无脑儿心脏的组织学观察与比较

目的:探讨无脑儿与正常胎儿心脏结构的差异,为畸形胎儿的心脏发育研究提供形态学方面的资料。方法:用H.E染色,通过光镜观察无脑儿及正常胎儿的心脏结构。结果:无脑儿心脏的蒲肯野细胞数量明显低于同龄正常胎儿。结论:无脑儿神经系统发育异常影响其心脏内蒲肯野细胞的发育。     

兔膝半月板滑膜被覆的组织学、组织化学及超微结构的研究

本文通过20支兔膝关节半月板的组织学、组织化学和特染（VG.Mal-lory,Weigert,PMF,PAS,AB,PAS-AB,Feulgen,VonKossaXiGomori等法）以及超微结构（TEM,SEM）,对被覆半月板表面的薄层组织结构性质进行研究,较有说服力地证实为滑膜组织。为长期以来认为半月板有滑膜被覆（但遭到有些学者反对）和运动医学界（含矫形外科）对半月板损伤不需作手术切除,有自行愈合可能等提供形态学理论依据。     

空气阴极法制备过氧化氢用的电解槽

报道折流式降膜型空气阴极电解槽的结构和操作方法.该电解槽用于制备过氧化氢的试验结果表明:电解槽的阴极电流密度可达400～500A/m~2,槽电压为2.0～2.5V,电流效率为80％.     

Enzyme dynamic study on calmodulin Ca++-Mg++-ATPase system of red blood cells inhibited by α-anordrin and probimane

To clarify the possible mechanisms of α-anordin and probimane on calmodulin Ca⁺⁺-Mg⁺⁺ ATPase system, enzyme dynamic study was carried out by determining three dynamic parameters [the substrate concentration (ATP)-response curve, dose (inhibitors)-response curve and time-response curve]. Our data have shown that the inhibitory rates of α-anordrin and probimane are unrelated to substrate (APT) concentrations, but related to calmodulin concentrations. The inhibition of α-anordrin and probimane is very quick that is completed within 1 to 5 min and can maintain more than 1 hr in the same inhibitory rates. So it is possible that α-anordrin and probimane are calmodulin competitors with calmodulin-like binding whose actions can occur by affecting the reaction balance of substrate and product on target enzymes of calmodulin (Ca⁺⁺-Mg⁺⁺-ATPase).     

人白血病细胞系KG1a中P2X7受体的表达和功能研究

用半定量RT-PCR和流式细胞术,研究了白血病细胞系KG1a中P2X7受体在基因和蛋白水平的表达。用荧光分光光度计,测定了用激动剂三磷酸腺苷（ATP）和苯甲酰苯甲酸ATP（BzATP）刺激前后细胞内钙离子浓度的变化,证明P2X7受体的功能。结果表明：KG1a细胞表达P2X7受体,且在激动剂的刺激下能引起KG1a细胞通过P2X7受体的胞外钙内流;去除胞外钙离子时,激动剂不能引起胞内钙浓度的升高;提示KG1a细胞表达P2X7受体的基因和功能蛋白,激活该受体引起胞外钙离子的内流。     

胎盘源细胞治疗急性辐射骨髓衰竭研究

核辐射易造成人体造血系统损伤,为此建立急性辐射骨髓衰竭小鼠模型,通过单次腹腔注射异基因小鼠多份不同胎盘源细胞／细胞因子,明确其治疗急性辐射骨髓衰竭的疗效．实验将造模后小鼠随机分为胎盘源细胞治疗组、胎盘源细胞因子治疗组、胎盘源细胞联合细胞因子治疗组以及模型小鼠对照组4组,治疗后统计分析各组小鼠存活时间．结果表明,胎盘源细胞联合细胞因子可有效延缓急性辐射骨髓衰竭小鼠的寿命（P ＜0．05）．     

On the vein-stiffening membrane structure of a dragonfly hind wing

Aiming at exploring the excellent structural performance of the vein-stiffening membrane structure of dragonfly hind wings, we analyzed two planar computational models and three 3D computational models with cambered corrugation based on the finite element method. It is shown that the vein size in different zones is proportional to the magnitude of the vein internal force when the wing structure is subjected to uniform out-of-plane transverse loading. The membrane contributes little to the flexural stiffness of the planar wing models, while exerting an immense impact upon the stiffness of the 3D wing models with cambered corrugation. If a lumped mass of 10% of the wing is fixed on the leading edge close to the wing tip, the wing fundamental fre- quency decreases by 10.7%-13.2%; if a lumped mass is connected to the wing via multiple springs, the wing fundamental frequency decreases by 16.0%-18.0%. Such decrease in fundamental frequency explains the special function of the wing pterostigma in alleviating the wing quivering effect. These particular features of dragonfly wings can be mimicked in the design of new-style reticulately stiffening thin-walled roof systems and flapping wings in novel intelligent aerial vehicles.     

蓝尾蝾螈血液的组织学观察

本文对蓝尾蝾螈(Cynops cyanurus)血液进行显微观察、计数、细胞大小测量及数量比例测定。结果:红细胞较大,椭圆形,平均直径25.50×38.09μm,细胞核染色质密集,白细胞圆形,直径比红细胞长径短,核较大,白细胞中淋巴细胞最多,其次为中性粒细胞和单核细胞,酸性粒细胞极少,碱性拄细胞未见到,红细胞平均数为7.47×10~4/mm~3,白细胞平均数为0.80×10~3/mm~3,并与新疆北鲵和大鲵进行了比较和讨论。     

细胞分子生物学实验教学中显微数码互动系统的应用

在高校的实验课堂中,通过显微数码互动系统的应用,把光学显微镜、计算机及网络技术进行了结合,并在课堂中充分利用,实现了师生之间的直观交流、充分互动以及资源共享,能明显提高教学质量和效率。     

破伤风类毒素单克隆抗体的制备及初步鉴定

目的：制备并鉴定破伤风类毒素单克隆抗体杂交瘤细胞株,为破伤风的快速检测及致病机制的研究提供实验材料。方法：用破伤风类毒素做抗原免疫BALB/C小鼠,取其脾细胞与小鼠骨髓瘤细胞融合,获得分泌高滴度针对破伤风类毒素的杂交瘤细胞株,测定单抗免疫球蛋白亚类及单抗效价,用间接ELISA和Western-blot检测单克隆细胞株的特异性。结果：通过细胞融合和克隆化,筛选出3株持续分泌抗破伤风类毒素单克隆抗体的杂交瘤细胞株1E12、1E11、1G10。间接ELISA和Western-blot检测结果表明1E12、1E11、1G10可以和破伤风类毒素发生特异性反应。结论：成功制备了抗破伤风类毒素单克隆抗体,为制备免疫诊断试剂盒和抗体药物的开发奠定了基础。