珍稀濒危植物珙桐幼苗光合特征研究

为了研究珙桐叶片光合特征及其与环境因子的相互关系,对珙桐的引种驯化及资源保护提供重要的理论依据,采用美国产Li-6400型便携式光合测定系统,测定了珙桐叶片的光合作用日变化以及光响应曲线。结果表明：珙桐叶片的表观光量子速率0.09μmol/mol,暗呼吸速率1.31μmol.m-2.s-1,光补偿点22.01μmol.m-2.s-1,光饱和点465.18μmol.m-2.s-1;珙桐叶片的净光合速率、蒸腾速率和气孔导度的日变化曲线均呈双峰曲线,存在明显的＂午休＂现象;珙桐叶片净光合速率与气孔导度、蒸腾速率呈极显著正相关;气孔导度和蒸腾速率是影响珙桐光合速率的生理因子。     

Characterization of high-yield performance as affected by genotype and environment in rice

We characterized yield-relevant characters and their variations over genotypes and environments (locations and years) by examining two rice varieties (9746 and Jinfeng) with high yield potential.9746 and Jinfeng were planted in two locations of Shanghai,China,during 2005 and 2006.The results show that there was a large variation in grain yield between locations and years.The realization of high yield potential for the two types of rice was closely related to the improved sink size,such as more panicles per square meter or grains per panicle.Stem and leaf biomasses were mainly accumulated from tillering stage to heading stage,and showed slow decline during grain filling.Meanwhile,some photosynthetic characters including net photosynthesis rate (Pn),leaf area index (LAI),specific leaf area (SLA),fluorescence parameter (maximum quantum yield of PSII,Fv/Fm),chlorophyll content (expressed as SPAD value),as well as nutrient (N,P,K) uptake were also measured to determine their variations over genotypes and environments and their relationships with grain yield.Although there were significant differences between years or locations for most measurements,SLA at tillering and heading stages,Fv/Fm and LAI at heading stage,stem biomass at heading and maturity stages,and leaf nitrogen concentration at tillering and heading stages remained little changed,indicating their pos-sible applications as selectable characters in breeding programs.It was also found that stem nitrogen accumulation at tillering stage is one of the most important and stable traits for high yield formation.     

Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction inRubsico activase and/or the reduction in carbamylation.     

Effect of terminal locations of pods on biomass production and 13C partitioning in a fasciated stem soybean Shakujo

This study addresses the hypothesis that stagnation of soybean yield on the farm can be improved by selection of a physiological trait favoring carbon assimilate partitioning to terminally placed pods versus genotypes having axillary pods at close plant spacing. ¹³C was fed to source-sink units comprising a leaf, axillary/terminal pods, and petioles at upper and lower positions of the stem axis in two soybean cultivars, namely Shakujo and Enrei, at different densities of populations. The cultivars differ significantly in architecture, Shakujo bearing a few hundreds of pods in close succession to one another in a terminally placed raceme, in contrast to Enrei having axillary racemes. Pod yield per plant was higher in Enrei than in Shakujo at low density, but Shakujo out-yielded Enrei at close spacing. Population density decreased yield per plant and altered the pattern of assimilate partitioning significantly within the plants for both varieties. At high density more assimilates moved to the upper parts at the cost of the lower parts. The terminally placed pods of Shakujo were advantaged to receive assimilates under density stress. No benefit was accrued to pod filling of Enrei, however, under this condition.     

Interactions of cadmium and aluminum toxicity in their effect on growth and physiological parameters in soybean

The effect of Al and Cd on the growth, photosynthesis, and accumulation of Al, Cd and plant nutrients in two soybean genotypes were determined using hydroponic culture. There were six treatments: pH 6.5; pH 4.0; pH 6.5 1.0 μmol/L Cd; pH 4.0 1.0 μmol/L Cd; pH 4.0 150 μmol/L Al; pH 4.0 1.0 μmol/L Cd 150 μmol/L Al. The low pH (4.0) and Al treatments caused marked reduction in root length, shoot height, dry weight, chlorophyll content (SPAD value) and photosynthetic rate. Al-sensitive cv. Zhechun 2 accumulated comparatively more Al and Cd in plants than Al-tolerant cv. Liao 1. Compared with pH 6.5, pH 4.0 resulted in significant increase in Cd and Al concentration in plants. Combined application of Cd and Al enhanced their accu-mulation in roots, but caused a reduction in shoots. The concentrations of all 10 nutrients (P, K, Ca, Mg, Fe, Mn, Cu, Zn and B), except Mo were also increased when plants were exposed to pH lower than pH 6.5. Al addition caused a reduction in the con-centration of most nutrients in plant roots and shoots; but K, Mn and Zn in roots were increased. Treatments with Cd alone or together with Al reduced the concentrations of all the plant nutrients in plants. Al-sensitive genotype Zhechun 2 has lower nutrient concentration than Al-tolerant genotype Liao 1. The current findings imply that Al and Cd are synergistic in their effect on plant growth, physiological traits and nutrient uptake.     

苔藓植物光合作用荧光光谱和动力学荧光的比较

本文首次比较了光合作用的荧光光谱和荧光动力学在苔藓植物的原始种类和进化种类之间的异同。原始的和进化的苔藓植物具有发射波长相同的室温荧光光谱，其发射高峰位于686-690nm(来自光系统Ⅱ)和736-740nm(来自光系统Ⅰ)。  而它们的低温(77K)荧光光谱有三个发射峰：F687-689和F697-699来自光系统Ⅱ，F723-734来自光系统I。 前两个峰在原始的和进化的种类中基本相同。  按第三个发射峰可把被测的苔藓植物分为两组：发射峰在725nm左右的有细牛毛藓、长肋对齿藓、对齿藓、斜叶芦荟藓、密集匍灯藓和地钱，它们是较原始的藓类和较进化的苔类；发射峰在732nm左右的有细枝羽藓、东亚金灰藓、鼠尾藓、鳞叶藓、粗枝藓和美灰藓等较进化的藓类，也有较原始的钝叶匍灯藓。已知光系统I核心复合物CPI的77K荧光发射峰在722nm，而CPIa(核心复合物与外周天线复合物)和LHC-I(外周天线复合物)的发射峰在730nm。这说明在苔藓植物进化过程中，光系统Ⅱ比较保守；而光系统Ⅰ有所变化，原始的藓类主要含有光系统Ⅰ核心复合物，而较进化的藓类才含有较完善的外周天线复合物。光合作用荧光动力学分析表明，在原始藓类和地钱中具有较低的光系统Ⅱ活性、光系统Ⅱ的原初光能转换效率、光合碳同化和潜在的光合量子转换效率；而较进化的具有较高的活性和效率。 但是，原始的密集匍灯藓也具有较高的活性和效率，而进化的美灰藓却具有较低的活性和效率。这可能表明这两种植物是由原始向进化发展过程中的中间类型。     

高温细雾喷水对植物光合作用的影响

根据植物光合作用推算,作物的光能利用率应达到5%左右,而现在即使高产田光能利用率只达到3%左右,产量升高的潜力还很大,制约作物产量的原因有很多,本研究通过对植物进行高温下细雾喷水,一为证明过度蒸腾和过强光照是目前制约产量的原因之一;二为找出一条除一般的生产措施外,提高作物产量的新路子来。本课题研究的方法对产量的提高是绿色的无污染的,是作物可持续生产的一条新途径。     

Synergistic interaction of NaCl and Cd on growth and photosynthetic parameters in soybean genotypes differing in salinity tolerance

The effects of salinity(50 mmol/L NaCl) and Cd(1 μmol/L CdCl2) as sole and combined on growth and photosyn-thetic parameters were studied using two soybean genotypes,Huachun 18 and NGB.The concentrations of Cd2 ,Zn2 ,Ca2 ,Mg2 ,K and Na were also determined in seeds and pods.Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate(Pn) in the treatments of salinity stress alone and combined stress(NaCl Cd),showing that it is relatively sensitive to salinity.The decrease in Pn caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency(the ratio of variable fluorescence to maximal fluorescence,Fv/Fm),whereas the decease in NGB was mainly related to reduced stomatal conductance(Gs).The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone.Greater change in the pod concentrations of Zn2 ,Ca2 ,Mg2 ,K and Na was detected under salt stress for Huachun 18 than for NGB.The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes.     

红壤丘陵地区双季稻光合特性初步研究

亚热带红壤丘陵地区水稻叶片净光合速率与蒸腾速率在夏季晴天中午有降低现象，其日变化曲线呈双峰型。PAR和温度的日变化是形成光合速率日变化的主要环境因子。较高的温度会导致蒸腾强度降低，而水分亏缺引起的气孔导度降低气孔阻力增大则造成光合速率的降低。水稻上部叶片的光补偿点低于下部叶片，而光饱和点则是下部叶片低于上部。表观初始光能利用效率第2片叶为0.049-0.068，第4片叶为0.044-0.060。光能利用率第2片叶在2.6%-5.7%，第4片叶在2.0%-5.8%。水稻叶片的最大光合速率早稻和晚稻旗叶、2叶、4叶分别可达到19.58μmol CO2m-2、17.78μmol CO2m-2、14.4μmol CO2m-2 s-1和26.46和17.27μmol CO2m-2 s-1     

室内植物在现代家庭中的运用

本文通过介绍室内植物概念及起源,指出了现代家庭中运用室内植物的有利作用,并着重提出了不利的作用.