基于微阵列技术的缺氧/复氧诱导下血管内皮细胞转录组分析

目的:应用全转录组芯片研究缺氧/复氧诱导下人脐静脉内皮细胞(HUVEC)的转录组轮廓。创新点:血管内皮细胞(VEC)缺氧/复氧损伤被视定为许多生理和病理过程中导致器官功能障碍的重要驱动因素。然而,其详细病理生理机制和基因表达谱信息尚未阐明。本研究首次应用全转录组芯片技术研究VEC缺氧/复氧诱导下的转录组轮廓。方法:采用缺氧孵育3h后复氧1h的HUVEC为缺氧/复氧组,同时常氧孵育的HUVEC为常氧对照组。应用含58 339条探针的全转录组芯片检测每组三个样本。对差异表达基因进行生信分析和功能验证。结论:本研究发现372个有意义的差异表达基因探针。相关基因涵盖多种途径和功能,例如氧自由基的产生、钙超载、炎症、糖脂代谢、内皮细胞增殖、分化、细胞骨架及通透性调节、细胞裂解、凋亡和血管生成。另外,实验进一步表明,差异表达基因pleckstrin同源样域家族A成员1(PHLDA1)的m RNA和蛋白质表达结果与微阵列结果一致。STRING分析发现,PHLDA1可能与差异表达基因SLC38A3、SLC5A5、Lnc-SLC36A4-1和Lnc-PLEKHJ1-1具有物理性和/或功能性相互作用,这有望揭示VEC在缺氧/复氧环境下长链非编码RNA(lnc RNA)的相关机制。     

历史与欲望——毕飞宇早期小说叙事的两个要素

毕飞宇早期小说大张旗鼓地对历史进行了先锋色彩浓厚的演绎。毕飞宇历史叙事的动因由“家族情结”和“文革情结”两方面构成,其传达的是对历史、语言和权力三者亲缘关系的哲学体认,其根本的落脚点则在于批判欲和生命自省。     

How a small family run business adopted Critical Reflection Action Learning using hand drawn images to initiate organisational change

In this account of practice I would like to share my experiences of facilitating a Critical Reflection Action Learning (CRAL) set with a small family run business, struggling to make change and expand their services due to the problems they encountered in separating their business lives from their family lives. The account I present here is based on a 12-session project I carried out with the organisation using a CRAL methodology as part of my Doctoral studies at the University of Hull Business School (Shepherd 2011). In this particular Action Learning project participants were invited to create hand drawn images which acted as representations of the problems they faced as a business. The images were used by set members in reflective conversations on the problems they were having. Each set member cycled through a process of drawing an image and reflecting upon its meaning using individual, group and critical reflection. The whole process enabled set members to gain new insights into their problems and provided them with the opportunity to devise new, more effective ways of tackling their problems and understanding the power dynamics underpinning them.     

清前期赣南家族文学评述

清前期赣南的家族文学颇为兴盛 ,宁都三魏、瑞金杨氏五家、信丰黄世成一门三代 ,会昌霞绮园诸君子、于都三宋、大余西江四戴、赣县钟氏梨园世家等人才辈出 ,在中国古代文学史上写下亮丽一笔。     

家庭暴力原因与防范

我国家庭暴力的深层原因是妇女的合法权益受到夫权侵害,相关立法不完善。防范家庭暴力:应充分发挥妇联在反家庭暴力方面的独特作用;确立与婚姻法相配套的惩治家庭暴力的实施细则;提供相配套的社会性的帮助措施;借鉴其他国家有关立法、执法中惩治家庭暴力的成熟经验;提高社会全体成员的法律意识。     

家族企业发展的深层原因探析

家族企业在中国大陆和海外华人企业中广泛存在着，中国传统社会的家族制度和家文化传统以及长期以来中国私营经济的少数派地位，对我国家族企业的形成起到了决定性的影响。家族企业的产生有着特定的社会、历史条件和文化背景，家族企业这种古老而常新的企业组织形式将有助于促进中国私营企业和私营经济的发展。     

我国助学贷款的动态博弈分析

运用博弈论的方法分析了在助学贷款实施的过程中，银行和学生贷款人在无约束条件下、家庭担保条件下和个人信用制度约束条件下双方的动态博弈过程，并得出在我国个人信用制度尚未建立条件下，家庭担保贷款是最有效的助学贷款制度安排。     

汉魏六朝望族与郡望形成

汉魏六朝是望族发展和大姓郡望形成的关键时期。望族发展固然与时代政治制度和政治格局的变迁、宗族势力的兴衰、望族与皇族之间及望族与望族之间的婚姻等关系密切，但望族是否具备内在的自觉、自立精神与文化优势，这才是决定其兴衰的根本动力。     

Spillover in the Academy: Marriage Stability and Faculty Evaluations

The literature on the relationship between work and family has shown that there is a spillover effect between both domains. In particular, research that has investigated the influence of home environment on work has shown that family instability affects work satisfaction and performance. This study investigates the spillover between family and work by examining the link between marital status and work performance across the three phases of marriage, divorce, and remarriage. Specifically, this article links marital status and work performance through a longitudinal analysis of a set of university teaching evaluations. A polynomial regression model was fit to the data and a cubic curve through the three periods of marriage, divorce, and remarriage was statistically significant. Implications of the study and areas for future research are discussed.     

走向贞元文坛宗主地位的阶梯－权德舆的家世背景及学术渊源考察

权德舆乃贞元坛的盟主,在当时影响巨大,元和坛的重要作家均蒙其提携,对元和学的发展起了推波助澜的作用,早年“孤弱”的权氏之所以登上坛盟主的地位,除其自身的聪明才智和不懈努力而外,其良好的家世背景,学术渊源也为其提供了可能。