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31.
Manuel TORAL IBAEZ Margarita CARU Miguel A.HERRERA Luis GONZALEZ Luis M.MARTIN Jorge MIRANDA Rafael M.NAVARRO-CERRILLO 《Journal of Zhejiang University. Science. B》2009,10(2)
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin. 相似文献
32.
Manuel Toral Ibañez Margarita Caru Miguel A. Herrera Luis Gonzalez Luis M. Martin Jorge Miranda Rafael M. Navarro-Cerrillo 《Journal of Zhejiang University. Science. B》2009,10(2):112-119
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene
diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied.
The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.
The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis
in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and
were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index
and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).
Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of
49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic
variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a
geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although
they are derived from the same geographic origin.
Project (No. CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile 相似文献
33.
植物分子生物学和植物生物技术利用分子标记技术得到了大力发展,分子标记的创新、发展与应用对其起了积极的推动作用,本文就RFLP、AFLP、RAPD、DAF、SSR、CAPS分子标记的原理与技术及其在甘薯种质资源和遗传育种中的应用作一介绍 相似文献
34.
周国中 《金华职业技术学院学报》2003,3(2):39-41,61
本文采用连分法得到描述分子晶体中分子之间的相互作用势V(r)=Atr-10-A2r-6+A3r2(A1,A2,A3>0)的分子晶体的Hamiltonian算子的精确的能量本征值和能量本征函数. 相似文献
35.
课程改革是培养研究生创新能力,提高教学质量的重要途径。本文从课程教学内容、教学模式和教学资源等方面入手,对研究生细胞与分子免疫学课程进行改革和实践,取得了很好的教学效果,实现了知识传承与知识创新的融合,知识的学习与学生素质、能力的培养同步,对于提高研究生教学质量有着借鉴意义。 相似文献
36.
实验教学是培养大学生创新能力的重要途径和手段,但是传统实验教学模式存在较多问题,不能满足培养高素质医学人才的要求。"生物化学与分子生物学"是临床医学专业的基础课,而实验教学是该课程教学内容的一部分,是培养医学生创新能力的重要途径和手段。以"生物化学与分子生物学"实验教学为例,从优化实验教学设计、丰富实验教学内容、改革考核方式等方面进行实践和总结,阐述如何在实验教学中培养学生创新思维,提升实验教学质量,为实验教学改革提供新的思路和参考。 相似文献
37.
38.
Hua Yu Mao-Jun Wang Nan-Xia Xuan Zhi-Cai Shang Jun Wu 《Journal of Zhejiang University. Science. B》2015,16(10):883-896
Objective: To provide essential information for peptide inhibitor design, the interactions of Eps15 homology domain of Eps15 homology domain-containing protein 1 (EHD1 EH domain) with three peptides containing NPF (asparagine-proline-phenylalanine), DPF (aspartic acid-proline-phenylalanine), and GPF (glycine-proline-phenylalanine) motifs were deciphered at the atomic level. The binding affinities and the underlying structure basis were investigated. Methods: Molecular dynamics (MD) simulations were performed on EHD1 EH domain/peptide complexes for 60 ns using the GROMACS package. The binding free energies were calculated and decomposed by molecular mechanics/generalized Born surface area (MM/GBSA) method using the AMBER package. The alanine scanning was performed to evaluate the binding hot spot residues using FoldX software. Results: The different binding affinities for the three peptides were affected dominantly by van der Waals interactions. Intermolecular hydrogen bonds provide the structural basis of contributions of van der Waals interactions of the flanking residues to the binding. Conclusions: van der Waals interactions should be the main consideration when we design peptide inhibitors of EHD1 EH domain with high affinities. The ability to form intermolecular hydrogen bonds with protein residues can be used as the factor for choosing the flanking residues. 相似文献
39.
测定了红豆杉科、三尖杉科、罗汉松科及松科共9属13种植物的matK基因序列,发现3′端的单碱基插入造成mat k基因在这些类群间有较大的长度变异(1488∽1548bp),其它插入或缺失的长度为3、6或9bp(白豆杉中有一个长为27bp的缺失),且绝大多数插入或缺失事件具有系统发育信息。matK基因中,密码子第1、2及3位的变异率较为相近,平均同义替代率约为平均非同义替代率的2倍。以松科2属3种植物为外类群,运用PAUP软件进行的系统发育分析仅得到一棵最简约树(步长为895,CI=0.850,RI=0.876),该简约树的各分支均得到bootstrap分析的极强支持。结果表明:红豆杉科与三尖杉科均为单系群,二者互为姐妹群;白豆杉属与穗花杉属为红豆杉科中两个很自然的属,白豆杉属为红豆杉属的姐妹群,穗花杉属为榧树属的姐妹群。竹柏属与罗汉松属聚为一支,二者间的亲缘关系得到boot-strap分析的100%支持,从分支分类学及遗传距离角度,作者不赞同将竹柏属提升为科。 相似文献
40.