Hyperinsulinemia and overweight in obese Zucker rats effectively suppressed by exercise training with hypoxia recovery

Abstract

It is currently unknown whether hypoxia training can effectively suppress overweight and hyperinsulinemia in genetically obese animals. In this study, both lean and obese Zucker rats were randomly assigned into the following groups: control (CON, n=7), exercise training (EX, n=7), hypoxia (HYP, n=7) and exercise training with hypoxia recovery (EX+HYP, n=7). During a 6-week training period, rats performed swimming exercise progressively from 30 to 180 min·day^?1, and recovered under hypoxia (14% oxygen for 8 h·day^?1). Obese Zucker rats exhibited substantially greater fasting insulin levels, and exaggerated glucose and insulin responses following an oral glucose challenge compared with lean rats. At the beginning of week 6, body weight, fasting glucose, fasting insulin, area under curve of glucose (GAUC) and insulin (IAUC) in the EX+HYP group were significantly lower than CON group among the obese rats. Meanwhile, only GAUC was significantly lower in the EX group compared to the CON group. At the end of week 6, capillaries to fibre ratio (C/F), capillary density (CD) and type IIa fibre proportion of the plantaris muscle in the EX group were significantly greater than the CON group (P<0.05), but no additive effect of hypoxia on exercise training was observed. Our data demonstrate that exercise training with prolonged hypoxia recovery offers better metabolic benefits than exercise training alone for the obese Zucker rats. This advantage was closely associated with effective weight reduction.     

Book reviews

Thigh muscle volume is a useful determinant of functional fitness. However, anthropometric prediction of muscle content is influenced by the variability of adipose tissue accumulation. The aims of this study were to predict thigh muscle and adipose tissue volumes from anthropometry and to assess the validity of the method by examining the various components of the measurements and the assumptions involved. The 19 participants (9 men, 10 women; age 23-49 years) varied in adiposity. They all underwent magnetic resonance imaging (MRI) of the upper leg and the eight men and two women with the lowest adiposity underwent detailed anthropometry involving girths and skinfolds. Using MRI as the reference method, muscle volume was predicted from anthropometry using a circular concentric model, and the assumptions inherent in the method were tested further using the MRI data alone. Muscle volume was best predicted by anthropometry in the 10 leanest participants using a five-slice truncated cone model that overestimated the mean MRI value by 30% ( R 2 = 0.95; standard error of estimate = 288 cm 3 ; P ? 0.001). A single skinfold plus girth measurement at the mid-thigh almost matched its predictive ability, but with an increased bias. Measurements of leg circumference by means of the two techniques agreed well. The assumption of a circular cross-section was valid. In contrast, the agreement between skinfold thickness measured by caliper and superficial adipose tissue thickness by MRI was poor, contributing to the scatter of fat and lean area comparisons. An anterior skinfold thickness measurement underestimated the area of superficial adipose tissue at that level, particularly at the most proximal and distal sites. Although these limitations increase the uncertainties of muscle volume determination by anthropometry, they do not prevent its valid prediction in leaner individuals. The prediction of superficial adipose tissue was poorer.     

Back injuries and the fast bowler in cricket

Here, I review research that has investigated the aetiology of injuries experienced by adolescent and adult fast bowlers. Mechanical factors play an important role in the aetiology of degenerative processes and injuries to the lumbar spine. This is particularly so in fast bowling, where a player must absorb vertical and horizontal components of the ground reaction force that are approximately five and two times body weight at front-foot and rear-foot impact, respectively. Attenuated forces are transmitted to the spine through the lower limb, while additional foces at the lumbo-sacral junction are caused by trunk hyperextension, lateral flexion and twisting during the delivery stride. Fast bowlers are classified as side-on, front-on or mixed. The mixed action is categorized by the lower body configuration of the front-on action and the upper body configuration of the side-on technique. This upper body configuration is produced by counter-rotation away from the batsman in the transverse plane about the longitudinal axis of the body of a line through the two shoulders. Counter-rotations of 12–40° during a delivery stride have predicted an increased incidence of lumbar spondylolysis, disc abnormality and muscle injury in fast bowlers. During the delivery stride, the mixed bowling action also shows: more lateral flexion and hyperextension of the lumbar spine at front-foot impact, and a greater range of motion of the trunk over the delivery stride when compared with the side-on and front-on techniques. The pars interarticularis of each vertebra is vulnerable to injury if repetitive flexion, rotation and hyperextension are present in the activity. Fast bowlers should reduce shoulder counter-rotation during the delivery stride to reduce the incidence of back injuries. When a player is required to bowl for extended periods irrespective of technique, overuse is also related to an increased incidence of back injuries and must be avoided.     

The relationship between torque and joint angle during knee extension in boys and men

The length-tension relationship of muscle contraction is well documented in adults. However, research on this relationship in children has been limited. The aim of this study was to compare differences in the torque-joint angle relationship of the quadriceps muscle in children and adults. Eight boys aged 8-10 years and eight men aged 20-26 years performed two maximal voluntary isometric contractions at six knee joint angles (20°, 40°, 60°, 80°, 90°, 100°). The mean of the two trials was used as the performance measure. Both groups demonstrated an expected increase in relative torque as the joint angle increased (P ? 0.05). The men produced significantly greater relative torque at 20°, 40° and 60° knee flexion (P ? 0.05). The percentage of maximal torque at these angles for the men and boys respectively were: 35.2 - 4.3 vs 15.2 - 12%, 63.6 - 9.1 vs 51.8 - 16.8% and 93.6 - 6.5 vs 84.4 - 14.4%. There were no group differences at 80° or 90°. Peak torque was attained at 80° in men, but decreased significantly (P ? 0.05) at 90° and 100°. For boys, peak torque was attained at joint angles of 80° and 90°. The reduction in peak torque at 100° was not statistically significant, but the relative torque at this angle was lower in men than in boys (77.9 - 13.7 vs 87.1 - 10.4%; P ? 0.05). In conclusion, the relationship between torque and joint angle appears to be affected by age.     

Effect of creatine supplementation on phosphocreatine resynthesis,inorganic phosphate accumulation and pH during intermittent maximal exercise

In this study, we examined the effect of creatine ingestion on muscle power output, muscle phosphocreatine resynthesis, inorganic phosphate and pH during repeated brief bouts of maximal exercise. Nine healthy males performed maximal plantar flexion before and after creatine ingestion (20 g ·day -1 for 6 days). The experimental protocol consisted of five 8 s bouts (bouts 1-5) interspersed with 30 s recovery, followed by bouts 6 (8 s) and 7 (16 s) separated by 1 and 2 min, respectively. Muscle phosphocreatine, inorganic phosphate and pH were estimated every 16 s by 31 P magnetic resonance spectroscopy. After creatine ingestion, muscle power output increased by ~5% ( P ? 0.05) from bouts 3 to 7 and muscle phosphocreatine resynthesis increased ( P ? 0.05) during 10 min recovery. The higher phosphocreatine concentration observed after only 30 s of recovery was accompanied by lower inorganic phosphate accumulation and higher pH. Strong correlations were found between exercise power restoration and the corresponding pre-exercise phosphocreatine and inorganic phosphate concentrations and muscle pH after creatine ingestion. The better maintenance of muscle power output observed after creatine ingestion was attributed to a higher rate of phosphocreatine resynthesis, lower accumulation of inorganic phosphate and higher pH.     

Effects of inspiratory muscle training on time-trial performance in trained cyclists

We evaluated the effects of specific inspiratory muscle training on simulated time-trial performance in trained cyclists. Using a double-blind, placebo-controlled design, 16 male cyclists (VO 2max = 64 - 2 ml·kg -1 ·min -1 ; mean - sx ¥ ) were assigned at random to either an experimental (pressure-threshold inspiratory muscle training) or sham-training control (placebo) group. Pulmonary function, maximum dynamic inspiratory muscle function and the physiological and perceptual responses to maximal incremental cycling were assessed. Simulated time-trial performance (20 and 40 km) was quantified as the time to complete pre-set amounts of work. Pulmonary function was unchanged after the intervention, but dynamic inspiratory muscle function improved in the inspiratory muscle training group ( P h 0.05). After the intervention, the inspiratory muscle training group experienced a reduction in the perception of respiratory and peripheral effort (Borg CR10: 16 - 4% and 18 - 4% respectively; compared with placebo, P h 0.01) and completed the simulated 20 and 40 km time-trials faster than the placebo group [66 - 30 and 115 - 38 s (3.8 - 1.7% and 4.6 - 1.9%) faster respectively; P = 0.025 and 0.009]. These results support evidence that specific inspiratory muscle training attenuates the perceptual response to maximal incremental exercise. Furthermore, they provide evidence of performance enhancements in competitive cyclists after inspiratory muscle training.     

Strength training: Isometric training at a range of joint angles versus dynamic training

Strength training with isometric contractions produces large but highly angle-specific adaptations. To contrast the contractile mode of isometric versus dynamic training, but diminish the strong angle specificity effect, we compared the strength gains produced by isometric training at four joint angles with conventional dynamic training. Thirty-three recreationally active healthy males aged 18?–?30 years completed 9 weeks of strength training of the quadriceps muscle group three times per week. An intra-individual design was adopted: one leg performed purely isometric training at each of four joint angles (isometrically trained leg); the other leg performed conventional dynamic training, lifting and lowering (dynamically trained leg). Both legs trained at similar relative loads for the same duration. The quadriceps strength of each leg was measured isometrically (at four angles) and isokinetically (at three velocities) pre and post training. After 9 weeks of training, the increase in isokinetic strength was similar in both legs (pooled data from three velocities: dynamically trained leg, 10.7%; isometrically trained leg, 10.5%). Isometric strength increases were significantly greater for the isometrically trained leg (pooled data from four angles: dynamically trained leg, 13.1%; isometrically trained leg, 18.0%). This may have been due to the greater absolute torque involved with isometric training or a residual angle specificity effect despite the isometric training being divided over four angles.     

Recovery of pulmonary diffusing capacity after maximal exercise

Abstract

Pulmonary diffusing capacity (Dl_co), together with spirometric variables, arterial oxygen tension (paO₂) and cardiac output were determined before and at intervals after maximal arm cranking, treadmill running and erogmeter rowing. Independent of the type of exercise, Dl_co increased immediately post‐exercise from a median 13.6 (range 7.3–16.3) to 15.1 (9.3–19.6) mmol min^‐1 kPa^‐1 (P <0.01). However, it decreased to 11.6 (6.9–15.5) mmol min^‐1 kPa^‐1 (P <0.01) after 24 h with cardiac output and paO₂ at resting values, and Dl_co normalized after 20 h. Thoracic electrical impedance at 2.5 and 100 kHz increased slightly post‐exercise, indicating a decrease in thoracic fluid balance, and there were no echocardiographic signs of left ventricular failure at the time of the decrease in Dl_co. Also, active muscle (limb) circumference and volume, and an increase in haematocrit from 43.8 (38.0–47.0) to 47.1 (42.7–49.8) (P <0.01), had normalized at the time of the decrease in Dl_co. Vital capacity, forced vital capacity, forced expiratory volume in 1 s, peak and peak mid‐expiratory flows did not change. However, total lung capacity increased from 6.8 (5.0–7.6) to 7.0 (5.1–7.8) litres (P <0.05) immediately after exercise and remained elevated at 6.9 (5.1–8.7) litres (P <0.05) when a decrease in Dl_co was noted. The results demonstrate that independent of the type of maximal exercise, an approximate 15% reduction in Dl_co takes place 2–3 h post‐exercise, which normalizes during the following day of recovery.     

Morphology of the deltoid muscles in elite tennis players

Abstract

The aim of this study was to examine the deltoid muscle properties of the dominant and non-dominant arm of Greek professional male tennis players. Eight male tennis players (mean age 22.0 years, s = 3.2) were subjected to biopsy of the deltoid muscle of both arms. Adenosine triphosphate (ATPase) histochemistry and myosin heavy chain (MHC) composition were performed on the samples with homogenate electrophoresis. No significant differences were observed in the percentage of types I, IIa, IIab, and IIb muscle fibres between the deltoid muscles of the two arms. Types I, IIa, and IIx muscle fibres of the dominant and non-dominant deltoid muscles did not differ significantly for MHC isoform composition. Type IIab muscle fibres showed a similar cross-sectional area (CSA) percentage distribution between the two arms. The CSA percentage for types I, IIa, and IIb muscle fibers did not differ significantly between the dominant and the non-dominant arm. We conclude therefore that regular tennis training probably does not lead to any significant changes in the muscle fibre types of the dominant and non-dominant arms of elite tennis players.