒体脂质过氧化水平显著提高，抗氧化能力和游离钙含量显著下降，24小时皆基本恢复。结果揭示耗竭运动时，机体内源性自由基产生，抗氧化能力下降是运动性疲劳和心肌损伤的原因之一。     

高水温条件下70分钟游泳运动对小鼠自由基代谢影响的研究

在46只清洁级纯种昆明小白鼠上进行了不同水温环境下,70分钟游泳运动对小鼠体温以及脑、肝、腓肠肌和心肌组织MDA含量,超氧化物歧化酶(SOD)活性水平、总抗氧化能力及肌糖元和肝糖元含量的影响的研究.结果如下:1.安静组维持在较高水平,p<0.05(数值分别为:肝419.80±47.56NU/ml上升到483.18±38.55NU/ml;脑149.3±38.87NU/ml上升到181.25±9.74NU/ml;腓肠肌101.37±7.20NU/ml上升到122.05±10.97NU/ml;心肌325.52±48.16NU/ml上升到381.63±37.97NU/ml),同时脑的MDA含量较安静组保持在较低水平,由安静组的6.01±2.26nm/ml下降为游泳组的4.59±0.65nm/ml(P相似文献   

人的衰老与自由基

衰老与自由基学说主要认为随着年龄增大的退行性变化是由于自由基的副作用引起。在正常情况下，机体内自由基的产生与消失是处于动态平衡的。当体内的自由基过剩时，细胞的生物大分子比学结构发生破坏性反应，损伤正常组织形态功能的完整性。当损伤程度超过修复或丧失其代偿能力时，组织器官的机能就逐步发生紊乱及障碍，表现出机体逐渐趋于衰老。     

Effects of α-tocopheryl acetate supplementation in preslaughter diet on antioxidant enzyme activities and fillet quality of commercial-size Sparus macrocephalus

This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (350±12) g] were divided into three groups (E250, E500 and E1000) and reared in 9 cages. The fish were fed for 8 weeks with three diets containing different levels of dietary α-tocopheryl acetate (289, 553, 1069 mg/kg). Over the experimental period, fish were fed to satiation and reached a final mean weight of (465±28) g without significant body weight difference and proximate composition difference. Fillet α-tocopherol was significantly (P＜0.05) different between groups, reaching levels of 14.2, 22.1, 30.9 μg/mg fillet for groups E250,E500 and E1000, respectively. Total serum superoxide dismutase (SOD) activity increased significantly (P＜0.05) in fish fed the diets high in α-tocopheryl acetate, but serum glutathione peroxidase (GPX) activity was unaffected. In storage on ice, fillets offish fed the diets high in α-tocopheryl acetate exhibited significantly lower (P＜0.05) levels of oxidation. These results suggested that increased dietary α-tocopheryl acetate could increase its flesh deposition, increase the activity of SOD and prevent lipid peroxidation of Sparus macrocephalus fillets in retail storage on ice.