优化的TTF-1核心启动子驱动miRNA-7表达有效抑制人非小细胞肺癌细胞的生长（英文）

靶向基因治疗是一种很有前景的肺癌治疗方法。在前期工作中,我们报道了由甲状腺转录因子-1(TTF-1)启动子调控微小RNA-7(miR-7)的靶向表达可在体外和体内抑制人肺癌细胞的生长,但干预效率有待进一步提高。在本研究中,我们通过5’缺失分析鉴定了TTF-1的核心启动子(从-1299 bp到-871 bp),并筛选出潜在的结合转录因子核因子-1(NF-1)和激活蛋白-1(AP-1)。进一步分析的结果表明：NF-1的表达水平,而非AP-1的表达水平,与人非小细胞肺癌(NSCLC)细胞中TTF-1核心启动子的活性呈正相关。此外,NF-1的沉默可以降低由TTF-1核心启动子调控的miR-7的表达。重要的是,我们在TTF-1核心启动子的序列上优化了四个不同的序列(称为^optTTF-1启动子)以形成额外的NF-1结合位点(TGGCA),并通过电泳迁移率实验(EMSA)分析验证了NF-1对^optTTF-1启动子的结合效率。通进一步研究,结果显示^optTTF-1启动子可更有效地驱动miR-7表达并在体外抑制人NSCLC细胞的生长,同时...     

基于OBB-SAT算法的双五轴加工路径碰撞检测方法

在双五轴镜像铣削加工过程中,支撑端和铣削端在同步运动时可能会发生碰撞,对设备安全和蒙皮件质量造成较大影响.为了预防两端之间的干涉,采用OBB包围盒模型与分离轴算法实现镜像铣加工的碰撞检测.首先分析OBB包围盒的建立方法及其优势,并建立机床支撑端与铣削端的包围盒模型;然后通过分离轴算法得到支撑端与铣削端的最近距离,并判断...     

与椭圆和双曲线轴上点相关的几个性质

本文从线段长度倒数和与差间的关系、直线斜率之积间的关系和直线斜率之商间的关系等3个角度出发,给出了与椭圆和双曲线轴上点相关的9个有趣的性质.     

一起顶轴油系统异常事故分析

通过一起转子不能顶起的事故分析,处理,分析了问题产生的原因。     

太极拳腰、胸、身、手略述

在太极拳习练中,以腰为轴,胸的含展,身手的运转,是为练习的根基,如何习练出功夫,要看习练者对太极拳这些要素吃透多少,运作如何。《十三势歌诀》起首即曰:"命意源头在腰隙"。就可知道腰隙在太极拳练习中的重要性。然而,大多数太极拳习练者不解腰隙何指,问至     

浅谈“移轴”公式和“移图”公式的教学

在中学数学中,"移图"公式与"移轴"公式两者容易混淆,运用公式解题容易出错.要区分开两者,必须从对照识记公式、剖析两者异同、熟练运用公式三方面入手.     

机车轴坯质量与生产技术分析

对轴坯实物的检验结果与试制技术进行了分析，证实了生产技术，检验技术的先进性、可靠性，实物质量满足技术标准要求，达到国际先进水平。     

愈甲胶囊的制备及质量控制

目的：探讨临床治疗甲状腺机能亢进的新药——愈甲胶囊的制备方法及质量控制方法。方法：利用古方当归六黄汤加栀子、板兰根,将传统汤剂通过现代加工技术制成胶囊剂。结果：本制剂组方合理,制备工艺可行,质量容易控制。结论：愈甲胶囊为疗效确切、质量稳定、服用方便、成份质量有标准的纯中药制剂,可满足临床用药需求。     

Investigation of iodine concentration in salt,water and soil along the coast of Zhejiang,China

Objective: We aim to describe the environment iodine concentration in salt, water and soil along Zhejiang Province coast in the China foreland. It will be helpful for us to judge whether this area is insufficient in iodine and universal iodized salt is necessary or not. Methods: We collected iodized salt samples, drinking water samples (tap water in the towns, and well water or spring water in the villages), water samples from different sources (ditches, lakes, rivers) and soil samples through random sampling in June, 2005. Salt, water and soil iodine was detected by arsenic-cerium redox method. Statistical analysis was expressed as mean±SEMby Windows SPSS 13.0. Results: (1) The iodine concentration in salt was 27.9±4.33 mg/kg (n=108). (2) Seventy-five water samples were collected. The water iodine value was 0.6～84.8 μg/L (mean of 11.66 μg/L). The watershed along the Qiantang River has significantly higher iodine content than the water in Lin'an in mountain area (P＜0.01). The iodine content and mean iodine content of tap water, well or spring water and natural water sources were 4.30±2.43 μg/L (n=34), 23.59±27.74 μg/L (n=19)and 12.72±10.72 μg/L (n=22) respectively. This indicated that among environmental water sources, the ditch iodine content was the highest with river water iodine being the lowest (P＜0.01). (3) Soil iodine value was 0.11～2.93 mg/kg (mean of 1.32 mg/kg).Though there was no statistical difference of soil iodine in different districts (P=0.131), soil iodine content correlated positively with water iodine content. Conclusion: Iodine concentration in salt accords with national policy of adding iodine in salt. Foreland has more iodine in water than mountain area. The data reflected that water and soil iodine in foreland area was not high, which suggests universal iodized salt should be necessary. Environment iodine has relatively close association with pollution.