假丝酵母Candidasp．发酵生产脂肪酶培养条件的优化

研究了碳源和氮源种类、碳源浓度以及起始pH值对假丝酵母Candidasp。菌株发酵生产脂肪酶的影响。结果显示,最适碳源为橄榄油,氮源为酵母膏,橄榄油浓度为2％,起始pH值为6.0。应用响应面试验设计对酵母膏、橄榄油和起始pH值进行培养基优化,结果表明,优化后的培养基配方为：橄榄油2.582％,酵母膏0.276％,K2HPO4 0.1％,MgSO4·7H2O 0.01％,NaCl 0.05％,pH6.28。在此条件下,假丝酵母Candidasp.液体发酵液中脂肪酶的酶活力可达36250U·mL^-1。     

Effect of temperature on batch elastase production by Bacillus sp. EL31410

The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value [5.5 U/(h-g DCW)] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/(h-g DCW) at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and os     

8种杀菌剂对苦瓜枯萎病菌的室内毒力测定

采用生长速率测定法和分生孢子萌发抑制率法,在舍药PDA平板上和凹玻片上分别测定8种杀菌剂对苦瓜枯萎病尖孢镰刀菌的毒力作用。结果表明：20％五氯硝基苯DP对菌丝生长的抑制作用最强,EC50值为8．37mg／L,其次为40％五硝·多菌灵WP、12．5％戊唑醇WP、50％多菌灵WP和75％百菌清、聊,EC50值分别为：10．61mg／L、10．65mg／L、12．62mg／L、15．40mg／L。12．5％戊唑醇WP和对孢子萌发抑制作用最强,EC50值为3．68,20％五氯硝基苯DP、25％腈菌唑EC、40％五硝·多菌灵Ⅵ巾、50％多菌灵WP次之,EC50值分别为4．81mg／L、8．51mg／L、9．90mg／L、11．17mg／L。     

由有限向无限的艺术扩张--论吴组缃短篇小说的艺术魅力

吴组缃的短篇小说以其探根究由的人物塑造、富于惯性的节奏、微言大义的笔法向人们展示了一个由有限向无限扩张的艺术空间,具有极强的艺术感染力.     

上、下楼梯时认知任务介入对下肢协调性影响的研究

目的：探讨上、下楼梯时附加认知任务,年龄差异（组间）及任务难度（组内）对步态时空及下肢关节协调参数的影响。方法：18位健康老年人为老年组,18位健康大学生为年轻组,各组内均为9男9女,依次接受认知任务测试、上、下楼测试（单任务）及上、下楼合并认知任务测试（双任务）。结果：认知任务方面,两种任务情境下各年龄组上楼回答次数皆显著多于下楼。步态参数方面,两种任务情境下年轻组相对于老年组,以及各年龄组在单任务情境下相对于双任务,皆呈现较短步行时间、较快步行频率、速度及较大步行长度的情形。下肢协调性方面,年轻组上楼过渡时摆动期的膝‐踝协调稳定度在双任务下较单任务差,而处于双任务情境下,年轻组上楼过程中支撑期的膝‐踝协调稳定度比老年组差;年轻组下楼过程中摆动期的髋‐膝协调稳定度在ST 和DT 中都比老年组差,老年组膝‐踝协调稳定度比年轻组差,下楼过渡时,年轻组支撑期髋‐膝协调稳定度在两种任务情境下皆比老年组差。结论：老年人和年轻人会根据任务难度来选择是否专注于认知任务上,此外年轻人因有足够下肢肌力控制而采取较大胆动作策略,导致其上下楼梯时下肢协调稳定度较差,而老年人则趋向选择较保守的策略来维持下肢关节协调稳定度。     

黄鳝体内毛细线虫的感染研究

本文以黄鳝体由毛细线虫的感染情况为重点，研究了毛细线虫的感染率、感染强度。结果表明：其感染率为13．3％，感染强度为3．25，平均密度为0．433；黄鳝的感染与其自身的体长有一定的关系，毛细线虫一般都集中在体长范围为200－350mm的黄鳝体内，其它体长组的黄鳝则很少感染。     

Characterization of an extracellular polysaccharide produced by Bacillus sp.RL-2

1 Introduction a Bioflocculation is a dynamic process resulting fromsynthesis of extracellular polymer by living cells.Flocculant are widely used in industrial processesincluding wastewater treatment, downstream pro-cessing, and food and fermentation processes [1-6].The flocculants used can be classified into three groups1) inorganic flocculants such as aluminum sulfate andpoly-aluminum chloride; 2) synthetic organic high-polymer flocculants such as polyacrylamide derivativesand polyethylene i…     

苦荆茶中多酚类物质提取方法的研究

采用比色法测定了用四种不同极性的溶剂提取的苦荆茶(Ilex．sp)中多酚类物质的含量，发现四种溶剂提取多酚类物质的得率高低依次是：丙酮水(10．10％)＞水(9．39％)＞60％乙醇(9．17％)＞95％乙醇(6．84％)；揭示了苦荆茶中多酚类物质与普通绿茶中茶多酚有相似的特性，易溶于丙酮水中。同时，也阐明了苦荆茶可能具有抗氧化，抗衰老和延年益寿的功效。     

PglnA启动子控制的ω-3去饱和酶基因的转基因集胞藻的构建

为了能在常温下提高多不饱和脂肪酸产量,以及用单交换的方式在集胞藻PCC6803的染色体上整合入基因片段,本研究首先构建出链霉素抗性基因、常温启动子、desB基因片段依次相连的质粒,然后以单交换的方式将这些片段整合在集胞藻Pcc6803的染色体上,最后成功筛选出了转基因藻.该研究为常温下大量表达多不饱和脂肪酸奠定了基础.     

中国葫芦科植物志资料——雪胆属

the present paper is a meterial of the genus Hemsleya Cogn. for flora of China-Cucurbitaceae.      The genus Hemsleya was established by C. A. Cogniaux in 1889.  By 1982 about 7 species had been discovered in China, and most of them are mainly distributed in S. W. China, particularly in Yunnan and Sichuan.  Their tubers have been used as a folk medicine for a long time.  However, we began to search for them, and meanwhile collect and cultivate them, only about ten years ago.  After a general survey and taxonomical study, 20 species of Hemsleya are recorded from Yunnan in the paper.  Among them 18 species and 3 varieties are new. All the types are kept in Herb. KUN.      According to the characters, of corolla and fruit also the seed condition, widely or narrowly winged, proposed are four sections, namely: Sect. I. Craciliflorae; Sect. II. Amabiles; Sect. III. Carnosiflorae and Sect. IV. Hemsleya, among which 3 are new.