一种求解非线性方程组的混沌优化算法

本文针对非线性方程组的求解问题提出一种混合算法,将方程组转换成一个优化问题。利用优化问题的非线性共轭梯度法与混沌优化方法相结合,提出了一种新的混合优化算法。该算法能使非线性共轭梯度法跳出局部最优,最终获得全局最优。算法的收敛性也进行了证明,数值结果表明该算法是有效的。     

基于数值信息法的煤巷支护综合设计研究

针对山西大同某煤矿辅助巷道支护结构的锚杆与锚索的设计,采用理论设计、数值模拟、观测设计、工程类比几种方法的综合设计进行方案选择、设计优化。此方案不仅在经济方面,而且在技术、安全方面都满足要求,对类似的工程起到借鉴的作用。     

Study on the submerged fermentation (SMF) of termitomyces eurrhizus

The influence of KH\-2PO\-4, peptone and brown sugar on Termitomyces fuliginosu's mycelium formation was studied with the application of the design of quadratic rotation general combination based on determining the growth curve. A quadratic regression model of biomass to the doses of the above three factors was established. The model fit well and therefore the optimum fermentation condition was obtained. Responses of biomass to the three single factors and to their interactions were discussed. Thus, the highest level of biomass, 21.1 g/L, appeared under the optimized conditions when the initial KH\-2PO\-4, peptone and brown sugar were 1 g/L, 4 g/L and 72 g/L respectively.     

L-fuzzy相对T-1,相对T0与相对次T0分离性

定义了L-fuzzy拓扑空间中的相对T-1，相对T0与相对次T0分离性，讨论了相对T-1，相对T0与相对次T0分离性的一系列性质．证明了相对T-1，相对T0与相对次T0分离性是遗传的、传递的、弱同胚不变的及可乘性等性质，并给出了一些例子。     

多媒体技术在教学中的作用

教师应充分认识多媒体技术的优势并积极运用,以提高教学效率;通过研究多媒体技术与教学要素之间的关系,要求教师科学而有效地应用多媒体技术,以实现教育教学目标的最优化。     

免疫算法与遗传算法的比较

免疫算法与遗传算法二者有不同点，通过仿真实验表明它们之间在功能和应用方面有区别．     

合成草莓酯的新型催化剂

综述了无机盐、无机酸、杂多酸(盐)、固体超强酸等催化剂催化合成草莓酯的实验情况.结果表明,硫酸铝、CuCl2 ·2H2 O、固体超强酸SO2 -4 -TiO2 /Al2 O3、活性炭固载硅钨酸等催化剂催化合成草莓酯的收率较高,具有一定的工业应用价值.     

科技期刊编辑心理素质结构及其优化

科技期刊编辑心理素质结构主要是性格品质中的态度、情感和意志，心理能力中的认知能力和心理适应能力，心理动力中的动机和兴趣等。提出了优化编辑心理素质的途径：塑造良好的性格品质，培养高水平的心理能力，激发强烈的心理动力。     

R&D co-operation and innovation a microeconometric study on French firms

The scope of this paper is to report new empirical evidence on the determinants of R&D co-operation. Indeed, the literature on the capabilities of firms emphasises the role of knowledge in the performance and evolution of firms who use knowledge developed in others to build their own knowledge capital. R&D co-operation between firms is one of the many strategies by which this knowledge may be transmitted. Several theoretical models have stressed that R&D co-operation is more likely when the level of spillovers is high. While this supposition is used in many theoretical models, it has rarely been tested before. Our results do not lend strong support to this intuition. Indeed, our spillover variables (national pure spillovers, national rent spillovers and imports of machine tools), which were supposed to match the theoretical notion of spillovers used in these theoretical models have a positive but not a significant role when explaining R&D co-operation. The R&D co-operation increases with size and with R&D intensity, but not with market share. It also increases with the budget spent on paying license fees and on acquiring patents and labour from foreign firms. In this study, we also analyse the determinants of innovation. In more precise terms, we compare the effects of R&D co-operation to those played by traditional internal factors and those exerted by external, pure and rent spillovers on the innovation capacity of the firms. Our results show that the commercial success of innovations of French firms depends mainly on size, market share, R&D intensity and human capital. Inward FDI from industrialised countries exerts a positive and significant effect. The finding highlights the important role of the absorptive capacity of firms. Spillover measurements, such as the acquisition of machine tools, foreign patents, licenses, and technological opportunities have a positive impact on innovation.     

Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro

BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006