纤维素酶研究现状及其在畜牧业中的应用

本文综述了纤维素酶的分子生物学、分子结构、作用机理、发酵生产以及纤维素酶在畜牧业中的应用现状。     

Study on the distribution of active centers in novel low Ti-loading MgCl2-supported Ziegler-Natta catalyst

Novel MgCl2-supported Ziegler-Natta (Z-N) catalysts prepared using a new one-pot ball milling method caneffectively control the amounts of Ti-loading in the catalysts. Complex GPC data on polypropylene synthesized by thesenovel catalysts were analyzed using the method of fitting the molecular weight distribution (MWD) curves with a multipleFlory-Schulz function. It was found that multiple active centers exist in these novel catalysts. Detailed study of the effects ofthe Ti-loadings in the catalysts on the distribution of the active centers showed that the Ti-loadings in the novelMgCl2-supported Z-N catalysts might affect the proportion of each type of active centers; and might be the main factorresponsible for the effect of the Ti-loadings on the microstructure, the molecular weight and molecular weight distributionwidth of the resultant polymer, the catalytic activity and polymerization kinetics.     

运用AP-PCR对中国慈姑属内亲缘关系的研究

用AP-PCR技术测定了中国慈姑属植物基因组DNA的多态性,以其为分子性状用UPGMA方 法聚类,结果表明:中国慈姑属植物可分为3大类,包括7种3变种或变型(未包括浮叶慈姑)。本文的结果与基于形态和核型分析的结果一致。     

具有势函数V(r)=A1r-10-A2r-6+A3r2分子晶体的Hamiltonian本征方程的精确解

本文采用连分法得到描述分子晶体中分子之间的相互作用势V(r)=Atr-10-A2r-6+A3r2(A1,A2,A3＞0)的分子晶体的Hamiltonian算子的精确的能量本征值和能量本征函数.     

Advances in the phylogenesis of Agaricales and its higher ranks and strategies for establishing phylogenetic hypotheses

We present an overview of previous research results on the molecular phylogenetic analyses in Agaricales and its higher ranks (Agaricomycetes/Agaricomycotina/Basidiomycota) along with the most recent treatments of taxonomic systems in these taxa. Establishing phylogenetic hypotheses using DNA sequences, from which an understanding of the natural evolutionary relationships amongst clades may be derived, requires a robust dataset. It has been recognized that single-gene phylogenies may not truly represent organismal phylogenies, but the concordant phylogenetic genealogies from multiple-gene datasets can resolve this problem. The genes commonly used in mushroom phylogenetic research are summarized.     

"分子生物学实验"课程体系的建立和考核方法的改革

本文在比较国内著名高校的分子生物学实验课程体系基础上,结合我校教学计划,将科研成果转化为实验教学综合性实验项目,设置具有特色的实验课程体系。采用多媒体实验教学方法,增加设计性实验项目,突出“研究型特色”。此外,在期末考试中,学生须将实验报告整理成课程论文。     

二化螟隐花色素基因cryptochrome1和cryptochrome2的克隆与表达谱分析

隐花色素基因(cryptochrome,简称cry)是一类生物钟基因,参与生物体昼夜节律调控。本实验克隆了二化螟2个隐花色素基因,分别命名为Cs-cry1和Cs-cry2(Genbank登录号分别为HG780135和KF977409),其分别包含1605 bp和2289 bp的开放阅读框,分别编码由534和762个氨基酸组成的蛋白;Cs-cry1与其它鳞翅目昆虫cry1的相似性较高,而Cs-cry2与其它鳞翅目昆虫cry2的相似性较高,这与系统进化分析结果相一致;半定量RT-PCR研究表明Cs-cry1和Cs-cry2基因在二化螟成虫触角、头、雄、腹、足和翅等不同组织中均有表达。实验结果为二化螟昼夜节律分子机制研究提供了理论依据。     

分子生物学主体性教学模式的探索

分子生物学是生命科学基础专业课,根据高职高专课程体系改革的基本指导思想,结合郧阳师专办学特色和生物教育专业的特点,探索该课程的教学方法、教学内容,考核方法等.通过实施优化课程设置、重组课程体系、因材施教、采用多媒体教学手段和实施教学等一系列主体教学措施,构建一套适合高职高专生物专业发展的分子生物学主体教学模式.     

Clones identification of Sequoia sempervirens (D.Don) Endl.in Chile by using PCR-RAPDs technique

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin.     

研究生细胞与分子免疫学课程的改革探索

课程改革是培养研究生创新能力,提高教学质量的重要途径。本文从课程教学内容、教学模式和教学资源等方面入手,对研究生细胞与分子免疫学课程进行改革和实践,取得了很好的教学效果,实现了知识传承与知识创新的融合,知识的学习与学生素质、能力的培养同步,对于提高研究生教学质量有着借鉴意义。