高分辨质谱-互联网-地理信息系统三元融合技术绘制中国农药残留地图

如何获得海量农药残留检测结果数据并将其直观、实时地展现在地图上,是当前大数据时代背景下农药残留分析领域的重要研究内容。经过10多年的深入研究,在建立气相色谱-四极杆-飞行时间质谱(GC-Q-TOF/MS)485种和液相色谱-四极杆-飞行时间质谱(LC-Q-TOF/MS)525种农药精确质谱库的基础上,研究开发了非靶向、高通量GC-Q-TOF/MS和LC-Q-TOF/MS联用农药残留检测技术,可适用于1 200种残留农药检测。这使得农药残留检测效率得到了飞跃性的提高,为获得准确可靠的海量农药残留检测结果奠定了基础。文章将海量农药残留检测结果数据与互联网和地理信息技术相结合,研究建立了农药残留可视化系统,该系统由两部分构成:(1)在线制图系统。与Web-GIS技术相结合,并应用数据统计分析方法,创新性地以专题地图的形式,综合使用形象直观的地图、统计图表、报表等表达方式,多形式、多视角、多层次地呈现我国农药残留现状;(2)纸质地图。采用系统的思想集成表达了农药残留的空间分布、农药种类、农产品类型、残留量、毒性、超标情况等多种维度的信息。目前,农药残留可视化系统已经应用于2012—2015年采集于全国31省会/直辖市284县区638个采样点的22 278批水果和蔬菜样品的高分辨质谱农药残留筛查结果中。结果表明,基于三元融合技术构建的农药残留可视化系统,可以直观、实时展现海量农药残留数据,实现了农药残留结果的地图可视化,并将成为我国食品安全风险监控的强有力的工具。这项研究将为"十三五"国家战略发展规划中提出的实现"农药零增长"和"推进健康中国建设"提供技术和科学数据的支撑。     

阴香叶挥发性物质的GC／MS分析

利用顶空固相微萃取技术（SPME）吸附采集阴香叶的挥发性物质,采用气相色谱-质谱（GC／MS）技术对其进行分析研究．结果显示,从阴香叶中鉴定出39种化学物质,占挥发性物质总量的98．48％,其主要挥发性物质为龙脑（19．68％）、桉树脑（10．49％）、乙酸双环【2．2．1】庚-2-基酯（9．65％）、α-蒎烯（7．36％）、D-柠檬烯（6．45％）、石竹烯（5．80％）等,这些物质的共同作用形成了阴香叶特殊的香味．     

Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L

The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride (CCl₄)-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels caused by CCl₄ intoxication. It also significantly prevented the decrease in superoxide dismutase (SOD) activity and the increase in malondialdehyde (MDA) content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL.     

教育投入对经济增长的影响恒久不变吗--改革开放以来的路径演化分析

本文基于改革开放以来我国国家财政预算内教育经费以及 GDP 年度数据,运用非线性 MS (M)-VAR(p)模型,对我国教育投入与宏观经济增长之间的内在联动机制进行测度。结果表明：(1)教育投入增长率在较低水平时波动性较小、持续性较大,在较高水平时波动性较大、持续性较小。(2)宏观经济处于“低速”或“快速增长”态势时波动性较大,持续性较小;处于“中速增长”阶段时,波动性较小、持续性较大。(3)前期的 GDP 增长率对当期的教育投入增长率具有正向影响,前期的教育投入增长率对当期的GDP 增长率具有负向影响。(4)“教育与经济”系统处于“低速”或“中速增长区制”时,可能性更大、持续性更强,教育投入增长率与GDP增长率之间负相关;处于“快速增长区制”时,可能性最小、持续性最弱,教育投入增长率与GDP增长率之间正相关。     

Method Development and Validation of Liquid Chromatography-Tandem Mass Spectrometry for Angiotensin-II in Human Plasma: Application to Study Interaction Between Atorvastatin & Olmesartan Drug Combination

Simple and sensitive Liquid Chromatography-Tandem Mass Spectrometry (LCMS/MS) method was developed and validated, then was implicated on hypertensive human subjects to study drug interaction of atorvastatin (ATVS) and Olmesartan (OLM) on status of Angiotensin-II (ANG-II). The ANG-II in plasma was extracted with 5 mL methanol containing 5 % formic acid through C₁₈ (cartridges) liquid–liquid extraction, dried and reconstituted with 1 mL of 16 % acetonitrile in 0.1 % formic acid in water. The chromatographic separation of ANG-II with a Agilent technology 6410 Triple quadrupole was carried multiple reaction monitoring scan mode with a Agilent 1290 Infinity LC system for UHPLC. The sample were separated on a (Thermo Scientific) Hy-Purity advance (50 × 4.6 mm, 5 μm) using Mobile Phase A: 16 % acetonitrile in 0.1 % formic acid in water and Mobile Phase B: 0.1 % formic acid in methanol at a flow rate of 0.3 mL/min, performed at ambient temperature. The mobile phase gradient of 16 % acetonitrile in water was linearly increased to 38 % acetonitrile over 10 min and subsequently the mobile-phase was increased to 100 % acetonitrile over 15 min. The developed method was validated for specificity, accuracy, precision, stability, linearity, sensitivity and recovery. The method was linear between peak area ratio of standard and internal standard over the range of 50–800 ng/mL. The method was successfully applied for the drug interaction study revealed levels of ANG-II were significantly higher in ATVS + OLM treatment condition as compared to individual treatment of OLM. This reflects the reason of low effectiveness of ATVS + OLM in combination instead of synergistic activity.

Electronic supplementary material

The online version of this article (doi:10.1007/s12291-014-0457-x) contains supplementary material, which is available to authorized users.     

关于IMS中基于SIP协议的应用分析

IMS(IP Multimedia Subsystem)是IP多媒体子系统,是一种全新的多媒体3G业务形式,它能够满足现在的终端客户更新颖、更多样化多媒体业务的需求。目前,IMS被认为是下一代网络的核心技术,也是解决移动与固网融合的重要方式。它用基于SIP信令的IP网络对多媒体传输业务实现了可靠多元的控制。SIP(Session Initiation Protocol)是一项类似于HTTP的基于文本的C/S模式点对点通信协议,用于建立、修改和终止IP多媒体对话,为电信运营商及其合作伙伴和用户提供了新一代基于IP的服务。     

长裂苦苣菜挥发油成分的GC/MS分析

目的:鉴定长裂苦苣菜挥发油的化学成分。方法:用水蒸气蒸馏法提取长裂苦苣菜挥发油,采用气相色谱-质谱联用仪分析和保留指数分析相结合的方法,鉴定其挥发油的化学成分。结果:共鉴定出36种成分,所鉴定的组分占挥发油总离子流色谱峰面积的89.49%。结论:长裂苦苣菜挥发油的主要成分为6,10,14-三甲基-十五烷-2-酮(35.8%)、十六烷酸(35.3%)。     

玉桂茶泡剂中稀土元素的电感耦合等离子体质谱分析

建立了一种电感耦合等离子体质谱法测定玉桂茶泡剂中稀土元素的方法.在优化的条件下,15种稀土元素的检出限在0.00004～0.003 ng/mL范围,相对标准偏差在0.6%～2.6%范围.利用该方法分析了国家一级茶叶标准物质(GBW070605),结果与参考值一致.该方法被用于分析玉桂茶样品中的稀土元素,得到令人满意的结果.