A Tutorial on Centering in Cross-Sectional Two-Level Models

The primary purpose of this tutorial is to succinctly review some options for, and consequences of, centering Level 1 predictors in commonly applied cross-sectional two-level models. It is geared toward both practitioners and researchers. A general understanding of multilevel modeling is necessary prior to understanding the subtleties of centering decisions. A review of some high-quality journals within the broad discipline of exercise science provides evidence that multilevel modeling is used relatively infrequently in this field. Therefore, a secondary purpose is to introduce Measurement in Physical Education and Exercise Science readers to some core facets of multilevel modeling within the framework of this tutorial. A relevant dataset is used to demonstrate potential consequences of different centering decisions within a multilevel model. Depending on the model and the data, different centering decisions can exert non-trivial influence on the meaning of some model parameters, results from fitting the model, and subsequent conclusions.     

Age-Related Corresponding Relationships of Controlled Force Exertion Measured by a Computer-Generated Sinusoidal and Quasi-Random Display

This study examined age-group corresponding relationships of the controlled force exertion based on sinusoidal and quasi-random waveforms in 175 right-handed male adults aged 20 to 86 years. The subjects were divided into 3 groups based on age-level: 53 young (mean age 24.6, SD?=?3.3 years), 71 middle aged (mean age 44.3, SD?=?8.7 years), and 51 elderly (mean age 69.3, SD?=?6.4 years). The participants matched their submaximal grip strength by the dominant hand to changing demand values displayed as either a sinusoidal or a quasi-random waveform appearing on the display of a personal computer. The quasi-random waveform was changed in π with amplitude and in π/2 with frequency (peak and mean frequency were 0.1 Hz in both waveforms). The participants performed the controlled force exertion test three times with a 1-min interval (one trial was 40 sec) after one practice trial using the dominant hand. The measurement order was randomly assigned. The total of the differences between the demand value and grip exertion value for 25 sec were used as an evaluation parameter. The coefficient of variance was almost the same range in all age groups in both waveforms (CV_SW ?=?30.8–35.6, CV_RW ?=?26.3–37.7), but the elderly group showed the highest value in the quasi-random waveform. Significant correlations were found among the sinusoidal and quasi-random waveforms in the young, middle-aged, and elderly groups, but significant differences were not found in the correlations among each age group. The controlled force exertion evaluation based on the sinusoidal and quasi-random waveform displays has a moderate relationship, and this relationship does not show age-level differences.     

Functional capacities and sport-specific skills of 14- to 15-year-old male basketball players: Size and maturity effects

Abstract

The aim of the study was to estimate the influence of body size and pubertal status on variation in functional capacities and sport-specific skills of 59 youth basketball players aged 14.0–15.9 years. Height and mass were measured and stage of pubic hair was assessed at clinical examination. Six tests of functional capacity were evaluated: squat jump, countermovement jump, 60-s sit-ups, 2-kg standing medicine ball throw, hand grip strength, and 20-m multi-stage shuttle run. Four basketball skills were tested (shooting, passing, dribbling, and defensive movements). Comparisons between basketball players of different sexual maturity status were performed using analysis of covariance (controlling for chronological age). Functional capacities and basketball skills appeared to be largely independent of pubertal status especially after controlling for variation in body size. Results of multiple linear regressions indicated chronological age as a significant predictor for four items, while maturity status was a significant predictor for only one item. The influence of body mass was negative for two functional indicators (jumping, multi-stage shuttle run) and two basketball skills (dribbling, defensive movements), but positive for two functional tests of upper body strength (hand grip, ball throw). Height was positively correlated with two specific skills (passing, defensive movements), while a combination of tallness and heaviness was associated with a disadvantage on three functional capacities and two sport-specific skills.     

Prophylactic effect of hot pack on symptoms of eccentric exercise-induced muscle damage of the wrist extensors

Abstract

This study investigated whether hot pack treatment could provide prophylactic effects on muscle damage induced by eccentric exercise of the wrist extensors. Twenty-eight healthy men (age 21±1 years, weight 65±16 kg, height 171±6 cm) were randomly placed into hot pack (n = 14) and control (n = 14) groups. All participants performed an exercise consisting of 300 maximal eccentric contractions of the wrist extensors of the non-dominant arm using an isokinetic dynamometer. A hot pack was applied for 20 min to the wrist extensors of the exercised arm before the exercise for the hot pack group. The control group received no treatment before the exercise. Measured variables included pain intensity assessed by a visual analogue scale and a modified Likert's scale, cold thermal pain threshold, pressure pain threshold (PPT), range of motion in active wrist flexion (ROM-AF) and extension (ROM-AE), range of motion in passive wrist flexion (ROM-PF) and extension (ROM-PE), grip strength, and wrist extension strength. Changes in these variables before, immediately after, and 1 to 8 days following the exercise were compared between groups by a two-way repeated measures ANOVA. All outcome measures from both groups (except for the cold thermal pain threshold of the hot pack group) demonstrated a significant change within the first 2–3 days following exercise. Significant differences between groups were only found at a single point in time for PPT, ROM-PF, ROM-PE and ROM-AE, and the changes were smaller for the hot pack group in comparison to the control group. These results suggest that the prophylactic effects of hot pack treatment on eccentric exercise-induced muscle damage of the wrist extensors are limited.     

Water immersion as a recovery aid from intermittent shuttle running exercise

Abstract

Aim: To investigate the effect of different water immersion (WI) treatments on recovery from intermittent shuttle running exercise in comparison to an ecologically relevant control.

Methods: Forty males performed 90 minutes intermittent shuttle running, following which they were assigned to either: (1) 12-min standing WI at 12°C; (2) 12-min standing WI at 35°C; (3) 2-min seated WI at 12°C; (4) an ecologically relevant control consisting of 12 minutes walking at 5 km h^?1. Muscle soreness, maximal voluntary contraction (MVC) of the knee flexors and extensors, hop distance, creatine kinase activity and myoglobin concentration were measured before exercise and in the 168 hours following the intervention. Between-group differences, time effects and interaction effects were investigated by mixed-model ANOVA.

Results: The shuttle running exercise induced an increase in muscle soreness (1, 24, 48 and 72 hours post-intervention) creatine kinase activity and myoglobin concentration (post-exercise and 1, 24 and 48 hours post-intervention), and reduced MVC of the knee extensors (11% reduction at 24 hours, remaining reduced at 48 and 72 hours), flexors (24% reduction at 24 hours, remaining reduced at 24, 48, 72 and 168 hours), and hop distance (24 and 48 hours). However, no between-group differences or interaction effects were evident for any of these parameters.

Conclusion: The WI protocols investigated were not better than light exercise in facilitating recovery from shuttle running exercise. Future studies examining the efficacy of WI as a recovery intervention should include a representative control condition to increase their relevance to sporting populations.     

No effect of upper body compression garments in elite flat-water kayakers

Abstract

While the effect of lower body compression garments on performance and physiological responses are well documented, no studies have examined the effect of upper body compression garments (UBCG) on upper-body dominant exercise. This study examined the effects of wearing UBCG on performance and physiological responses during simulated flat-water kayaking. Five male (mean values±s: 21.8±2.8 years; 83.5±9.2 kg; 63.0±5.5 ml·kg^?1·min^?1) and two female (mean values±s: 25.0±4.2 years; 71.4±2.7 kg; 51.0±4.8 ml·kg^-1·min^-1) elite flat-water kayakers completed a six-step incremental test followed by a four-minute maximal performance test (4minPT) in both UBCG and control (no shirt or sports training bra) conditions in a randomized counter-balanced order. Heart rate and oxygen consumption (O₂) as well as performance measures (power, distance covered, stroke rate) were recorded during the tests, and blood lactate was measured immediately after each incremental step and three minutes following the 4minPT. Near-infrared spectroscopy-derived measures of blood flow and oxygenation of the flexor carpi radialis were monitored continuously for all tests. No significant differences between the UBCG and control conditions were evident for any performance, cardiorespiratory or oxygenation measure across the incremental step test and 4minPT. It was concluded that wearing UBCG did not provide any significant physiological or performance benefits during simulated flat-water kayaking.     

Acute caffeine ingestion enhances strength performance and reduces perceived exertion and muscle pain perception during resistance exercise

Abstract

The efficacy of caffeine ingestion in enhancing aerobic performance is well established. However, despite suggestions that caffeine may enhance resistance exercise performance, research is equivocal on the effect of acute caffeine ingestion on resistance exercise performance. It has also been suggested that dampened perception of perceived exertion and pain perception might be an explanation for any possible enhancement of resistance exercise performance due to caffeine ingestion. Therefore, the aim of this study was to examine the acute effect of caffeine ingestion on repetitions to failure, rating of perceived exertion (RPE) and muscle pain perception during resistance exercise to failure. Eleven resistance trained individuals (9 males, 2 females, mean age±SD=26.4±6.4 years), took part in this double-blind, randomised cross-over experimental study whereby they ingested a caffeinated (5 mg kg^?1) or placebo solution 60 minutes before completing a bout of resistance exercise. Experimental conditions were separated by at least 48 hours. Resistance exercise sessions consisted of bench press, deadlift, prone row and back squat exercise to failure at an intensity of 60% 1 repetition maximum. Results indicated that participants completed significantly greater repetitions to failure, irrespective of exercise, in the presence of caffeine (p=0.0001). Mean±S.D of repetitions to failure was 19.6±3.7 and 18.5±4.1 in caffeine and placebo conditions, respectively. There were no differences in peak heart rate or peak blood lactate values across conditions (both p >0.05). RPE was significantly lower in the caffeine compared to the placebo condition (p=0.03) and was significantly higher during lower body exercises compared to upper body exercises irrespective of substance ingested (p=0.0001). For muscle pain perception, a significant condition by exercise interaction (p=0.027) revealed that muscle pain perception was lower in the caffeine condition, irrespective of exercise. With caffeine, pain perception was significantly higher in the deadlift and back squat compared to the bench press. However, with placebo, pain perception was significantly higher for the deadlift and back squat compared to the prone row only. Therefore, acute caffeine ingestion not only enhances resistance exercise performance to failure but also reduces perception of exertion and muscle pain.     

Effects of a resistance-training programme on endoplasmic reticulum unfolded protein response and mitochondrial functions in PBMCs from elderly subjects

Abstract

Aging has been related with a decline in the ability to handle protein folding, which leads to endoplasmic reticulum stress and alterations in unfolded protein response (UPR). Importantly, physical activity could activate the UPR and attenuate or prevent age-induced endoplasmic reticulum (ER) dysfunction. The current study evaluated the effects of a resistance exercise on UPR and mitochondrial functions in peripheral blood mononuclear cells (PBMCs) from elderly subjects. Thirty healthy women and men (age, 72.8, s_x??=?2.2 years) were randomized to a training group, which performed an 8-week resistance training programme, or a control group, which followed their daily routines. The phosphorylation of PERK and IRE1, as well as ATF4, and XBP1 protein expression, significantly increased following the training, while expression of BiP, AFT6 and CHOP remain without changes. Additionally, the intervention also induced an increase in PGC-1α and Mfn1 protein levels, while no changes were found in Drp1 expression. Finally, the resistance protocol was not able to activate PINK1/Parkin and Bnip3/Nix pathways. The results obtained seem to indicate that 8-week resistance exercise activates the UPR, stimulates mitochondrial biogenesis, maintains mitochondrial dynamics and prevents mitophagy activation by unfolded proteins in PBMCs from elderly subjects.     

Effects of high-intensity resistance circuit-based training in hypoxia on body composition and strength performance

Abstract

Hypoxic training methods are increasingly being used by researchers in an attempt to improve performance in normoxic ambients. Moreover, previous research suggests that resistance training in hypoxia can cause physiological and muscle adaptations. The primary aim of this study was to compare the effects of 8 weeks of high-intensity resistance circuit-based (HRC) training in hypoxia on body composition and strength performance. The secondary aim was to examine the effects of HRC on metabolic parameters. Twenty-eight male participants were randomly assigned to either hypoxia (Fraction of inspired oxygen [F_IO₂]?=?15%; HRC_hyp: n?=?15; age: 24.6?±?6.8 years; height: 177.4?±?5.9?cm; weight: 74.9?±?11.5?kg) or normoxia [F_IO₂]?=?20.9%; HRC_norm: n?=?13; age: 23.2?±?5.2 years; height: 173.4?±?6.2?cm; weight: 69.4?±?7.4?kg) groups. Training sessions consisted of two blocks of three exercises (Block 1: bench press, leg extension and front pull down; Block 2: deadlift, elbow flexion and ankle extension). Each exercise was performed at six repetition maximum. Rest periods lasted for 35-s between exercises, 3-min between sets and 5-min between blocks. Participants exercised twice weekly for 8 weeks, and body composition, strength and blood tests were performed before and after the training program. Lean body mass and bone mineral density significantly increased over time in the HRC_hyp (p?<?.005; ES?=?0.14 and p?<?.014; ES?=?0.19, respectively) but not in the HRC_norm after training. Both groups improved their strength performance over time (p?<?.001), but without group effect differences. These results indicate that simulated hypoxia during HRC exercise produced trivial effects on lean body mass and bone mineral density compared to normoxia.     

Sex-related differences in joint-angle-specific hamstring-to-quadriceps function following fatigue

Abstract

The purpose of this study was to examine the effect of fatiguing exercise on sex-related differences in the function of hamstring and quadriceps muscles at several angular velocities and joint angles. Physically active participants (50 male: 28.7?±?4.5y, 1.82?±?0.07 m, 82.3?±?6.87?kg; 50 female: 27.0?±?5.8y, 1.61?±?0.08 m, 68.75?±?9.24?kg) carried out an isokinetic assessment to determine concentric and eccentric torques during knee extension and flexion actions at three different angular velocities (60/180/300°/s). The H/Q_FUNCT was calculated using peak torque (PT) values at 3 different joint-angle-specific (15°, 30° and 45° of knee flexion). A repeated measures analysis of variance (ANOVA) was used to compare within group results. Between group comparisons of sex-related differences were assessed by independent T-tests. Fatiguing exercise in males resulted in a decrease in H/Q_FUNCT ratios for each angle of knee flexion at both 60°/s and 300°/s angular velocities (p?<?0.05). In females, significant decreases in H/Q_FUNCT ratios were observed following fatiguing exercise for each angle of knee flexion and angular velocity (p?<?0.01). Significant differences in H/Q_FUNCT ratios following fatiguing exercise were evident between males and females at each joint angle and angular velocity (p?<?0.01). These findings indicate sex related differences in H/Q_FUNCT ratios following fatiguing exercise. Females have greater reductions in torque and H/Q_FUNCT ratios following fatigue than their male counterparts. This potentially exposes females to higher risks of injury, particularly when fatigued. Practitioners should attend to the imbalance in fatigue resistance of hamstring and quadriceps function, particularly in female athletes.