马王堆“导引术”与舞蹈的中医养生价值探析*

马王堆"导引术"源自汉墓出土的帛画《导引图》中的图式,是迄今为止我国流传最广的一套用来保健与治疗疾病的养生功法;舞蹈作为一种人体动作的艺术,远古时代已被运用为导引养生手段.从梳理马王堆"导引术"与舞蹈养生同源、同功的渊源关系出发,运用中医哲学思想的"整体观念"理论,通过研究其形与神俱、精气神、经络说的养生观点,揭示了基于两者保健预防、祛病治疗、心理调剂的中医养生的实践价值.     

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.     

人体有氧能力的研究

本文概述了国内外有关儿童少年最大吸氧量的自然增长规律及其性别差异；并着重阐述了人体有氧能力与耐力运动项目成绩的关系。并根据研究数据和国内外学者的同类研究的数据，进行非线性回归模型的最小二乘拟合，建立了予测13～岁男性最大摄氧量的公式。本文还首次提出了最大摄氧量平台（VO2maxPD）的概念，进而深入的讨论了VO2max、VO2maxPD和VO2AT三者的关系。     

基于SNA核心技术领域测度研究

测度一个产业的核心技术领域,将对产业R&D资金投入决策和科技人力资源配置具有重要的理论意义和现实意义。社会网络分析方法可以用来进行核心技术领域的探测分析;网络中心度指标,可以作为测度核心技术领域的一项重要指标。对2009年全球航空航天产业专利进行技术网络分析的结果显示,全球航空航天产业的核心技术主要集中在通讯技术领域。     

维普数据库Z39.50服务端系统设计方案

介绍了维普数据库Z39.50服务端系统的设计方案，该方案有两条解决途径：基于TCP/IP的Z39.50服务端系统和基于HTTP的SRU服务端系统，笔者将这两种思路做了比较。本系统突破了传统Z39.50协议只运用于对书目数据的提供，而是提供电子期刊数据，这是本方案的特色所在。     

知识团队中变革型领导对组织公民行为的影响

本文用实证研究的方法探讨了我国高科技组织的知识工作团队中团队领导者的变革型领导行为对团队成员组织公民行为的影响作用及影响机制。通过对来自四个城市的12家高科技组织的52个知识工作团队的领导者和294名团队成员的调查,研究结果发现:变革型领导行为对团队成员的组织公民行为具有显著的影响作用;关系导向的变革型领导行为对成员的组织公民行为影响作用更为强烈;领导-成员交换在变革型领导和团队成员组织公民行为之间起到部分中介作用。     

天门冬药理与临床应用研究进展

天门冬药用价值高、作用明确、毒副作用少,是一种重要的药用植物,为多个民族的常用药,极具开发前景.综述了目前天门冬药理作用与临床应用的研究进展,并对未来的研究作了展望.     

浅谈中国街头篮球的发展现状及其对中国职业篮球发展的影响

运用文献资料和逻辑分析法,研究街头篮球起源及发展的文化背景,分析中国街头篮球的发展历程、现状及发展方向,探讨其对中国职业篮球发展的影响。     

D406A钢K_(Ic)与动态力学性能的相关性研究

为总结D406A钢的静态断裂韧性KIc值与动态力学性能间的关系,分别采用三点弯曲试样以及标准冲击试样对D406A钢进行了KIc测试和仪器化冲击试验,发现室温下该材料的冲击功CVN与断裂韧度KIc存在着相同的变化趋势,两者间的对应关系与修正后的Rolfe-Novak经验公式基本相符,今后可以通过该公式来推断材料的KIc值。利用标准冲击样所获得的动态断裂韧度KId与静态断裂韧度KIc虽遵循相同的变化规律,但相关性不是特别理想,这主要是由于一方面动态加载条件下试样的受力情况远比静态下要复杂,这更容易导致KId的离散;另一方面标准冲击样并不是一个理想的裂纹体,缺口尖端在断裂之前会普遍屈服,这也会给KId的计算带来较大误差。     

Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.