重瓣黄刺玫休眠芽快繁技术的研究

探讨重瓣黄刺玫组培快繁的实用技术,筛选出重瓣黄刺玫的休眠芽培养的最佳培养基配方及激素水平(2/3MS-MAA1.3mg/L+BA3mg/L),阐明了重瓣黄刺玫的休眠芽培养、继代培养及生根培养技术和移栽成活、壮苗培育的科学栽培技术,找到了一条快速繁殖黄刺玫的新途径.     

UPLC-MS／MS法测定口服银杏叶片的大鼠血浆中七种活性成分及药代动力学研究

研究目的：建立一个快速灵敏且具有专属性的体内分析方法用于银杏叶片的药代动力学研究。 创新要点：运用超高压液相色谱-二级质谱连用法（UPLC-MS／MS）进行大鼠血浆中七种活性成分的测定,方法简单快速,适合于银杏叶制剂的药代动力研究。 研究方法：采用液液萃取进行血浆样品处理,该方法简单且快速。采用超高压液相色谱（UPLC）进行七种活性成分分离,达到了多种成分快速分离的目的。采用二级质谱（MS／MS）进行检测,优化质谱条件,减小了内源性干扰并提高检测灵敏度。经过专属性、线性、回收率、基质效应、精密度、准确度和稳定性考察,验证了该方法的适用性。经验证,该方法适用于银杏叶片的药代动力学研究。 重要结论：液液萃取处理血浆样品简单可靠,回收率稳定,无基质效应（表4）。UPLC-MS／MS能够快速、灵敏、专属性地分析大鼠血浆七种活性成分的血药浓度（表3和图3）。该方法适用于银杏叶片的七种活性成分的药代动力学研究（图4和5）。     

伴发ATTR型淀粉样沉积的骨关节炎患者的临床和生物学特征分析(英文)简

Objective： Our aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints. Methods： Synovial membranes were obtained from 36 patients with knee osteoarthritis （OA） who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was deter- mined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All syn- ovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain （AL-K and AL-,k）, serum amyloid-A （SAA）, amyloidogenic transthyretin （ATTR）, and amyloidogenic 152- microglobulin （A152M）. Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry （MALDI-TOF MS） was used to analyze transthyretin （TTR） isoforms in the serum of each patient. Results： Nine cases （25%） were found to be amyloid-positive. Immunohistochemicaliy, eight cases （88.9%） had ATTR deposition, and one sample （11.1%） was shown to be AL-K-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were sig- nificantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group. Conclusions： Amyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-K. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints.     

Anterolateral minithoracotomy versus median sternotomy for mitral valve disease: a meta-analysis

Objective

Mitral valve disease tends to be treated with anterolateral minithoracotomy (ALMT) rather than median sternotomy (MS), as ALMT uses progressively smaller incisions to promote better cosmetic outcomes. This meta-analysis quantifies the effects of ALMT on surgical parameters and post-operative outcomes compared with MS.

Methods

One randomized controlled study and four case-control studies, published in English from January 1996 to January 2013, were identified and evaluated.

Results

ALMT showed a significantly longer cardiopulmonary bypass time (P=0.001) and aortic cross-clamp time (P=0.05) compared with MS. However, the benefits of ALMT were evident as demonstrated by a shorter length of hospital stay (P<0.00001). According to operative complications, the onset of new arrhythmias following ALMT decreased significantly as compared with MS (P=0.05); however, the incidence of peri-operative mortality (P=0.62), re-operation for bleeding (P=0.37), neurologic events (P=0.77), myocardial infarction (P=0.84), gastrointestinal complications (P=0.89), and renal insufficiency (P=0.67) were similar to these of MS. Long-term follow-up data were also examined, and revealed equivalent survival and freedom from mitral valve events.

Conclusions

Current clinical data suggest that ALMT is a safe and effective alternative to the conventional approach and is associated with better short-term outcomes and a trend towards longer survival.     

绿色荧光蛋白的原核表达和纯化及鉴定的实验设计与实践

该文以绿色荧光蛋白FRP为实验对象,通过设计原核蛋白表达、镍柱亲和纯化、荧光光谱分析、SDSPAGE电泳分离、Western blotting免疫印迹及蛋白质质谱鉴定等内容的生物化学综合实验,提高学生的生物化学实验技能。荧光光谱分析表明,纯化的GFP蛋白的最大激发波长为480nm,最大发射波长为500nm,与已知的GFP荧光光谱基本相同。SDS-PAGE电泳分离发现,GFP的亲和纯化效果好,且纯化得到的GFP分子量约为27kDa,与理论值相符。该实验适用于生物科学专业本科生系统完整地学习蛋白质表达、纯化和鉴定的实验方法,具有很好的综合性,取得非常好的教学效果。     

GC/MS研究白兰叶挥发性物质释放动态

利用顶空固相微萃取技术（SPME）吸附采集白兰叶的挥发性成分,采用GC/MS技术分析鉴定,并用峰面积归一化法定量分析.测定出白兰叶在一年内不同生长阶段释放的挥发性物质,其中主要包括醇类、萜类、酯类和烷烃四大类化合物,以醇类和萜类为主.在不同生长阶段,挥发性物质的释放量不同,以8月份最大.     

白茶挥发性成分的固相微萃取-气相色谱-质谱分析

报道了用固相微萃取法提取白茶中挥发性化学成分,用气相色谱-质谱法从白茶挥发油中分离并确定出36种化学成分,其中主要成分为苯甲醛（5.91%）、苯乙醇（5.46%）、芳樟醇（9.02%）.     

汽车零部件企业生产管理系统设计

在全球信息化背景下,制造业的信息化应用水平,代表着企业的竞争力。生产管理系统是制造业信息化的核心系统之一。十堰市作为全国最大的汽车工业基地,拥有全国最为集中的汽车零部件制造企业,针对汽车零部件企业的独特性,量身定制生产管理系统,提升企业的管理水平,对企业及地方发展有着重要意义。     

固相萃取-UPLC—MS／MS测定食品中的黄曲霉毒素M1和B1

建立固相萃取与UPLC—MS／MS检测食品中黄曲霉毒素M1和黄曲霉毒素B1的方法。样品经乙腈提取和SPE小柱萃取净化,提取液经氮气吹干后,用50％甲醇水溶液定容。超高效液相色谱一串联质谱（UPLC—MS／MS）测定,采用电喷雾正离子（ESI＋）模式电离,多反应监测（MRM）模式检测。流动相为0．1％甲酸水溶液和甲醇,流速0．4ml／min,使用ZORBAX Eclipse XDB—C18色谱柱（100mm×3．0mm×1．8μm）为分析柱。黄曲霉毒素AFM1在0．1～50μg／kg范围内线性关系良好、黄曲霉毒素AFB1在0．1～10ug／kg范围内线性关系良好,相关系数均大于0．999;回收率在83．75％～97．8％之间;检出限AFMl为0．05μg／kg、AFBl0．02μg／kg。该法具有分析速度快,检测准确、灵敏度和回收率高等优点,适合食品中黄曲霉毒素M1、B1含量的检测。