Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

虫媒登革病毒的研究——Ⅱ.登革病毒非结构蛋白NS1的纯化、鉴定及其在临床检测应用

本文从纯化Den2 NS1,Mab入手,自制了Mab亲和层析系统,从感染Den2的细胞膜中,分离提取了天然的Den 2 NS1双体,比较其NGC株与我国地方株(H-87)NS1的理化性质及组成,并对某些重要的生物学性质进行了综合性研究。同时,利用上述研究结果建立了检测DF病人血清特异NS1抗体的新方法,探索其协助临床诊断及研究的可能性。     

J2EE平台在行政审批服务系统中的应用

本文分析了电子政务对国民经济所起的重大作用,简要介绍了当前流行的J2EE架构的分布式对象结构特点。设计了一个J2EE架构下的行政审批服务系统,它由客户层、中间层和企业信息系统层等三个层次组成。同时,对系统的设计方法、软件结构及实现技术进行了探讨。     

自聚焦克尔类电介质中非傍轴光束调制非稳的研究

Propagation stability of non-paraxial beam in nonlinear Kerr media is investigated with a linear stability method.Both theoretical analysis and numerical simulation show that modulation instability(MI) gain spectrum has three different distribution features determined by the times of incident power P0 and the non-paraxial parameter a. Furthermore,the corresponding criterion is put forward to distinguish the three different distributions.     

Thermodynamic Analysis and Comparison on Low Temperature CO_2-NH_3 Cascade Refrigeration Cycle

Intheapplicationsofindustrialandcommercialrefrig erationsystem ,iftherequiredrefrigerationtemperatureisverylow ,theconventionalvaporcompressionsystemisdiffi culttomeettherequirementbecausewhenthedifferencebe tweentheevaporatingtemperatureandthecondensingtemper atureissohighthatitishardtofindarefrigeranttomeettheneedsasthecondensingpressureisnottoohighandtheevap oratingpressureisnottoolow .Whentherefrigeratingtemper atureisverylow ,thecascaderefrigerationcycleissuitabletobeused .Itcannotonlysat…     

关于粘性导数的几个性质的探讨

M .G .Crandall等人在文 [1]中给出了函数的粘性导数的概念。在这个概念的基础上我们讨论了函数的粘性导数的上 (下 )导集的几个性质 :在函数可微点处的性质、闭凸性质、与C1类函数的和的性质、与C2 类函数的差在严格局部极大 (小 )值点的性质。     

拒绝服务攻击原理与防御策略

介绍了拒绝服务攻击实施的原理，以及相关协议的漏洞和拒绝服务攻击的最新进展，并说明了如何结合防火墙和入侵检测系统等手段来防范其攻击。     

论21世纪图书馆的发展模式与功能

本文分析了影响决定图书馆发展模式的四个重要因素。即技术、资金、文化心态和制度变迁。认为模式的不同导致图书馆功能在不同时代的差异，并提出了21世纪图书馆的四种发展模式，阐述了它们的主要功能。     

一类超二次Hamilton系统的周期解的存在性

运用临界点理论中的极小、极大方法得到一类超二次哈密顿系统的周期解的存在性的存在性定理.     

Physical design method of MPSoC

Floorplan, clock network and power plan are crucial steps in deep sub-micron system-on-chip design. A novel di- agonal floorplan is integrated to enhance the data sharing between different cores in system-on-chip. Custom clock network con- taining hand-adjusted buffers and variable routing rules is constructed to realize balanced synchronization. Effective power plan considering both IR drop and electromigration achieves high utilization and maintains power integrity in our MediaSoC. Using such methods, deep sub-micron design challenges are managed under a fast prototyping methodology, which greatly shortens the design cycle.