银针、艾灸治疗软组织损伤的疗效观察

通过采用银针加艾灸的方法,对106名运动员急慢性软组织损伤的治疗效果进行观察,发现疗效优良者占80.19％,中占16.98％,差占2.83％。由于银针的针体较粗,导热性能良好,针灸后可改善受伤部位微循环,具明显镇痛作用,疗效较好。     

Metachronous contralateral testicular and bilateral adrenal metastasis of chromophobe renal cell carcinoma: a case report and review of the literature

Chromophobe renal cell carcinoma(ChRCC) metastatic to the testis has not,to the best of our knowledge,been reported in the literature.Nor have there been reports of delayed bilateral adrenal metastasis of ChRCC.Here we report a case of metachronous contralateral testicular and bilateral adrenal metastasis of ChRCC in a 70-year-old man who underwent right radical nephrectomy for RCC six years ago.He was admitted to the hospital because of left intrascrotal enlargement of two-month duration.Ultrasonography re...     

川芎嗪治疗实验性肾损伤机理的研究

目的:观察川芎嗪治疗实验性免疫性肾损伤作用和机理,为中药治疗肾小球肾炎开辟新途径。方法:猪血清腹腔注射8周,复制大鼠实验性肾损伤模型,川芎嗪治疗4周后处死动物,检测尿蛋白、血清BUN和Scr含量,取肾组织制作光、电镜标本检查。结果:川芎嗪大、小剂量组尿蛋白、血清BUN和Scr含量较模型组明显降低(P<0.01),肾小球的病理形态学改变较模型组轻。结论:川芎嗪具有治疗大鼠实验性免疫性肾损伤的作用。     

Science Letters：Application of new tissue microarrayer-ZM-1 without recipient paraffin block

The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA （tissue microarray） paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method＇s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.     

微课在动物解剖与组织胚胎学教学改革中的应用

动物解剖与组织胚胎学是动物医学专业学生的核心基础课,该课程不仅具有多、广、杂等特点,而且较抽象,老师授课难度大,学生学起来感觉枯燥乏味。针对该课程授课中存在的问题,进一步优化教学大纲,对每个章节的重点内容,参考国内外名校的公开课视频,制作微课和微视频,利用QQ和微信交流,实行线上线下学习相结合,取得了一定成效。     

Lipid Parameters, Doses and Blood Levels of Calcineurin Inhibitors in Renal Transplant Patients

The calcineurin inhibitors (CNIs) [cyclosporin A (CsA) and tacrolimus (Tac)] are currently the most widely prescribed drugs for maintenance of immunosuppression after renal transplantation. These immunosuppressants are associated with side effects such as hyperlipidemia. We evaluated the differential effects of different CNIs on serum lipid parameters in renal transplant patients. Moreover, the aim of this study is to investigate the relationships between doses and blood levels of CNIs, and blood levels of CNIs and lipid parameters retrospectively. Two groups of 98 non-diabetic renal transplant patients, each treated with different CNIs, were studied: group A (n = 50, mean age: 31 ± 10 years), CsA, mycophenolate mofetil/azathioprin, steroid; group B (I = 48, mean age: 34 ± 12 years), Tac, mycophenolate mofetil/azathioprin, steroid. In renal transplant patients, CNIs blood levels and doses were examined at 1, 3, 6, 9, and 12 months after transplantation. Biochemical laboratory parameters including plasma lipids [total-cholesterol (CHOL), low-density lipoprotein (LDL)–CHOL, high-density lipoprotein (HDL)–CHOL, and triglycerides (TG)], CNI levels and doses were examined at 1, 3, 6, 9, and 12 months after transplantation. None of the patients received anti-lipidemic drugs during the study period. Blood levels of CNIs were detectable in all whole-blood samples by Cloned- Enzyme-Donor Immunoassay (CEDIA). The relationship between CNIs blood levels and CHOL, (LDL)–CHOL, HDL–CHOL, TG were evaluated. The mean serum CHOL levels and LDL–CHOL levels of patients in group A were found significantly higher than the patients in group B during the 12 month of follow up (p < 0.05). There was no significant difference in TG and HDL–CHOL plasma levels between group A and group B (p > 0.005). In group A the daily dose of CsA was significantly correlated with the mean blood levels of CsA at the 1st and 3rd months (r = 0.387, p = 0.005; r = 0.386, p = 0.006), respectively. In group A, the daily dose of CsA was significantly correlated with the mean serum TG levels during the 12 month of follow up (r = 0.420, p = 0.003). In group B, the daily dose of Tac was significantly correlated with the mean blood level of Tac (r = 0.335, p = 0.020) at the 1st month. No correlation was found between mean Tac blood levels and lipid parameters during the 12-month of follow up (p > 0.05). Significant positive correlation was observed between the CsA blood levels and LDL–CHOL levels (r = 0.338, p = 0.027) at the 3rd month. In the renal transplant patients with well functioning grafts, CsA therapy is associated with increased CHOL and LDL–CHOL ratio which represents an increased atherogenic risk tended to be associated with CsA. Serum LDL–CHOL levels may be effected by blood CsA levels.     

烟草组织培养的研究进展及应用前景

植物组织培养是生物工程应用基础研究的重要组成部份,是植物基因工程研究中的一项重要技术手段。本文就楚雄州实际出发打牢农业基础,继续巩固烤烟这个支柱;增加两烟的科技含量,加快培育新的支柱产业和新的经济增长步伐,初步分析了组织培养技术在农业烟草生产中的重要经济价值和巨大应用价值。     

Vitamin B<Subscript>12</Subscript> Immunoassay on Roche Elecsys 2010: Effects of High Excess Concentration of Serum Vitamin B<Subscript>12</Subscript> in CKD Patients on Parenteral Administration

Vitamin B₁₂ being water soluble is excreted in the urine when administered in excess. The probability of finding an abnormally excess serum concentration would be almost surreal. We report a peculiar clinical situation that may impact the vitamin B₁₂ immunoassay on the Roche Elecsys 2010 due to excess analyte concentration. In separate episodes (Feb and June 2010), the Biochemistry laboratory of a tertiary-care hospital, Kolkata, India, encountered two critically ill patients with background chronic kidney disease (CKD), low urine output, and on cyanocoabalamin supplementation, who had serum vitamin B₁₂ concentrations far exceeding expected values; even post dialysis. The B₁₂ assays (pmol/l) were performed using electrochemiluminiscence immunoassay on Roche Elecsys 2010, the assay validity confirmed by concomitant quality control runs. The immunoassays failed to deliver results, flagged with “signal level below limit”. Biotin therapy was ruled out as a possible interferent. In the first episode, re-assay of a repeat draw yielded same outcome; outsourcing on Immulite provided concentration of >738 pmol/l. Serial dilution gave result of >29520 pmol/l on Elecsys 2010. In the second, we gained from past experience. Vitamin B₁₂ concentration >59040 pmol/l was conveyed to the treating nephrologist the very day. The B₁₂ immunoassay on the Elecsys 2010 employs sequential incubation steps for competitive binding that is compromised in the event of abnormally excess B₁₂ concentration in patient sera akin to the prozone effect. This knowledge may be beneficial while assaying sera of CKD patients to avoid financial loss due unnecessary repeats and delay in turnaround time.     

运动性横纹肌溶解征并发急性肾衰竭1例报道

运动性横纹肌溶解征并发急性肾衰竭(ARF)是一类较少见的急性肾衰竭。文章通过对剧烈运动致急性肾衰竭1例的发生和临床诊治的报道,旨在加强对本病的认识与预防。