国外首部《淮南子》英语全译本研究

《淮南子》成书于西汉初年,迟至1884年才出现零星英语节译;及至1995年美国学者约翰·梅杰正式领衔组建翻译团队,着手翻译这部博大精深的典籍,前后历时十五载,于2010年出版英语世界的第一部全译本,于2012年出版其精华版。全译本在提供相对准确可信译文的同时,还为有效阅读提供了译本序言、篇章导言、书后附录及索引等———这种“精准全译+充分配备”的译本模式对传统典籍翻译与出版颇有启迪。     

反思电子政务发展:基于美国地方政府的调查

经过20多年的建设,世界范围内的电子政务均得到不同程度的发展。为了验证早期电子政务发展阶段的理论,明确当前电子政务发展所处的阶段,反思新时期电子政务带来的变化及电子政务发展的主要受限因素,考察美国地方电子政务发展的轨迹,发现美国地方电子政务仍处于单向-双向沟通阶段的过渡期,电子政务发展所引发的变化导致地方政府的采纳行为有所保留。     

USP11通过去泛素化p53调控p53稳定性

研究目的：深入研究p53的泛素化及稳定性的调控。创新要点：发现一个新的调控p53去泛素化的酶USPll,它可以通过与p53的结合去泛素化并稳定p53,从而揭示了一个新的p53去泛素化调控的机制。研究方法：通过免疫共沉淀发现p53可以与USP11结合（图1a）,通过泛素化检测试验发现USPI1可以去泛素化p53（图3a和3b）,最后通过逆转录．聚合酶链式反应（RT—PCR）试验发现在DNA损伤后,USP11对p53转录活性的提高是非常重要的。     

美国高层次应用型国际商学教育研究——兼议中国现状与改革建议简

以美国四所商学院为研究样本,通过培养目标、培养内容、培养方式和国际商务人才知识技能结构的研究,对中国现状进行反思,提出了基于分层设定的思路实现培养目标的多元化和基于多维思路培养应用复合型人才的建议,以期对我国高层次应用型国际商务人才培养有所裨益。     

柑橘黄龙病菌外膜蛋白的诱导表达

柑橘黄龙病病菌的外膜蛋白(omp)极易被宿主免疫系统识别为外来物质,所以其被用制备抗体来检测柑橘黄龙病菌.本实验中截取了omp基因上的一段长为1 071 bp的片段,将其连接到p GEX6P1载体中制成了重组质粒omps-6p1进行原核诱导表达.结果表明,重组质粒可成功诱导出目的蛋白.     

“且看石壁犹歌舞,反弹琵琶慰柔肠”———浅谈《反弹琵琶品》中人物形象的塑造

敦煌壁画凝聚了整个敦煌文化艺术最精华的部分.一座座沉睡千年的石窟里,那千姿百态的“菩萨”“仙人”不仅仅是宗教艺术的遗存,也为我们刻画各个时代的舞蹈风貌提供了宝贵的舞蹈形象资料.本文试通过对敦煌舞剧目《反弹琵琶品》的表演实践,以演员创造角色过程的视角,分三个层次分析、总结其人物形象的塑造.     

Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle

The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen in liquid nitrogen immediately after collection and stored at −80 °C for RNA extraction, and the other was fixed in 40 mg/ml paraformaldehyde at room temperature for immunolocalization of BMP2 protein. Real-time PCR analysis showed that the expression level of Bmp2 was significantly higher at proestrus than at estrus and metestrus (P<0.05). The relative abundance of Bmp4 exhibited significant fluctuations, but there were no statistically significant differences between the expression levels of Bmp2 and Bmp4 (P>0.05). The expression levels of Bmpr1a and Bmpr2 remained unchanged during estrous cycles. However, the level of Bmpr1b mRNA decreased significantly at estrus (P<0.05), increasing subsequently at metestrus. Furthermore, the level of Bmpr1b mRNA was significantly lower than those of Bmpr1a and Bmpr2 mRNA at the corresponding stages (P<0.05). All three receptor-regulated Smads (R-Smads) detected were differentially expressed in the mouse uterus and the expression levels of Smad1 and Smad5 were significantly higher than that of Smad8 (P<0.05). In addition, the expression level of Smad4 did not change substantially throughout the estrous cycle. Immunohistochemical experiments revealed that BMP2 protein was differentially expressed and localized mainly in the uterine luminal and glandular epithelial cells throughout the estrous cycle. In conclusion, our results provide information about the variation in the mRNA levels of Bmp2 and Bmp4 and related components of the BMP signaling pathway. The data provide quantitative and useful information about the roles of endometrial BMP proposed and demonstrated by others, such as the degradation and remodeling of the endometrium.     

毕节市2012年度科普统计分析

根据2012年毕节市科普人员、科普场地、科普经费、科普传媒、科普活动等5个方面科普工作统计的数据,对毕节市的科普现状进行分析,提出相应对策。     

基于MPLS的移动IPv6网络切换方案

通过分析原有的移动IPv6网络切换方案,提出了一种改进的切换方案——基于MPLS技术的多转交地址切换.即在原有MIPv6协议中的绑定更新消息中增加2个bit,实现多个转交地址的灵活使用.理论分析证明此方案能够降低移动IPv6网络的切换时延和丢包率.     

海工企业特种设备管理的方法和手段

海工企业特种设备种类繁多,针对特种设备管理流程方面存在的问题,分析了问题产生的原因,提出了树立科学管理理念、完善管理组织和管理制度、加强设备操作人员管理的解决方法与途径,并运用KPI指标进行效果检验.结果表明：科学的管理制度可以大大降低特种设备发生事故的风险,这种方法同样可以应用于其他拥有特种设备的企业.